A liquid chromatography-fluorescence detection method for the determination of five fluoroquinolones in muscle tissue was proposed. The method is selective and robust enough for the required purposes. The whole procedure was validated in accordance with the Commission Decision 657/2002/EC. Detection capabilities were from 132.8 to 421.0 µg/kg, and recoveries ranged from 51.7% to 80.91%, depending on the analyte.