Wyniki wyszukiwania - Biblioteka Nauki

1

The effect of glycemic control on CEA, CA 19-9, amylase and lipase levels

100%

Ata N. , Dal K. , Kucukazman M. , Yeniova A. Ö. , Karakaya S. , Unsal O. , Dagdeviren M. , Akın K. O. , Baser S. , Beyan E. , Ertugrul D. T.

Open Medicine

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2014

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tom 10

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nr 1

EN

Background: Diabetes mellitus is closely related to pancreas cancer. In this study we aimed to investigate the effect of hyperglycemia on tumor and inflammation markers, as well as pancreatic exocrine functions.Methods: A total of 98 consecutive diabetic patients with poor glycemic control, and 50 healthy controls were included in the study. We measured hsCRP, erythrocyte sedimentation rate (ESR), CA19-9, CEA, amylase and lipase in addition to routine biochemistry tests, before and after euglycemia was achieved.Results: Fasting blood glucose, HbA1c, CA19-9,CEA, hsCRP, ESR, triglycerides, AST, ALT, GGT, ALP, total cholesterol and LDL-C levels decreased significantly with the regulation of glycemic control. Amylase and lipase levels increased with the regulation of glycemic control. After glycemic control, CA19-9 and CEA levels were still higher, whereas amylase and lipase levels were still lower in the diabetic group compared with the control group. Basal HbA1c showed significant correlation with CA19-9, CEA, amylaseand lipase.Conclusions: We propose to repeat observations of tumor markers after hyperglycemia is resolved, in order to avoid unnecessary invasive tests. Our data also suggest that pancreatic exocrine function was improved with lowering blood glucose in a short period of time.

2

Oxidation of activated carbon fibre and its adsorption of amylase

100%

Li Y. , Hu S.

Materials Science Poland

|

2009

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tom Vol. 27, No. 2

453--461

EN

Activated carbon fibre was oxidized by combustion of nitrocellulose and oxidation with nitric acid to introduce the nitrogen and oxygen groups on its surface. The pore structure and chemical groups were investigated by nitrogen adsorption and FTIR. The result showed that the pore structure and surface morphology were not much affected but amide and hydroxyl groups could be introduced after oxidation. The adsorption capacity of amylase was markedly increased, and the hydrolysis activity of starch was maintained after 3 runs.

3

The relationship of biochemical parameters and radiological parameters in the evaluation of the clinical severity of acute pancreatitis in the emergency department – a retrospective analysis

100%

Tortum F. , Tekin E. , Aydın F. , Özdal E. , Tatlısu K.

European Journal of Clinical and Experimental Medicine

|

2023

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nr 2

277-282

EN

Introduction and aim. Computed tomography severity index (CTSI) and Balthazar score are among the most frequently used scorings in the determination of severe acute pancreatitis. The primary purpose of this study is evaluation of the effects of biochemical parameters, Balthazar score and CTSI on mortality in acute pancreatitis. At the same time, correlations with biochemical parameters, CTSI and Balthazar score were evaluated in patients with AP. Material and methods. In this study, the amylase, lipase, CRP, and procalcitonin values of patients diagnosed with acute pancreatitis were retrospectively recorded. Contrast-enhanced computed tomography (CECT) images obtained at the time of presentation to the emergency department or within seven days of admission were re-evaluated by two radiologists. The CTSI scores and Balthazar scores of the patients were calculated. Results. The study included 240 patients. The amylase level of the patients was positively correlated with the Balthazar score at a statistically significant level (R=0.189, p=0.003). In addition, the relationship between pancreatic scoring systems and mortality, the AUC value for CTSI was 0.9 (95% CI: 0.826-0.973) and was higher than other scoring systems. Conclusion. CTSI had better performance in the prediction of mortality in patients with acute pancreatitis.

4

Changes of amylase levels in the acute experimental pancreatitis treated with fibrolan

100%

Madej B. , Anasiewicz A. , Maciejewski R.

Folia Morphologica

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1996

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tom 55

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nr 4

5

Polycarbonate biodegradation by newly isolated Bacillus strains

86%

Arefian M. , Tahmourespour A. , Zia M.

