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Wielocukry O-swoiste Proteus mirabilis.

100%

Kaca W. , Literacka E. , Cieslikowski T. , Knirel Y. A.

tom 09

42-43

Pierwszorzędowa struktura antygenów O bakterii rodzaju Salmonella

72%

Gajdus J. , Głośnicka R. , Szafranek J.

Wiadomości Chemiczne

2006

tom [Z] 60, 9-10

621-653

Salmonella spp. are pathogenic Gram-negative bacteria that belong to Enterobacteriaceae family with lipopolysaccharide (LPS) as a constituent of cell wall. This is an integral component of the outer membrane of the wall. Salmonella smooth (S) forms produce LPS, which is composed of three parts, chemically bonded together viz. polysaccharide O-antigen, oligosaccharide core region and lipid A. Antigens O (O-PS) together with H flagella antigens are the foundation of serological classification of these bacteria. O-chain, which is built with up to 50 oligosaccharide repeating units, is one of the products of mild acidic hydrolysis of LPS. Due to the fact that polysaccharide antigens are the sites of specific antibody complexing, any difference in primary and secondary structures of O-antigens reflect serological specificity of bacteria. Taking this fact into consideration, we can distinguish about 2541 Salmonella serotypes with O and H antigenic formulas defined [4]. In this review we present 55 chemical structures of O-antigenic repeating units of Salmonella strains including their heterogeneity structures. The structures can have 22 different monosaccharide residues usually in 3 to 6 sugar repeating units. We describe here selected chemical and spectroscopic (MS, NMR) methods for primary structure examination of these bacterial O-PS. Enzymatic and immunochemical methods are also described. Cross-reactions of Salmonella spp. with any other bacteria or blood group A, B, 0 antigens are explained on the molecular level. Thus, structural assignments of somatic antigens of Salmonella spp. allow us to understand the molecular level of the classification system of these bacteria.

Wystepowanie wybranych genow loci waa i wb zwiazanych z wytwarzaniem lipopolisacharydu u referencyjnych i izolowanych z materialu klinicznego szczepow paleczek Klebsiella pneumoniae

58%

Gierczynski R. , Kaluzewski S. , Zasada A. A. , Rastawicki W. , Jagielski M.

nr 4

383-393

Określono częstość występowania 11 wybranych genów loci waa i wb związanych z biosyntezą oligocukru rdzenia lipopolisacharydu (LPS) i antygenu O pałeczek Klebsiella. Zbadano 26 wybranych szczepów wzorcowych dla antygenu K reprezentujących wyróżniane obecnie grupy serologiczne pałeczek K. pneumoniae a także 19 szczepów epidemicznych i izolowanych od przypadkowych osób na terenie kraju. Zróżnicowanie częstości występowania poszukiwanych genów w grupie badanych szczepów stanowiło podstawę wyodrębnienia 21 genotypów. Wykazano przydatność genów z loci waa i wb do genotypowania pałeczek K. pneumoniae.

The goal of presented study was to determine by PCR differences in existence or homology level of selected genes involved in A', pneumoniae lipopolysaccharide (LPS) synthesis and application of obtained results for genotyping. Number of 26 reference strains of K. pneumoniae belonging to sero- groups 01,02a, 02a2e, 02a2e2h, 02a2f2g, 03,04,05,07,08, and 012 was tested together with 13 epidemic strains from 5 outbreaks and б casual isolates for the existence of 7 (waaA, waaE, waaL, waaQ, waaZ, waaX and uge) and 4 (wbdA, wbdC, manB, wbbO) genes of the waa and wb clusters for LPS biosynthesis. Based on PCR results, 10 and 11 genotypes were distinguished in tested strains for genes from waa and wb clusters respectively. Derived dendrograms were topologically dissimilar, however observed correlation between clonal groups and O-group was marginal for both compared clusters. Since we aimed to develop genotyping method for K. pneumoniae, genes from clusters waa and wb were used together to enhance the distinguishing capacity. Twenty-one genotypes were distinguished in 45 tested strains (DI=0,46) when 11 genes were applied for typing. Although no apparent correlation between genotype and serogroup was observed, epidemic isolates from 5 outbreaks were diversified into 5 genotypes, whereas strains from the same outbreak were indistinguishable. Described here genotyping method is determinative and was found time and cost effective. This method may be applied in every clinical laboratory equipped in an ordinary PCR apparatus.

Influence of O-antigen Aeromonas salmonicida on non-specific and specific immune responses in Siberian sturgeon, Acipenser baeri Brandt

58%

Kolman H. , Siwicki A. K. , Kolman R.

nr 1

93-102

Czynniki chorobotworczosci paleczek z rodzaju Proteus

51%

Rozalski A.

tom 31

nr 3-4

205-224