Wyniki wyszukiwania - Biblioteka Nauki

1

Mutations in TLR1 - cause hyporesponsiveness to Mycobacterium avium subsp. paratuberculosis

100%

Bhide M. , Mucha R. , Mikula Jr. I. , Kisova L. , Cepkova M. , Mikula Sr. I.

Prace i Materiały Zootechniczne

|

2009

|

tom 67

2

Susceptibility testing and resistance phenotypes detection in bacterial pathogens using the VITEK 2 system

100%

Stefaniuk E. , Mrowka A. , Hryniewicz W.

|

nr 4

EN

A set of well characterized strains, collected in Polish hospitals, including Gram-negative (n = 93) and Gram-positive (n = 90) isolates was used in the study. The VITEK 2 AST-cards were used in the analysis according to the manufacturer's recommendations. Comparison of the susceptibility data obtained by the standard method and by VITEK 2 cards proved concordant in 99% of cases. Clinically important mechanisms were revealed by the VITEK 2 AES with > 95% agreement with reference data including methicillin resistance in staphylococci (98%), high-level aminoglycoside resistance in enterococci (100%), VanA and VanB phenotypes in enterococci (100%), and ESBLs in Enterobacteriaccae (93.8%). The VITEK 2 AES System appears a reliable tool for the detection and interpretive reading of clinically important mechanisms of resistance and can be recommended for routine work.

3

Structural analyses of Shigella invasion proteins reveals non-conserved; intrinsically unstructured regions

86%

Chakrabarti S. , Ganguli S.

International Letters of Natural Sciences

|

2013

|

tom 05

EN

Shigella is one of the most common bacterial pathogens that are isolated from patients with diarrhea. Various attempts are being made worldwide with encouraging observations; still the emergence of multidrug-resistant Shigella strains and a continuous high disease incidence imply that shigellosis is an unsolved global health problem which can probably be solved only by developing a proper vaccine and a vaccine regime for the disease. The need of the hour is to foster the development of an effective vaccine which should not only serve to improve hygiene but also should be able to curb infections by the pathogen. This goal can only be achieved by gaining proper detailed knowledge underlying Shigella pathogenesis. The analyses of the Shigella invasion proteins which have been long been targeted to be potential candidate vaccines remains an open ended problem and forms the core of this present computational study which identifies the fact that long regions in the structure of the proteins are disordered having no distinct structural conformation; multiple alignments however, did not show any conserved stretches in the disordered regions. The results probably explain the ability of these proteins to interact with multiple cellular proteins and perform a diverse array of functions leading to successful pathogenesis.

4

Survival of Bacterial Pathogen Isolated from Urine in Surface Water in Sebou River Estuary (Morocco)

86%

Ech-Chayeb K. , Auajjar N. , Lihi K. , Attarassi B.

Ecological Engineering & Environmental Technology

|

2023

|

tom Vol. 24, iss. 3

71--78

EN

Hospital effluents are one of the main sources of contamination of groundwater and rivers if they are collected by urban networks and sent to wastewater treatment plants (WWTP), without prior treatment. These effluents are potential vectors of resistant pathogenic bacteria and could contribute to the spread of these strains in the environment. The Sebou River is used as a receptacle for domestic water treatment from the city of Kenitra. The main of the study is to analyze the effect of pH (8.2, 7.5, and 6.5) at a temperature of 22°C on the behavior of three pathogenic bacteria isolated from urine in filtered and sterilized water Sebou River. The water of Sebou River was inoculated with the three bacterial strains tested by a concentration that varied from 106–108 CFU/mL, then incubated for 30 days. The results of the obtained analyses showed that the pH affects the survival of the tested bacterial strains. For some strains, the alkaline pH at 22°C is a beneficial effect that prolongs survival. The main finding from the study was that the three strains of Escherichia coli at a pH of 8.2 have a better survival that reaches the 7th day and then declines. On the other hand, with a strain of Klebsiella pneumoni and Staphylococcus aureus at pH 7.5, the action effects are harmful; it limits survival to 72 hours and causes a rapid decrease in the number of bacteria. We have shown in our experiments that bacteria survive much better at low temperatures and basic pH.

