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Effects of semen collection interval on fresh and frozen semen parameters in Tushin rams

100%

Ari U. , Lehimcioglu N. , Yildiz S. , Kulaksiz R. , Ozturkler Y.

Bulletin of the Veterinary Institute in Puławy

2011

tom 55

nr 1

Ejaculates from three rams were collected with artificial vagina at one day (OD1) and four day intervals (FDsI). Part of the semen samples were pooled, diluted with skim milk based extender: -egg yolk (10%) -glycerol (5%), loaded into 0.25 ml straws, equilibrated, frozen, and afterwards stored in liquid nitrogen. The following spermatozoon parameters were determined in fresh and frozen-thawed semen: motility, viability, abnormality, abnormal acrosome, and membrane integrity. Semen collected at ODI had significantly higher freeze-ability compared with semen collected at FDsI for spermatozoon motility. Frozen-thawed semen collected at ODI had higher viability and membrane integrity, and lower abnormality and abnormal acrosome percentages compared with FDsI. It was concluded that in order to use cryopreservation of the Tushin ram semen, ODI instead of FDsI collection should be preferred.

Effects of different intravaginal progesterone releasing devices on estrous synchronization and LH surge in fat-tailed ewes during non-breeding season

100%

Gungor O. , Cenesiz M. , Pancarci S. M. , Yildiz S. , Kaya M. , Kacar C. , Ozyurtlu N. , Gurbulak K.

tom 63

nr 11

1316-1319

The aims of this study were to compare two methods of estrus synchronization and to evaluate the effectiveness of the PMSG treatment combined with P4 application. Fifty non-lactating seasonal anestrus fat-tailed ewes were randomly assigned into five groups. The controlled internal drug release devices (CIDR) were applied during day 14 in group I and in group II. Progesterone impregnated sponges were applied during day 14 in group III and in group IV. And then 500 IU PMSG was injected in group I and III i.m. intravaginal devices removed. Ewes in group V served as controls. There was no difference between the groups in the peak value of LH and LH surge. Although LH surge was seen in the control groups 5 sheep, none of the control ewes expressed estrus. Different progestagen treatments have no different results when they are evaluated in terms of the success of the estrus synchronization. PMSG application, after P4 treatment, increased the success of the synchronization.