Wyniki wyszukiwania - Biblioteka Nauki

1

Proangiogenic activity of plant extracts in accelerating wound healing - a new face of old phytomedicines

100%

Majewska I. , Gendaszewska-Darmach E.

Acta Biochimica Polonica

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2011

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tom 58

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nr 4

449-460

EN

Angiogenesis, the formation of new capillaries from pre-existing vascular network, plays an important role in physiological and pathological processes such as embryonic development, wound healing, and development of atherosclerosis. Extension of the circulatory network is also considered to be one the most important factors during cancerogenesis. Inhibition of angiogenesis may lead to inhibition of tumor growth whereas stimulation may improve wound healing. Research achievements suggest the use of plants and their extracts as potential therapeutic agents with pro- or antiangiogenic activity. Since the anticancer and antiangiogenic properties of many phytomedicines have been amply reviewed elsewhere this paper will focus on the treatment of vascular insufficiency in wound healing. Globally accepted herbal drugs are thought to be safe and effective, however, there is a need for more evidence-based confirmation in controlled and validated trials. Among the most frequently studied proangiogenic phytochemicals are ginsenosides from Panax ginseng, beta-sitosterol from Aloe vera, calycosin from Radix Astragali, and extracts from Hippophae rhamnoides L. and Angelica sinensis.

2

Ovarian cancer: early detection, angiogenesis, lymphangiogenesis and current prospects for therapy

100%

Wertel I. , Bednarek W. , Czekierdowski A. , Kotarski J.

Polish Journal of Public Health

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2015

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tom 125

|

nr 1

24-28

EN

I Chair and Department of Gynecological Oncology and Gynecology is a specialist research center providing help in diagnostics and treatment of gynecological malignancies. The research work is focused on the processes of angiogenesis and lymphangiogenesis. Development of blood and lymphatic vessels is subject to research in a wide range of malignancies, including ovarian cancer, endometrial cancer and uterine sarcomas. Angiogenesis in malignancies of the female genital tract is investigated by using some modern 3D sonography that uses high-definition blood flow imaging. Ovarian Tumors and Early Ovarian Cancer Detection unit was established in 2002 and since that time more than 3500 patients with difficult to diagnose tumors have been consulted and treated in the Department. Ovarian cancer immunology studies are the second leading research fiekld in the 1st Chair Department of Gynecological Oncology and Gynecology. The Department is well equipped with diagnostic devices as well as a scientific laboratory. This allows for studies in the fields of imaging of masses, their immunology, biochemistry and molecular biology. Understanding immunological response in patients with ovarian cancer is the key to develop new, effective therapies, including immunological vaccines. In this area we are cooperating with prominent international research centers: Department of Surgery, University of Michigan and Department of Microbiology and Immunology, University of Arkansas. Results of our research are published in both Polish and international journals specializing in fields of gynecology, oncology, immunology and basic science.

3

Combined delivery of an antiangiogenic protein (angiostatin) and an immunomodulatory gene (interleukin-12) in the treatment of murine cancer.

100%

Wilczyńska U. , Kucharska A. , Szary J. , Szala S.

Acta Biochimica Polonica

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2001

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tom 48

|

nr 4

1077-1084

EN

We investigated the feasibility of a novel therapeutic approach to treat neoplastic diseases in mice. This novel strategy consists in delivering a protein (angiostatin) with strong antiangiogenic properties, followed by administration of the interleukin 12 gene that is strongly immunomodulatory and has also some antiangiogenic effects. When angiostatin-mediated antiangiogenic therapy was used in combination with intratumor delivery of the IL-12 gene (a strategy much safer than IL-12 protein administration), this produced a synergistic therapeutic effect.

4

Role of vascular endothelial growth factor in ovarian physiology - an overview

100%

Kaczmarek M. M. , Schams D. , Ziecik A. J.

Reproductive Biology

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2005

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tom 05

|

nr 2

5

Pro-angiogenic effects of X-rays on murine endothelial cells

100%

Lisiak E. , Dziekiewicz M. , Meineke V. , Bilski M. , Janiak M.