Archives of Environmental Protection

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2020

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tom Vol. 46, no. 1

14--20

EN

As polycarbonate is frequently used in many products, its accumulation in landfills is absolutely harmful to the environment. The aims of this study were the screening and isolation of polycarbonate-degrading bacteria (PDB) and the assessment of their ability in the degradation of polycarbonate (PC) polymers. Nine-month buried- -PC films were used for PDB isolation and identification. The biodegradation ability of the isolates was determined by growth curve, clear zone formation, lipase and amylase production, AFM and FTIR. Bacillus cereus and Bacillus megaterium were identified and considered as PDB. The degradation ability of B. megaterium was significantly higher than that of B. cereus. Both were lipase and amylase positive. AFM and FTIR results showed the initiation of bacterial attachment. The PC biodegradation ability of isolates can be very efficient. Finding such efficient isolates (which was less studied before) will promise a decrease in plastic contamination in the future.

6

Characterization of the amylolytic properties of the rumen ciliate protozoan Eudiplodinium maggii

86%

Belzecki G. , Newbold C. J. , McEwan N. R. , McIntosh F. M. , Michalowski T.

Journal of Animal and Feed Sciences

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2007

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tom 16

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nr 4

590-606

7

Statistical optimization of alpha-amylase production by Streptomyces erumpens MTCC 7317 cells in calcium alginate beads using response surface methodology

86%

Kar S. , Ray R. C.

Polish Journal of Microbiology

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2008

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tom 57

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nr 1

EN

α-Amylase has a wide range of applications in starch industries, i.e. baking, brewing, distillery, etc. The α-amylase production from Streptomyces erumpens MTCC 7317 immobilized cells was compared with that of free cells. The immobilized cells of S. erumpens in calcium alginate beads were more effective for production of α-amylase (12.2% more yield) than free cells. Response surface methodology (RSM) was used to evaluate the effect of main variables, i.e. incubation period, pH and temperature on enzyme production with immobilized cells. A full factorial Central Composite Design (CCD) was applied to study these main factors that affected α-amylase production. The experimental results showed that the optimum incubation period, pH and temperature were 36 h, 6.0 and 50°C, respectively for immobilized cells. Repeated batch fermentation of immobilized cells in shake flasks carried out in starch-beef extract medium showed that S. erumpens cells were physiologically active on the support even after four cycles of fermentation.

8

Degradation of starch granules by amylases

86%

Slominska L. , Klisowska M. , Grzeskowiak A.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

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2003

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tom 06

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nr 2

EN

Production of enzymatic starch hydrolyzates is made by two-steps action of amylolytic enzymes on gelatinized starch granules at the beginning by liquefying enzymes and next by saccharifying enzymes. Differences of optimal parameters of the enzyme action require the change of reaction conditions: temperature and pH.

9

Involvement of ROS and its scavenging enzymes in the response of dormant Avena fatua L. caryopses to karrikinolide (KAR1)

72%

Cembrowska-Lech D. , Kepczynski J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

|

nr 2

10

Gullo’s syndrome – case report

72%

Rycyk A. , Furtak P. , Madro A. , Kasztelan-Szczerbinska B. , Cichoz-Lach H.

Journal of Pre-Clinical and Clinical Research

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2020

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tom 14

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nr 4

11

Effect of metabolic disorders accompanying gastroenteritis on the pancreatic exocrine function in piglets. B. Disturbances of the pancreatic exocrine function in piglets with gastroenteritis

72%

Rychlik A.

Polish Journal of Veterinary Sciences

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2002

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tom 05

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nr 3

12

AMY2B gene copy-number variation studied by droplet digital PCR (ddPCR) in three canids: red fox, arctic fox, and Chinese raccoon dog

72%

Antkowiak M. , Nowacka-Woszuk J. , Szczerbal I. , Switonski M. , Szydlowski M.

Folia Biologica

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2020

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tom 68

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nr 2

13

Screening and characterization of thermo-active enzymes of biotechnological interest produced by thermophilic Bacillus isolated from hot springs in Tunisia

72%

Thebti W. , Riahi Y. , Gharsalli R. , Belhadj O.

