Biologically active peptides are of particular interest to food science and nutrition because they may act as potential physiological modulators of metabolism. Hidden or inactive in the amino-acid sequence of food proteins, they can be released or activated in vivo during gastrointestinal digestion, or in vitro during enzymatic hydrolysis and food processing. Egg proteins are an important source of these bioactive peptides. In recent years, major egg protein components, ovoalbumin, conalbumin, ovomucin and phosvitin, have also been shown to contain bioactive sequences. Peptides showing antiadhesive, anticancer as well as immunomodulatory activities were found in ovomucin sequence. Immunoregulative peptides were also found in peptic and chymotryptic hydrolysates of ovoalbumin. Ovocinin and ovocinin(2-7) - angiotensin-converting enzyme inhibitory peptides, whose pharmacological activity was observed in micromolar concentrations - were liberated during in vitro proteolysis of ovoalbumin. Furthermore, many egg protein hydrolysates showed antimicrobial and antioxidant activity after proteolysis with different enzymes, and several active peptides were isolated and identified.
Nutritional support is important for optimum health in fish as it provides the building blocks of innate defense mechanisms and thus protection against infectious diseases. In the present study, we determined the influence of β-glucan (Leiber ®Beta-S) delivered with food on the nonspecific humoral defense mechanisms in fingerling carp. The fish weighed 15-17 g. Four tanks in a recirculation system were stocked with 100 fish each. The water temperature was 20-22°C. The fish were fed daily with commercial pellets containing β-glucan (Leiber ®Beta-S) at doses of 100, 200, and 400 mg per kg of pellets. The feed was prepared by the carp protocol used at the Inland Fisheries Institute. The control group was fed glucan-free pellets. On days 14 and 30 of the experiment, blood was drawn from 10 fish from each group and the nonspecific humoral defense mechanism parameters were examined. The results showed that β -glucan (Leiber ®Beta-S) at doses of 100, 200, and 400 mg per kg of pellets increased the myeloperoxidase activity in blood leukocytes and lysozyme activity in the serum. Total protein and immunoglobulin (Ig) levels were also increased in the serum. The optimal stimulating effect was observed following 30 days of application at a dose of 200 mg of β-glucan (Leiber ®Beta-S) on kg of pellets in fingerling carp.
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