Nukleonika

|

2005

|

tom Vol. 50,suppl.2

17-20

EN

Recently, significant attention has been paid to the possibility of thwarting cancer progression by inhibition of neoangiogenesis (formation of new blood vessels) in growing tumors. Although general mechanisms of angiogenesis have been elucidated, virtually nothing is known about the effects of low doses of ionizing radiation on pro-angiogenic properties of endothelial cells. In the present study, we evaluated the effects of a low (0.2 Gy), intermediate (1 Gy), and high (4 Gy) doses of X-rays on a few angiogenesis-related parameters of isolated murine endothelial cells. We show here that 24 to 48 hours after irradiation with 0.2 Gy the cell proliferation was inhibited to a similar extent as after the exposure to 1 Gy. Also, adhesion of the 0.2 Gy-irradiated cells to both gelatin and MatrigelŽ was inhibited 24 hours post-exposure, whereas irradiation with 1 or 4 Gy resulted in the increased adhesion of the cells to these substrata. Similar effects were observed during the "wound" migration assay. Finally, 24 hours after exposure of the cells to 0.2 Gy of X-rays, the surface expression of the â3 integrin subunit was down-regulated, whereas irradiations with 1 and 4 Gy of X-rays resulted in the significantly elevated expression of this subunit. These results indicate that proliferating endothelial cells are sensitive in vitro to relatively low doses of ionizing radiation

6

Construction of a bicistronic proangiogenic expression vector and its application in experimental angiogenesis in vivo.

100%

Maciej Małecki; , Przybyszewska M. , Janik P.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr 3

875-882

EN

Manipulation of angiogenesis in vivo is an example of successful gene therapy strategies. Overexpression of angiogenic genes like VEGF, FGF or PDGF causes new vessel formation and improves the clinical state of patients. Gene therapy is a very promising procedure but requires large amounts of pharmaceutical-grade plasmid DNA. In this regard we have constructed a bicistronic plasmid DNA vector encoding two proangiogenic factors, VEGF165 and FGF-2. The construct (pVIF) contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV) which permits both genes to be translated from a single bicistronic mRNA. The IRES sequence allows for a high efficiency of gene expression in vivo. The pVIF vector was characterized in vitro and in vivo. In vivo angiogenesis studies showed that the bicistronic vector encoding two proangiogenic factors induces the formation of new vessels significantly more than pVEGF165 or pFGF-2 alone. In our opinion the combined proangiogenic approach with VEGF165 and FGF-2 is more powerful and efficient than single gene therapy. We also postulate that IRES sequence can serve as a useful device improving efficiency of gene therapy.

7

ALA-PDT of skin malignancies affects surrogate biomarkers of angiogenesis: a clinical study

100%

Adamek M. , Sieroń A. , Kawczyk-Krupka A. , Wiczkowski A. , Król W. , Szygula M. , Cebula W. , Zieleznik W. , Biniszkiewicz T. , Niepsuj K. , Czuba Z. , Kasperczyk S.

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

|

2004

|

tom Vol. 10, nr 1-2

5-5

8

GPU enhanced simulation of angiogenesis

100%

Worecki M. , Wcisło R.

Computer Science

|

2012

|

tom Vol. 13 (1)

35-48

EN

In the paper we present the use of graphic processor units to accelerate the most time-consuming stages of a simulation of angiogenesis and tumor growth. By the use of advanced CUDA mechanisms such as shared memory, textures and atomic operations, we managed to speed up the CUDA kernels by a factor of 57x. However, in our simulation we used the GPU as a co-processor and data from CPU was copied back and forth in each phase. It decreased the speedup of rewritten stages by 40%. We showed that the performance of the entire simulation can be improved by a factor of 10 up to 20.

9

Combination of vasostatin gene therapy with cyclophosphamide inhibits growth of B16(F10) melanoma tumours

88%

Jazowiecka-Rakus J. , Jarosz M. , Szala S.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr 1

199-202

EN

Angiogenesis, i.e. formation of new blood vessels out of pre-existing capillaries, is essential to the development of tumour vasculature. The discovery of specific antiangiogenic inhibitors has important therapeutic implications for the development of novel cancer treatments. Vasostatin, the N-terminal domain of calreticulin, is a potent endogenous inhibitor of angiogenesis and tumour growth. In our study, using B16(F10) murine melanoma model and electroporation we attempted intramuscular transfer of human vasostatin gene. The gene therapy was combined with antiangiogenic drug dosing schedule of a known chemotherapeutic (cyclophosphamide). The combination of vasostatin gene therapy and cyclophosphamide administration improved therapeutic effects in melanoma tumours. We observed both significant inhibition of tumour growth and extended survival of treated mice. To our knowledge, this is one of the first reports showing antitumour efficacy of electroporation-mediated vasostatin gene therapy combined with antiangiogenic chemotherapy.

10

The effect of hydrazine derivatives of 3-formylchromones on angiogenic basic fibroblast growth factor and fibroblast growth factor receptor-1 in human melanoma cell line WM-115

88%

Łazarenkow A. , Michalska M. , Mirowski M. , Słomiak K. , Nawrot-Modranka J.

Acta Biochimica Polonica

|

2017

|

tom 64

|

nr 3

585-590

EN

The hydrazine derivatives of benzopyrones remain an unexplored group of chemical compounds. This preliminary study investigates the influence of A-5, CH-3 and K-2 derivatives at concentrations of 1, 10, 100 nM and 1 μM on selected biochemical factors of a melanoma cell line WM-115, with regard to their potential angiogenic properties. The studied compounds were found to influence cell proliferation, as well as total protein, bFGF and FGFR1 concentration.