Acta Biochimica Polonica

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2016

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tom 63

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nr 3

581-587

EN

As part of the contribution to the global efforts in research of thermostable enzymes being of industrial interest, we focus on the isolation of thermophilic bacteria from Tunisian hot springs. Among the collection of 161 strains of thermophilic Bacillus isolated from different samples of thermal water in Tunisia, 20% are capable of growing at 100°C and the rest grow at 70°C or above. Preliminary activity tests on media supplemented with enzyme-substrates confirmed that 35 strains produced amylases, 37 - proteases, 43 - cellulases, 31 - xylanases and 37 - mannanases. The study of the effect of temperature on enzyme activity led to determination of the optimal temperatures of activities that vary between 60 and 100°C. Several enzymes were active at high temperatures (80, 90 and 100°C) and kept their activity even at 110°C. Several isolated strains producing enzymes with high optimal temperatures of activity were described for the first time in this study. Both strains B62 and B120 are producers of amylase, protease, cellulase, xylanase, and mannanase. The sequencing of 16S DNA identified isolated strains as Geobacillus kaustophillus, Aeribacillus pallidus, Geobacillus galactosidasus and Geobacillus toebii.

14

Ostre zapalenie trzustki u 15‑letniej dziewczynki

72%

Szałowska D. , Bąk-Romaniszyn L.

Pediatria i Medycyna Rodzinna

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2012

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tom 8

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nr 3

258-262

EN

The acute pancreatitis occurs in children much less often than in adults. Though, it usually has a sudden onset and, at the beginning, it proceeds with intensified pain in the area of the abdomen, thanks to the proper diagnostics and treatment, it seldom leads to permanent damage of the pancreas. The most common causes of pancreatitis, apart from calculosis of the gallbladder and other pathological conditions of bile ducts, are the injuries of the abdomen as well as various infectious factors. Acute biliary pancreatitis in children occurs rather seldom, since calculosis of the biliary ducts is not often observed at this age group. Cholesterolic calculosis of the biliary ducts in children most often concerns obese, adolescent girls. Gall stones, usually, consist of a mixture of cholesterol, bile pigments, calcium as well as inorganic substances. A case of a 15.5‑year‑old girl who was hospitalised in our Clinic is presented below. The girl suffered from the pain in the abdomen. On admission, she was initially diagnosed the acute pancreatitis. The results of the laboratory tests (higher activity of amylase in serum and urine, higher activity of lipase, and increased concentration of transaminase) and the ultrasonography of the abdomen let the doctors definitively diagnose the acute biliary pancreatitis. A strict diet, intravenous irrigation, proton‑pomp inhibitors, administration of painkillers and antibiotics led to the improvement of the general state of the patient and normalization of the laboratory tests results. In the tenth day of hospitalization the planned endoscopic regressive cholangiopancreatography (ERCP) with papillotomy was performed. No deposits in bile ducts were revealed. The girl was recommended the procedure of cholecystectomy, which was carried out in due course without any complications.

