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RNA recombination in the virus-transgenic plant system ? the risk of creating a new virus

100%

Urbanowicz A. , Alejska M. , Figlerowicz M. , Bujarski J. J.

2003

nr 2

129-140

It is generally assumed that RNA recombination is one of the major driving forces in the evolution of plant viruses. This process leads to rearrangements of viral genomes and plays an important role in adaptation, genome repair and genetic variability of RNA viruses. It has been observed that viruses could recombine not only with each other, but also with mRNA of transgenic plants expressing viral genes. This observation has given rise to new concerns about creating virus-resistant transgenic plants, because the recombination could generate the viruses with new properties that were different from the parental strains. In this article, we present the current state of knowledge about recombination between transgens and challenging virus, we discuss what may happen in a field during interaction between the virus and the transgenic plant, and we propose strategies that allow to control the virus-transgene crossovers.

The effect of hFVIII transgene on the chromosomal aneuploidy rate in rabbits

88%

Curlej J. , Parkanyi V. , Bulla J. , Jurcik R. , Chrenek P.

nr 3-4

161-164

The aim of this study was to compare the chromosomal aneuploidy rate between transgenic and non-transgenic rabbits derived from the F4 generation. Chromosomal analysis was carried out on bone marrow samples of New Zealand White transgenic (carrying human factor VIII gene) and non-transgenic rabbits (F4 generation) each having a different genetic background (female no. 1-3-5 line I and female no.1-9-7 line II). C-metaphase plates were obtained from the bone marrow lymphocytes synchronized by the addition of 0.25 mug/ml colcemide. No significant difference in chromosomal aneuploidy between transgenic (61%) and non-transgenic (51.27%) rabbits of line I was observed. A higher but non-significant aneuploidy rate between transgenic and non-transgenic rabbits was found in line II, on the other hand a significant difference (P<0.05) was observed in diploidy rate. In conclusion, chromosomal aneuploidy rates in this experiment were higher than published previously in other reports.