11

Endoglin (cd105) and S100A13 as markers of active angiogenesis in endometriosis

88%

Hayrabedyan S. , Kyurkchiev S. , Kehayov I.

Reproductive Biology

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2005

|

tom 05

|

nr 1

12

Carbon monoxide - a "new" gaseous modulator of gene expression.

88%

Dulak J. , Józkowicz A.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr 1

31-47

EN

Carbon monoxide (CO) is an odorless, tasteless and colorless gas which is generated by heme oxygenase enzymes (HOs). HOs degrade heme releasing equimolar amounts of CO, iron and biliverdin, which is subsequently reduced to bilirubin. CO shares many properties with nitric oxide (NO), an established cellular messenger. Both CO and NO are involved in neural transmission and modulation of blood vessel function, including their relaxation and inhibition of platelet aggregation. CO, like NO, binds to heme proteins, although CO binds only ferrous (FeII) heme, whereas NO binds both ferrous and ferric (FeIII). CO enhances the activity of guanylate cyclase although it is less potent than NO. In contrast, CO inhibits other heme proteins, such as catalase or cytochrome P450. The effects of CO on gene expression can be thus varied, depending on the cellular microenvironment and the metabolic pathway being influenced. In this review the regulation of gene expression by HO/CO in the cardiovascular system is discussed. Recent data, derived also from our studies, indicate that HO/CO are significant modulators of inflammatory reactions, influencing the underlying processes such as cell proliferation and production of cytokines and growth factors.

13

Angiogenesis as a prognostic factor in invasive carcinoma of the uterine cervix

88%

Lenczewski A. , Terlikowski S. , Famulski W. , Sulkowska M. , Kulikowski M.

Folia Histochemica et Cytobiologica

|

2001

|

tom 39

|

nr 2

14

Endothelial progenitor cells as a new agent contributing to vascular repair

88%

Miller-Kasprzak E. , Jagodzinski P. P.

Archivum Immunologiae et Therapiae Experimentalis

|

2007

|

tom 55

|

nr 4

15

Construction of a bicistronic proangiogenic expression vector and its application in experimental angiogenesis in vivo

88%

Malecki M. , Przybyszewska M. , Janik P.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr 3

EN

Manipulation of angiogenesis in vivo is an example of successful gene therapy strategies. Overexpression of angiogenic genes like VEGF, FGF or PDGF causes new vessel formation and improves the clinical state of patients. Gene therapy is a very promising procedure but requires large amounts of pharmaceutical-grade plasmid DNA. In this regard we have constructed a bicistronic plasmid DNA vector encoding two proangiogenic factors, VEGF165 and FGF-2. The construct (pVIF) contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV) which permits both genes to be translated from a single bicistronic mRNA. The IRES sequence allows for a high efficiency of gene expression in vivo. The pVIF vector was characterized in vitro and in vivo. In vivo angiogenesis studies showed that the bicistronic vector encoding two proangiogenic factors induces the formation of new vessels significantly more than pVEGF165 or pFGF-2 alone. In our opinion the combined pro- angiogenic approach with VEGF165 and FGF-2 is more powerful and efficient than single gene therapy. We also postulate that IRES sequence can serve as a useful device improving efficiency of gene therapy.

16

Biological properties of human skeletal myoblasts genetically modified to simultaneously overexpress the pro-angiogenic factors vascular endothelial growth factor-A and fibroblast growth factor-4

75%

Zimna A. , Janeczek A. , Rozwadowska N. , Fraczek M. , Kucharzewska P. , Rucinski M. , Mietkiewski T. , Kurpisz M.

Journal of Physiology and Pharmacology

|

2014

|

tom 65

|

nr 2

17

Ligands of peroxisome proliferator-activated receptor-γ increase the generation of vascular endothelial growth factor in vascular smooth muscle cells and in macrophages.

75%

Jozkowicz A. , Dulak J. , Piatkowska E. , Placha W. , Dembinska-Kiec A.

Acta Biochimica Polonica

|

2000

|

tom 47

|

nr 4

1147-1157

EN

Peroxisome proliferator-activated receptors-γ (PPARγ) are ligand-inducible transcription factors of the nuclear hormone receptor superfamily. We examined the effect of PPARγ activation on the generation of vascular endothelial growth factor (VEGF), one of the major angiogenic agents. Rat vascular smooth muscle cells (VSMC) and murine macrophages RAW264.7 were incubated for 24 h with PPARγ activators: prostaglandin J2 and ciglitazone. PPARγ were expressed in VSMC and RAW cells and their activity was upregulated in the presence of PGJ2 and ciglitazone. Incubation of the cells with PPARγ activators significantly augmented the release of VEGF protein into the media, both in resting and in IL-1β- or LPS-stimulated cultures. The higher protein generation was connected with the increased expression of mRNA and transcriptional activation of VEGF promoter. We conclude that the activation of PPARγ upregulates the generation of VEGF and may be involved in the regulation of angiogenesis.