PL

Ostre zapalenie trzustki występuje u dzieci znacznie rzadziej niż u dorosłych i choć ma zwykle nagły początek i przebiega początkowo z nasilonymi dolegliwościami bólowymi ze strony jamy brzusznej, to dzięki właściwej diagnostyce i leczeniu rzadko prowadzi do trwałego uszkodzenia trzustki. Do najczęstszych przyczyn ostrego zapalenia trzustki u dzieci, oprócz kamicy pęcherzyka żółciowego i innych patologii dróg żółciowych, należą urazy brzucha (zwłaszcza tzw. rowerowy) i czynniki infekcyjne. Ostre żółciopochodne zapalenie trzustki występuje u dzieci sporadycznie, ponieważ nieczęsto zdarza się w tej grupie wiekowej kamica dróg żółciowych. Cholesterolowa kamica żółciowa u dzieci zazwyczaj dotyczy otyłych, dorastających dziewcząt. Na ogół kamienie żółciowe składają się z mieszaniny cholesterolu, barwników żółciowych, związków wapnia oraz substancji nieorganicznych. Prezentujemy przypadek 15,5‑letniej dziewczynki hospitalizowanej w naszej Klinice z powodu bólów brzucha, u której rozpoznaliśmy ostre zapalenie trzustki. Wyniki badań dodatkowych (podwyższona aktywność amylazy i lipazy w surowicy, amylazy w moczu, transaminaz) i wynik badania ultrasonograficznego jamy brzusznej nasunęły podejrzenie żółciopochodnego ostrego zapalenia trzustki. Stosując dietę ścisłą, nawadnianie pozajelitowe, inhibitory pompy protonowej, leki przeciwbólowe, antybiotykoterapię, uzyskano poprawę stanu ogólnego dziecka i normalizację wyników badań dodatkowych. W dziesiątej dobie hospitalizacji wykonano planowo zabieg endoskopowej cholangiopankreatografii wstecznej (ECPW) z papilotomią, nie wykazano obecności złogów w drogach żółciowych. Dziewczynkę zakwalifikowano do zabiegu cholecystektomii, który odbył się w trybie planowym, bez powikłań.

15

Physical factors affecting the production of amylase from Bacillus species isolated from natural environment

72%

Grata K. , Nabrdalik M.

Ecological Chemistry and Engineering. A

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2014

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tom Vol. 21, nr 1

51--60

EN

The influence of different factors on amylolytic activity Bacillus cereus (2 strains) and Bacillus mycoides (2 strains), isolated from soil samples and water of Turawa Lake has been studied. Effect of physiological (pH of reaction mixture in the range of 4.0–8.0, temperature of reaction from 30 to 60 oC) and nutritional parameters (the carbon sources and they concentration in medium) were examined spectophotometricaly for their effect on amylase production. The results obtained showed, that in view of all studied factors the least favorable value of pH was 5.0, while in most cases the most favourable for the process of amylase production were following values 7.0 and 8.0. Moreover, it has been stated that the lowest amount of amylase was noted at 30 oC, and the highest at 60 oC. In case of amylolytic activity, in view of all temperatures the most effective strain was B. mycoides A134 whereas the least B. mycoides G3. The best results of amylase production have been achieved for all remaining strains in two cases – on maltose and glucose medium at all tested temperatures. Additionally, soil occurring strains preferred lower concentration of potato starch (ie 1 %) when compared with water strains which favoured higher concentration (5 %).

PL

Celem badań była ocena wpływu różnych czynników na aktywność amylolityczną Bacillus cereus (2 szczepy) i Bacillus mycoides (2 szczepy) wyizolowanych z gleby i Jeziora Turawskiego. Metodą spektrofotometryczną badano wpływ pH mieszaniny reakcyjnej w zakresie od 5.0 do 8.0 i temperatury w zakresie od 30 oC do 60 oC oraz źródła węgla i jego koncentracji w podłożu na poziom produkowanych amylaz. Stwierdzono, iż najgorszą wartością pH do syntezy amylaz było 5.0, natomiast najlepszą, w większości przypadków pH 7.0. Uwzględniając zakres badanych temperatur, najwyższą aktywność uzyskano w temp. 60 oC, natomiast najniższą w temp. 30 oC dla wszystkich testowanych szczepów. Najbardziej aktywnym szczepem był B. mycoides A 134, natomiast najmniej B. mycoides G3.

16

Effectiveness of hemoperfusion in treatment of patients with non-biliary moderately severe pancreatitis

72%

Oliynyk O. , Slifirczyk A. , Pereviznyk B. , Kushnarov W.

