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EN
Background: The effect of sleeping positions during acclimatization to high altitude on Acute Mountain Sickness (AMS) and High Altitude Pulmonary Edema (HAPE) is unknown. We tested whether sleeping with the upper body raised by 5° reduces prevalence and severity of symptoms of AMS as well as of elevated pulmonary artery systolic pressure (PASP) values as a risk factor of HAPE. Methods: Randomly assigning trekking tourist volunteers n = 44 (25 m, 19 f; mean age 42.9 yr) sleeping at 4280 m or 5170 m to the experimental group (upper body elevated by 5°), or to the control group. After exclusion of other reasons for AMS-like symptoms those assumed to be related to AMS were rated by Lake Louise Score questionnaire in the evening and the following morning of the study. Transthoracic echocardiography was performed on both occasions to estimate PASP. Results: In the study group, symptoms of AMS were significantly reduced in younger subjects (p = 0.021), prevalence of AMS was reduced in women (p = 0.156), and PASP values were significantly reduced in older subjects and men (p = 0.032; p = 0.031 respectively). Conclusion: Results suggest that sleeping with the upper body in elevated position during a high altitude ascent may benefit those suffering from AMS or at risk of HAPE due to elevated PASP values.
EN
Introduction: Normobaric hypoxic training (NHT) for preacclimatization at home has found its way into commercial expedition mountaineering. Portable NH-generators produce a normobaric hypoxic gas mixture that can be inhaled using breathing masks at rest or during exercise or it can be pumped into lightweight tents for sleeping. These devices can be rented from commercial companies.Materials and methods: Prior to an expedition to Manaslu (8163 m) NHT was used for preacclimatization over a period of 10 days. Our regimen involved a greater increase in sleeping altitude (10 nights to reach 5400 m) than recommended by the rental company (30 nights to reach 3900 m). Results: No incidents occurred during NHT. Our regimen induced sufficient acclimatization to Manaslu Base Camp (4900 m), as evidenced by the fact that none of the expedition members suffered from AMS although they reached base camp within 2 days from Kathmandu – by helicopter flight to 3500 m and a one day walk the next day. Eight days after leaving Kathmandu, camp III was reached at 6850 m.Conclusions: At natural altitudes above 2500 m an increase of sleeping altitude should not exceed 300-500 m per day. Below 2500 m, there are no restrictions. It is therefore unclear why the company recommends such a slow increase in sleeping altitude. In our opinion, it is not necessary for healthy persons to start with a sleeping altitude below 2500 m. In contrast to natural altitudes, NH exposure can be instantly stopped if symptoms occur. Therefore, it seems safe to expose healthy persons to more liberal normobarichypoxic conditions.
EN
The aim of the experiment was to determine suitable substrate type and optimal plant size for transfer of plantlets from in vitro to ex vitro under experimental outdoor conditions. Tests focused on the effect of substrate type (muddy and sandy) and starting size of plantlets gained through in vitro seed germination (0-3, 3.1-5,5.1-6, 6.1-10 cm) on plant growth. Three parameters (fresh weight, length, and the number of leaves) were compared to evaluate growth. Basic water parameters in experimental water tanks were regularly measured (pH, temperature, electrical conductivity, shadow intensity) and controlled to reach similar conditions to those in the natural habitat of this species. Overwintering was studied in a cellar with newly defined size categories (<6, 6.1-8, 8.1-10, 10.1-12, 12.1-15 cm). Both substrate type and starting size of plantlets significantly impacted growth. Plantlets grew better in the muddy substrate while a 100% success rate of rooting was gained with a starting size of 6.1-10 cm in both substrates. The biggest increase in fresh weight was observed with a starting size of 3.1-5 cm and 5.1-6 cm in both substrates. The greatest increase in fresh weight was observed in plants with a starting size of 3.1-5 cm in the muddy substrate (more than 95% increase). The best overwintering results were gained in the 6.1-8 cm size category.
EN
Seasonal variations of environmental factors, such as temperature and salinity, require metabolic acclimatization in sedentary benthic fauna distributed over a wide geographical range. The soft-shell clam Mya arenaria inhabits the coastal waters of the North Atlantic including North America and Europe. In Europe, M. arenaria populations are distributed from Iceland to the Mediterranean Sea, including the North Sea, the Baltic Sea and the Black Sea. Seasonal changes in physiological parameters (gonad index, condition index, biochemical composition and respiration rate) of M. arenaria from the Baltic Sea (the Gulf of Gdańsk, Poland), and the North Sea (Versee Meer, the Netherlands) were studied. The sex ratio of both populations did not differ from 1:1 and the seasonal gonad index was higher in the Baltic population. The average condition index changed seasonally at both studied sites, and was also higher in the Baltic population (except the autumn) compared to the North Sea. In both studied populations, the content of proteins, lipids and carbohydrates in the soft tissue followed the seasonal variations, and it was higher in the Baltic population. The respiration rate was lower in the Baltic population, and seasonal changes in the respiration rate seem to be correlated with changes in the water temperature. Based on the results obtained in the present study, we suggest that Mya arenaria is characterized by a large phenotypic plasticity and differences in the observed physiological traits are due to acclimatization to ambient environmental conditions.
