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EN
Cyanobacteria are natural components of many freshwater bodies worldwide. In Russian lakes, the presence of potentially toxic cyanobacteria was also frequently observed. Our study was conducted in Sestroretskij Razliv Lake (Razliv) and Lower Suzdal Lake (Suzdal) in Saint Petersburg region, Northwestern Russia, which differ from one another in eutrophic status and composition of the phytoplankton community. In large, shallow, artificial and hypertrophic Razliv, Aphanizomenon flos-aquae and Microcystis spp. dominated. Fourteen microcystin variants were identified in this lake. The maximum concentration of extracellular microcystins was 41.37 μg l−1. In eutrophic and shallow Suzdal, dominated by Planktothrix agardhii, nine microcystin variants and anatoxin-a (<0.54 μg l−1) were found. The maximum total concentration of extracellular MCs in this lake was 2.89 μg l−1. Regular studies into the production of cyanotoxins in these water bodies were carried out for the first time. The analyses performed with the application of high-resolution tandem mass spectrometry revealed the presence of microcystins in 59% of the samples collected during a 3-year study. Since both lakes are used for recreational purposes, the regular monitoring program should be implemented to protect water users from a potential risk that was identified in our study.
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EN
Hepatotoxic cyclic peptides and neurotoxic alkaloids are the most common groups of cyanobacterial toxins. They pose a serious threat to human and animal health; each year they are responsible for several cases of animal fatalities (mammals, fish and birds). In Polish water bodies toxic blooms of cyanobacteria are also frequently observed. In this work, a procedure for anatoxin-a analysis has been modified to obtain a sensitive and selective method for routine studies of bloom samples. The presence of cyanobacterial neuro and hepatotoxins in fresh and brackish waters of Pomorskie Province was examined. Hepatotoxins, microcystins or nodularin, were detected in all phytoplankton samples dominated by the cyanobacteria of Microcystis and Anabaena genera or by Nodularia spumigena. HPLC-PDA analysis showed the presence of anatoxin-a only in one sample collected in coastal waters of the Gulf of Gdańsk and dominated by Anabaena.
EN
Nine lakes (Garda, Maggiore, Como, Iseo, Lugano, Idro, Pusiano, Ledro, and Levico) located in the Italian subalpine lacustrine district were chosen for a comparative study of the diversity and distribution of cyanobacterial toxins. All nine lakes are known to host different toxic cyanobacteria, mainly Planktothrix rubescens. An analytical protocol has been used, based on LC-MS technique, for determining 20 different toxins (14 microcystins, 2 nodularins, anatoxin-a and 3 cylindrospermopsins). In the investigation, microcystins were found in all lakes on all sampling dates. Anatoxin-a could be detected in 6 lakes, but it was only present in 4 lakes on all sampling dates (lakes Garda, Iseo, Como, and Maggiore). Nodularins and cylindrospermopsins were not detected at all. The number of microcystins detected in the survey was 9, but 4 were the most abundant (dmRR, YR, dmLR, and LR); they were differently distributed among the lakes, thus providing each lake a unique toxic potential. Statistical analysis showed positive correlations between total microcystin concentrations and eutrophication indicators and also between anatoxin-a concentrations and water temperature, suggesting that anthropic and climate-related stressors can have different impacts on the presence of different cyanotoxins.
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