Health Problems of Civilization

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2018

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tom 12

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nr 3

EN

Background. Acute pancreatitis morbidity has been rising annually all around the world. In case of acute necrotic pancreatitis, the mortality reaches 40% in the majority of hospitals. The therapy is effective if the efferent methods involve complex therapy of this disease. One such method is hemoperfusion, i.e. extracorporeal blood purification, which is widely used in toxicology. The authors of the article used hemoperfusion in a complex therapy in patients with severe acute pancreatitis. The following research presents an evaluation of the results of hemoperfusion used in a complex therapy of patients with acute pancreatitis. Material and methods. The study involved 38 patients with acute non-biliary moderately severe pancreatitis who were treated at an intensive care unit of Ternopil University Hospital in Ukraine. 18 patients were treated following the protocol for treatment of acute pancreatitis. In 20 patients, this treatment was additionally combined with hemoperfusion. We determined the levels of amylase, diastase, procalcitonin, bilirubin, malonic dialdehyde, and diene conjugates in blood serum and the level of leukocytes in the blood. Results. The levels of procalcitonin, amylase, bilirubin, leukocytes number, malonic dialdehyde, and diene conjugates were stabilised in patients of both groups, but in those who received hemoperfusion demonstrated much better results. These were manifested by significantly better levels of the amylase, bilirubin, creatinine, urea, procalcitonin, malonic dialdehyde, and diene conjugates in patients who received hemoperfusion (p< 0.005) than in the other group undergoing standard drug therapy. Conclusions. Hemoperfusion can be used as an effective method in the complex treatment of patients with acute pancreatitis.

PL

Wprowadzenie. Corocznie na całym świecie obserwuje się w zrost zachorowalności na ostre zapalenie trzustki. W przypadku ostrego martwiczego zapalenia trzustki śmiertelność w większości szpitali osiąga 40%. Terapia jest skuteczna, jeśli metody odprowadzające obejmują kompleksową terapię tej choroby. Jedną z takich metod jest hemoperfuzja, tj. pozaustrojowa hemodializa krwi, szeroko stosowane w toksykologii. Autorzy artykułu zastosowali hemoperfuzję w kompleksowej terapii u pacjentów z ciężkim ostrym zapaleniem trzustki. Poniższe badania przedstawiają ocenę wyników hemoperfuzji stosowanej w kompleksowej terapii pacjentów z ostrym zapaleniem trzustki. Materiał i metody. W badaniu wzięło udział 38 pacjentów z ostrym, niezłośliwym i umiarkowanie ciężkim zapaleniem trzustki; leczonych w oddziale intensywnej opieki medycznej szpitala uniwersyteckiego na Ukrainie. Zgodnie z protokołem leczenia ostrego zapalenia trzustki leczono18 pacjentów. U 20 pacjentów leczenie to połączono dodatkowo z hemoperfuzją. Określono poziom amylazy, diastazy, prokalcytoniny, bilirubiny, dialdehydu malonowego i koniugatów dienowych w surowicy krwi oraz poziom leukocytów we krwi. Wyniki. Poziom prokalcytoniny, amylazy, bilirubiny, liczby leukocytów, dialdehydu malonowego i koniugatów dienowych były stabilizowane u pacjentów z obu grup, ale u tych pacjentów, u których włączono hemoperfuzję, wyniki były zdecydowanie lepsze. Przejawiały się one znacznie wyższym poziomem amylazy, bilirubiny, kreatyniny, mocznika, prokalcytoniny, dialdehydu malonowego i dienowych koniugatów u pacjentów, którym włączono hemoperfuzję hemoperfuzję (p <0,005) niż w drugiej grupie poddawanej standardowej terapii lekowej. Wnioski. Hemoperfuzja może być stosowana jako skuteczna metoda w kompleksowym leczeniu pacjentów z ostrym zapaleniem trzustki.

17

Resistant starch - classification, structure, production

72%

Leszczynski W.

Polish Journal of Food and Nutrition Sciences

|

2004

|

tom 13/54

|

nr Spec.Issue 1

18

Hemagglutinins of Beauveria bassiana strains: the effect of growth conditions on their production

72%

Peczynska-Czoch W. , Wieczorek J. , Urbanczyk M. J.

|

tom 40

|

nr 5-6

19

Effect of nitrogen fertilization on the amylase activities in potato tubers

72%

Mioduszewska H.

Acta Physiologiae Plantarum

|

1994

|

tom 16

|

nr 4

20

Characterization of catalytic properties of Pisum seed beta-amylases

72%

Zimniak-Przybylska Z.

Genetica Polonica

|

1992

|

tom 33

|

nr 3