EN
Shoot tips of Tibouchina urvilleana Cogn. were cultured 4 weeks in vitro in modified Murashige and Skoog (MS) [1962] medium supplemented with growth retardants: paclobutrazol – 0.1, 0.5, 1.0, 5.0 mg·dm⁻³, flurprimidol – 0.1, 1.0, 5.0 mg·dm⁻³, chlorocholine chloride (CCC) – 5.0, 50.0, 250.0 mg·dm⁻³. Explants cultured on the medium without growth substances were used as a control. Rooted microcuttings were transferred to the greenhouse and transplanted into a mixture of 1 peat : 1 perlite, where they were grown for 5 weeks. Plants were then cultivated in a peat substrate during another 5 weeks. Acclimatization of rooted shoots in the greenhouse was affective in 92.5–100%. The survival of plants was lowest when microcuttings were previously cultured on medium with flurprimidol at 5.0 mg·dm⁻³. Cultivation of Tibouchina urvilleana shoots in vitro on media with various growth retardants had a significant effect on the further growth of plants ex vitro. Paclobutrazol and flurprimidol at 5.0 mg·dm⁻³ inhibited very strong growth of plants after 10 weeks of growth ex vitro.
PL
Wierzchołki pędów Tibouchina urvilleana Cogn. prowadzono 4 tygodnie in vitro na zmodyfikowanej pożywce Murashige i Skooga (MS) [1962] z dodatkiem retardantów wzrostu: paklobutrazol – 0,1; 0,5; 1,0; 5,0 mg·dm⁻³, fluropirimidol – 0,1; 1,0; 5,0 mg·dm⁻³, chlorek chlorocholiny (CCC) – 5,0; 50,0; 250,0 mg·dm⁻³. Kontrolę stanowiła pożywka nie zawierająca retardantów wzrostu. Ukorzenione pędy przenoszono do szklarni i sadzono do mieszaniny torfu i perlitu (1:1), gdzie rosły przez 5 tygodni. Następnie rośliny przesadzono do substratu torfowego i uprawiano przez kolejne 5 tygodni. Rośliny przyjęły się w 92,5–100,0%. Najniższy procent przyjęć wykazywały rośliny pochodzące z pożywki zawierającej 5,0 mg·dm⁻³ fluropirimidolu. Wzrost in vitro pędów Tibouchina urvilleana na pożywce zawierającej retardanty wzrostu miał istotny wpływ na dalszy wzrost roślin w podłożu. Paklobutrazol i fluropirimidol w stężeniu 5,0 mg·dm⁻³ bardzo silnie hamowały wzrost roślin ex vitro po 10 tygodniach.
EN
Dirofilariosis caused by the Dirofilaria repens nematodes is widely dispersed in southern Europe, Asia and Africa among dogs, cats, other carnivores and occasionally, humans. The first case of D. repens infection, found in Poland in 2007, concerned humans. In 2009, dirofilariosis was first registered in dogs in 3 focuses in central Poland, Warsaw, Pruszków and Żyrardów, whose range grew considerably with the subsequent identification of D. repens microfilariae in 119 dogs in Warsaw and 18 districts of the Mazowieckie Province. The microfilariae of D. repens were found in blood samples taken from 1588 dogs from all 16 provinces of Poland. D. repens was also recently detected in a mixture of Culex pipiens and Aedes vexans mosquitoes collected in Mazowieckie Province using Real Time PCR. The results of this study confirms the acclimatisation of D. repens on the territory of Poland and a possibility for it to close its life cycle in domestic species of mosquito.
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EN
Background: Trekking in Nepal is popular and generally safe. However preventable trekking injuries and deaths are often the result of poor risk managementand lack of skills – i.e. acute mountain sickness (AMS) and specific First Aid (FA). Shlim and Gallie’s reviews in 1992 and 2004 of trekking deaths here found a fivefold higher risk for fatal outcomes in organized trekking tours (OT) compared to independently organised trekkers (IT), including preventable deaths (i.e. AMS). Our survey sought to identify the AMS, FA and risk management knowledge/skills of OT and IT whilst trekking in Nepal. Methods: Based on previous pilot studies at the Khumbu region in Nepal, and on risk management information provided by leading German trekking operators, a survey was conducted using two questionnaires: one each for OT and IT.The study site was Manang (3,500m) in Annapurna region, Nepal. All trekkers passing through this site who volunteered their participation were included. For evaluation descriptive statistical methods and non-parametric tests were used. Results: 442 questionnaires were completed (155 OT; 287 IT). Mean agewas 36.4 +/– 12.0y and 61% were male. Mountaineering experience was low and FA training was scarce in both groups. IT (92.7%) followed acclimatization recommendations and 59.2% carried AMS medication, compared respectively to OT at 63.2% and 25.2%. Only 27.1% of OT had repatriation plans for a medical emergency. Conclusions: Most of OT and IT were inexperienced in mountaineering and in mountain emergencies. The preparedness of OT overall was inadequate and over-reliant on the organisers whose skills may also be inadequate when responding to an emergency. Responsible trekking should require that more than one person in a group have adequate, up-to-date riskassessment/management and FA skills specific to the trek, and in particular an understanding of altitude profile, AMS, and individual abilities.
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