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The comparative characteristics of DNAs isolated from transducing bacteriophages of Staphylococcus aureus

100%

Mlynarczyk G. , Mlynarczyk A. , Jagusztyn-Krynicka E. , Zawidzka E. , Sawicka-Grzelak A. , Osowiecki H.

nr 10-12

EXPRESS ANALYSIS OF CHITOSAN AND ITS DERIVATIVES BY GEL ELECTROPHORESIS

100%

Konovalova M. , Shagdarova B. T. , Zubov V. , Svirshchevskaya E.

tom 24

84 - 95

Chitosan is one of the most promising polymers for biomedical applications due to its unique properties, such as its biocompatibility, low toxicity, biodegradation, and the presence of reactive amino and hydroxyl groups. Analysis of physicochemical properties of chitosan and its derivatives is a time-consuming process and requires expensive equipment and large amounts of the sample. This paper proposes a method for express analysis of the molecular weight (MW), the degree of deacetylation (DD), the substitution degree (SD), and the charge of chitosan and its derivatives using agarose gel electrophoresis under acidic and neutral conditions with Coomassie staining. Positively charged chitosan samples required acidic Tris Acetate-EDTA (TAE) buffer to move in the gel. The electrophoretic mobility of chitosan depended on MW, DD, SD, and the chitosan charge. Based on the dependences obtained by the proposed method, the MW and DD of commercial chitosan samples were determined. Express analysis of chitosan and its derivatives in agarose gel can be used to monitor the reactions of chitosan modification and to analyse samples with unknown characteristics.

Identification of plasmids in Pseudomonas aeruginosa using agarose gel electrophoresis

86%

Wolska K. , Bukowski K.

Polish Journal of Veterinary Sciences

2001

tom 04

nr 2

Structure of the c-Ha-ras-1 locus in human breast tumors

72%

Mackiewicz M. , Choina B. , Kadlof A. , Szala s.

nr 10-12

Expression of the LH-CG receptor gene in various rat tissues