5

Morphostructural damage of foodborne bacterial pathogens influenced by swamp cranberry (Vaccinium oxycoccos L.) pomace extract treatment as revealed by transmission electron microscopy (TEM)

86%

Stobnicka-Kupiec A. , Gniewosz M.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2019

|

tom 598

6

Seroprevalence of selected viral and bacterial pathogens in free-ranging European bison from the Bialowieza Primeval Forest, [Poland]

86%

Salwa A. , Anusz K. , Arent Z. , Paprocka G. , Kita J.

Polish Journal of Veterinary Sciences

|

2007

|

tom 10

|

nr 1

EN

Blood was collected from bison that were selected between 1991 and 2001 for poor body condition, cachexia, lameness and balanoposthitis. Sera were tested for antibodies to bovine herpes virus type 1 (BHV-1), bovine viral diarrhoea virus (BVDV), foot-and-mouth disease virus (FMDV), parainfluenza virus type 3 (PI-3), Brucella abortus, Chlamydophila abortus, Coxiella burnetii, and Leptospira interrogans. The results of serological tests showed a prevalence of low titers of a serological reaction to Chlamydophila abortus (45.1%), bovine viral diarrhoea virus (29.5%), Leptospira interrogans (21.3%) and parainfluenza virus type 3 (13.9%). There were differences in the prevalence of antibodies to Ch. abortus between female and male bison. Futhermore, a relationship between age and antibodies to BVDV was observed in older bison. These results suggest that there is some transmission of bacterial and viral pathogens occurring between domestic and wild ruminants grazing in the same pastures in the peripherial areas of Białowieża Primeval Forest.

7

Coxiellaceae in ticks from human, domestic and wild hosts from Sardinia, Italy: high diversity of Coxiella-like endosymbionts

72%

Chisu V. , Mura L. , Foxi C. , Masala G.

Acta Parasitologica

|

2021

|

tom 66

|

nr 2

8

Iron shortage and bile salts play a major role in the expression of ompK gene in Vibrio anguillarum

72%

Hamod M. A. , Bhowmick P. P. , Shukur Y. N. , Karunasagar I. , Karunasagar I.

Polish Journal of Microbiology

|

2014

|

tom 63

|

nr 1

EN

The outer membrane protein K, OmpK first identified in Vibrio parahaemolyticus has been shown to be a receptor for a broad host range vibriophage KVP40 infecting members of the Vibrionaceae. In the study, the effect of culture conditions on the expression of ompK in V. anguillarum was studied using real-time PCR. The expression increased significantly in the presence of bile salts and iron chelating agent 2, 2’ bipyridine, suggesting a role for this protein in bile resistance and also in iron acquisition by V. anguillarum. OmpK induction by iron limitation and the presence of bile salts was reconfirmed by western blot technique after growing the cells in trypticase soy broth supplemented with bile salts, blood and 2, 2’ bipyridine. We surmise that the expression of OmpK protein of V. anguillarum is bile salt and iron chelating agent-dependent.

9

Monocyte response receptors in BCG driven delayed type hypersensitivity to tuberculin

72%

Strapagiel D. , Kasztalska K. , Druszczynska M. , Kowalewicz-Kulbat M. , Vrba A. , Matusiak A. , Chmiela M. , Rudnicka W.

|

tom 46

|

nr 3

353-359

10

Microbiological safety of food of animal origin from organic farms

72%

Sosnowski M. , Osek J.

Journal of Veterinary Research

|

2021

|

tom 65

|

nr 1

11

Autolysis of Listeria monocytogenes strains isolated from food and clinical specimens

72%

Cibik R. , Cetinkaya F. , Gunes N. , Ozakin C. , Soyutemiz G. E.

Medycyna Weterynaryjna

|

2006

|

tom 62

|

nr 11

1242-1244

EN

Autolysis of Listeria monocytogenes strains isolated from clinical and food specimens were tested under starvation conditions. Late exponential phase harvested bacterial cells were transferred in potassium phosphate buffer (100 mM, pH 7) and incubated at 37°C to induce lysis. At the end of the 48 h incubation period a heterogeneous autolysis profile was observed among the tested strains. All the clinical strains exhibited high autolysis i.e. above 75%. Meanwhile, the extent of food isolates was not as high as the clinical ones but demonstrated a great variability - ranging between 22 to 88%. An increase in the amount of intracellular markers upon lysis was also measured in the higher autolytic clinical strain - UVF172, compared to low autolytic food strain - UVF114. The results obtained from the study provided evidence that autolysis in L. monocytogenes is strain dependent and revealed that tested clinical isolates had a higher level of autolysis than food isolates.