18

Plasminogen activator inhibitor type-1 controls the process of the in vitro sprout formation

75%

Chorostowska-Wynimko J. , Skrzypczak-Jankun E. , Jankun J.

|

nr 3

49-56

EN

Plasminogen activator inhibitor type-1 (PAI-1), the primary regulator of plasminogen activator - urokinase (uPA) plays a crucial role in the cell adhesion and migration and in angiogenesis. We had previously demonstrated that PAI-1 - endothelial cell interplay is critical for the formation of new blood vessels and the process is mostly conducted via uPA- anti-proteinase interaction. In the present study we wished to further examine the role of PAI-1 in the sprout formation, representing the first step of new capillary vessels development by evaluating the effect of PAI-1 on the sprout area. We addressed the issue by assessing the influence of cysteine-mutated PAI-1 proteins characterized by a prolonged half-life time (hDßT - T1/2= 63.59 h and ßT- T1/2= 6931.47 h), and therefore more stable anti-uPA activity, on the appearance of newly formed sprouts. We found that both CysPAI-1 proteins significantly diminished the mean sprout area in a concentration-dependent fashion. The inhibitory effect present in the two examined endothelial cells systems of different origin and functional characteristics - human umbilical vein endothelial cells (HUVEC) and human lung microvascular endothelial cells (HLMVEC) cultures - was noticeably greater for HLMVEC -high urokinase-producers. Moreover, the inhibition rate was significantly greater for the ßT mutant than that for the hDßT PAI-1 mutant in all examined doses (P<0.002), proving a key role of anti-proteinase activity for this effect. Therefore, we concluded that PAI-1 apart from the total sprout length affects also sprouts area in the in vitro sprout formation angiogenesis assay. This effect was achieved mainly via PAI-1's anti-proteinase activity.

19

Osteophyte development during osteoarthritis (OA) : consideration of angiogenesis, mechanical loading and tissue microstructure

75%

Bednarczyk E. , Lekszycki T.

Engineering Transactions

|

2016

|

tom Vol. 64, iss. 4

533--540

EN

This paper is devoted to modelling and investigation of the effects of mechanical loading, blood vessels development and tissue microstructure in osteoarthritis (OA) – a degenerative joint disease. OA is one of the most common diseases affecting the population, and therefore it is a social and medical problem of utmost importance. It predominantly affects the elderly but also sportsmen, obese people and those with curvature of the spine. Although the phenomenon of OA is not yet fully understood, it is commonly accepted that mechanical aspects are crucial in its evolution [1, 2]. Mechanical overloading leads to chondrocytes apoptosis which increases generation of vascular endothelial growth factors [3] then expansion of angiogenesis and osteophyte onset. A properly formulated mathematical model of cartilage degeneration and osteophytes development can significantly help in understanding the complexity of this process. The presented model reflects the most important aspects of the interactions between mechanical and biological factors, crucial for the phenomenon of OA.

20

Anatomical variations in the branches of the human aortic arch: a recent study of a South Australian population

75%

Bhatia K. , Ghabriel M. N. , Henneberg M.

Folia Morphologica

|

2005

|

tom 64

|

nr 3

XX

Variations of the branches of the aortic arch are likely to occur as a result of the altered development of certain branchial arch arteries during the embryonic period of gestation. In the present investigation the pattern of branches of the aortic arch was studied in 81 cadavers from a recent South Australian population of European descent, who have migrated to (n = 38) or were born and lived in (n = 43) South Australia during the twentieth century. Two principal variations were noted in the present study. Firstly, in 6 cadavers, the left vertebral artery originated directly from the arch of the aorta, between the left common carotid and the left subclavian arteries. The 6 subjects were among the subgroup born in South Australia, giving an incidence of 13.95%, which is much higher than in previous reports. The overall incidence of 7.41%, when related to the whole group, is also higher than incidences reported in other populations. The presence of this variation suggests that in some individuals part of the aortic arch is formed from the left 7th inter-segmental artery. Secondly, none of the cadavers examined had the thyroidea ima artery, contrasting with previously reported incidences that varied between 4% and 10%. Since all 6 cadavers with the left vertebral artery variant were born in South Australia, it is suggested that environmental factors may have contributed to this variation. Significant environmental changes in South Australia around the turn of the twentieth century are discussed. This study represents the first systematic investigation of the branches of the aortic arch in a South Australian population and provides data relevant to the practice of medicine.