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Cloned cDNA of the pPAG genes (porcine Pregnancy-Associated Glycoprotein) as useful templates for proteomical and genomical diagnoses

100%

Panasiewicz G. , Szafranska B.

Biotechnologia

2005

nr 1

47-60

The PAG (Pregnancy-Associated Glycoproteins) gene family and chorionic expressions of their products suggest an important role(s) of this glycoprotein family throughout pregnancy in various mammals (pig, cattle, sheep, goat, hourse, zebra, cat and mouse). Each cloned cDNA is a major source of template for recombinant PAG protein productions, necessitated for requirements at various diagnostic tests of early pregnancy in ruminants. Moreover, each cDNA may be applied as template for preparation of selective genetic tests (i.e. micro-arrays), can provide essential background for estimation of correlations between various PAG genotypes and physiological reproductivity of each animal. Aforementioned genetic tests presumably will allow for pre-selection of young indyviduals with effective reproductive performances.

Expression on interferon genes in trophoblast; its impact on pregnancy

100%

La Bonnardiere C.

Biotechnologia

1996

nr 2

52-67

In mammals, succesful implantation of semi-allogenic conceptus (embryo +trophoblast) in the maternal uterus is still an enigma.Its understanding will require better knowledge of the complex hormonal and immunological "cross-talk" between implanting conceptus and mother.IN several ungulate species, around the time of implantation, the trophoblast synthesizez and secrets interferons (IFNs), known as antiviral and immunomodualtory cytokines.THe IFN induction is transient (6-7 days long)), and reaches high levels in ruminants and pigs.This IFN synthesis in early pregnancy addresses two man questions:1)Is there a specific IFN gene regulation in trophoblast, and what are the molecular determinants of this specificity (coding and regulatory sequences) ? 2)What is (are) the effect(s) ogf IFN synthesis on the physiological and/or immune maternal response ? This area of research might find applications in animal breeding in particulatr through improvement of embryo transfer and increase in fecundity, in several animal species.