12

Activities of nitrogen metabolism-related enzymes in cucumber leaves infected with Pseudomonas syringae pv.lachrymans

72%

Gajewska E. , Kuzniak E. , Naliwajski M. , Sapinska S. , Wielanek M. , Sklodowska M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

|

tom 94

|

nr 3

13

Genetic background of host-pathogen interaction between Cucumis sativus L. and Pseudomonas syringae pv. lachrymans

72%

Olczak-Woltman H. , Schollenberger M. , Niemirowicz-Szczytt K.

Journal of Applied Genetics

|

2009

|

tom 50

|

nr 1

1-7

EN

The interplay of plant resistance mechanisms and bacterial pathogenicity is very complex. This applies also to the interaction that takes place between the pathogen Pseudomonas syringae pv. lachrymans (Smith et Bryan) and the cucumber (Cucumis sativus L.) as its host plant. Research on P. syringae pv. lachrymans has led to the discovery of specific factors produced during pathogenesis, i.e. toxins or enzymes. Similarly, studies on cucumber have identified the specific types of plant resistance expressed, namely Systemic Acquired Resistance (SAR) or Induced Systemic Resistance (ISR). This paper presents a summary of the current state of knowledge about this particular host-pathogen interaction, with reference to general information about interactions of P. syringae pathovars with host plants.

14

Synergy between novel antimicrobials and conventional antibiotics or bacteriocins

72%

Wolska K. I. , Grzes K. , Kurek A.

Polish Journal of Microbiology

|

2012

|

tom 61

|

nr 2

EN

Due to the alarming spread of resistance to classic antimicrobial agents, innovative therapeutic methods to combat antibiotic-resistant bacterial pathogens are urgently required. This minireview examines the enhancement of antibiotic efficacy by their combination with new antimicrobials, such as plant-derived compounds, metal ions and nanoparticles and bacteriophage lytic enzymes. The mechanisms of the observed synergy are also described. The promising results of basic research indicate that in future, combined therapy may be applied in human and veterinary medicine, agriculture and the food industry to combat bacterial pathogens.

15

Pathogenicity islands and the evolution of microorganisms

72%

Mokracka J.

Seria Biologiczna. Uniwersytet im. Adama Mickiewicza w Poznaniu

|

2005

|

tom 72

16

Molecular aspects of Listeria monocytogenes infection

72%

Krawczyk-Balska A. , Bielecki J.

Polish Journal of Microbiology

|

2004

|

tom 53

|

nr Suppl.

EN

Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection. This review is focused on the role of secreted proteins including listeriolysin O and two distinct phospholipases C, in modulating the signal transduction of infected cells.

17

Simultaneous occurrence of selected food-borne bacterial pathogens on bovine hides, carcasses and beef meat

58%

Wieczorek K. , Osek J.

