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1 Bulletin of the Veterinary Institute in Puławy

1 2010

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Incidence and detection of aviadenoviruses of serotypes 1 and 5 in poultry by PCR and duplex PCR

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Niczyporuk J. , Samorek-Salamonowicz E. , Czekaj H.

Bulletin of the Veterinary Institute in Puławy

2010

tom 54

nr 4

The aim of the study was to develop and optimise a single PCR and duplex PCR for the detection and differentiation of avian adenovirus serotypes 1 and 5 in poultry. The methods were based on sequence of hexon gene of the serotypes. The PCR and duplex PCR DNA products were 227 pb and 178 bp for serotypes 1 and 5, respectively, and were highly specific. The duplex-PCR method was found to be a highly specific, sensitive, and efficient rapid tool for the detection of serotypes 1 and 5 of adenoviruses, which could be detected in one reaction mixture. During this study, 30 of the isolates obtained from internal organs of healthy birds contained both serotypes of the adenovirus. In the case of six isolates, both serotypes were detected in one bird.