Polish Journal of Veterinary Sciences

|

2010

|

tom 13

|

nr 4

EN

The aim of this study was to determine the simultaneous occurence of Salmonella spp., L. monocytogenes, verotoxigenic E. coli (VTEC), and Campylobacter spp. in slaughtered cattle and in beef meat subjected for human consumption. A total of 406 bovine hides and 406 corresponding carcasses were used to collect the samples with a swab method after exsanguination and evisceration of animals, respectively. Furthermore, 362 beef meat samples were purchased in local retail shops over the same period of time as for the bovine samples. Food-borne bacterial pathogens were identified with standard ISO methods with some modification by the use of PCR for VTEC. The isolated bacteria were then molecularly speciated (Campylobacter), serotyped (L. monocytogenes) and characterized for the presence of several virulence marker genes (VTEC and Campylobacter). It was found that 49 hide (12.1%) and 3 (0.7%) carcass samples were contaminated with more than one bacterial pathogen tested. Most of the hides were positive for Campylobacter spp. and VTEC (27 samples) and Campylobacter spp. together with L. monocytogenes (12 samples). Eight bovine hides contained L. monocytogenes and VTEC while L. monocytogenes and Salmonella spp. were detected in one sample. Furthermore, 3 pathogens (Campylobacter spp., L. monocytogenes and VTEC) were simultaneously identified in one bovine hide tested. In case of bovine carcasses 2 samples contained Campylobacter spp. and VTEC whereas one carcass was positive for L. monocytogenes and VTEC. On the other hand, 10 out of 362 (2.8%) minced beef samples were contaminated with at least two pathogens tested. The majority of these samples were contaminated with L. monocytogenes and Salmonella spp. (6 samples). It was noticed that equal number of C. jejuni and C. coli were found, irrespective of the origin of the samples. Most of the strains possessed more than one pathogenic factor as identified by PCR. Molecular serotyping of L. monocytogenes revealed that the majority of the isolates (27 out of 31; 87.1%) belonged to 1/2a serogroup. It was found that most of the VTEC isolates possessed the Shiga toxin stx2 gene (12 strains) whereas only 2 strains were stx1-positive. The eneterohemolysin and intimin markers were identified only in 7 and 2 isolates, respectively. PCR analysis revealed that 4 VTEC belonged to O91 serogroup, 2 strains were O145 and 1 isolate was identified as O113. None of the VTEC detected in the study was O157 serogroup.

18

Regulation of enterotoxins gene expression in Staphylococcus aureus strains isolated from milk of cows with mastitis

58%

Nawrotek P. , Fijalkowski K. , Karakulska J. , Czernomysy-Furowicz D.

Advances in Agricultural Sciences

|

2010

|

tom 13

|

nr 1

19

Isolation and identification of vibrio sp. in the hepatopancreas of cultured white pacific shrimp (Litopenaeus vannamei)

58%

Shanmugasundaram S. , Mayavu P. , Manikandarajan T. , Suriva M. , Enswar A. , Anbarasu R.

International Letters of Natural Sciences

|

2015

|

tom 46

EN

The Vibrio is the most common genera with crustaceans often causing various diseases in Aquaculture and significant economic losses. Many Vibrio species are pathogenic to human and have been implicated in food borne diseases. The present study was carried out, the isolation and identification of pathogenic bacterial flora were isolated from infected in hepatopancreas of vannamei. The SPDS Oceanic farm, RS Aqua farm of and Valli vilas Aqua farm Vellar estuary, Cuddalore District, Tamil Nadu, during the period of (September 2013 to November 2013). The collected samples were plated on TCBS- (Thiosulfate-Citrate-Bile salt-Sucrose) agar medium. The present study, totally 253 green colonies were isolated from TCBS agar plates and among these, 175colonies were identified by using the biochemical tests showed the V. parahaemolyticus, V. mimicus, V. vulnificus, V. damsela and P. shigelloides. The maximum species was recorded in V. parahaemolyticus (83.4 %) and minimum was observed in V. mimicus (1.7 %).

20

The potential application of Origanum dubium Boiss. essential oil as a seed protectant against bean and tomato seed-borne bacterial pathogens

58%

Basim H. , Turgut K. , Kaplan B. , Basim E. , Turgut A.

Acta Scientiarum Polonorum. Hortorum Cultus

|

2019

|

tom 18

|

nr 3

EN

Origanum dubium is a valuable wild oregano species of the natural flora of Antalya, Turkey. In this study, we extracted essential oil (EO) by hydro-distillation of the aerial parts of selected O. dubium chemotype with highest EO content, and analyzed by gas chromatography/mass spectrometry. Carvacrol was the primary component (85.9%) among 24 different compounds in the EO. The volatile test showed minimum inhibitory effect of the EO against Xanthomonas axonopodis pv. vesicatoria, Clavibacter michiganensis subsp. michiganensis, Xanthomonas axonopodis pv. phaseoli, Xanthomonas axonopodis pv. phaseoli var. fuscans, and Pseudomonas syringae pv. tomato, at 309, 303, 318, 254, and 901 µL/mL, respectively. A 1-hour treatment of bean and tomato seeds in the volatile phase of the EO effectively sterilized the seeds from bacterial pathogens without inhibiting their germination. To the best of our knowledge, this is the first study on the potential for the use of O. dubium EO as a seed protectant against bean and tomato seed-borne bacterial pathogens.