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1
Content available remote In silico study of the structure and function of Streptococcus mutans plasmidic proteins
100%
Caprari S. , Minervini G. , Brandi V. , Polticelli F.
Bio-Algorithms and Med-Systems
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2017
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tom Vol. 13, no. 2
51--61
EN
The Gram-positive bacterium Streptococcus mutans is the principal causative agent of human tooth decay, an oral disease that affects the majority of the world’s population. Although the complete S. mutans genome is known, approximately 700 proteins are still annotated as hypothetical proteins, as no threedimensional structure or homology with known proteins exists for them. Thus, the significant portion of genomic sequences coding for unknown-function proteins makes the knowledge of pathogenicity and survival mechanisms of S. mutans still incomplete. Plasmids are found in virtually every species of Streptococcus, and some of these mediate resistance to antibiotics and pathogenesis. However, there are strains of S. mutans that contain plasmids, such as LM7 and UA140, to which no function has been assigned yet. In this work, we describe an in silico study of the structure and function of all the S. mutans proteins encoded by pLM7 and pUA140 plasmids to gain insight into their biological function. A combination of different structural bioinformatics methodologies led to the identification of plasmidic proteins potentially required for the bacterial survival and pathogenicity. The structural information obtained on these proteins can be used to select novel targets for the design of innovative therapeutic agents towards S. mutans.
2
Growth curve of Streptococcus oralis
86%
Palchaudhuri S. , Palchaudhuri A. , Chatterjee B.
European Journal of Biological Research
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2016
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tom 06
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nr 1
3
Content available remote HPLC-DAD method validation for quantification of dehydroabietic acid and abietic acid in oral spray containing Pinus merkusii heartwood extract and its antibacterial effects on clinically isolated Streptococcus mutant
86%
Sakunpak A. , Saingam W.
Acta Chromatographica
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2024
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tom Vol. 36, no. 3
228--236
EN
Pinus merkusii Jungh & De Vries. has become increasingly gathered more attention from researchers because the plant has a range of folk medicinal uses. Heartwood plant is the major source of dehydroabietic acid (DHAA) and abietic acid (AA), which possesses several medicinal properties, such as antiviral, antimicrobial, antiobesity and anti-inflammatory. The research proposed herein a low-cost, fast, specific, uncomplicated, sensitive, precise reverse-phase high-performance liquid chromatography (RP-HPLC). This method was conducted and validated for evaluating an amount of DHAA and AA in ethanol extract and oral spray containing P. merkusii heartwood extract. Additionally, stability and antimicrobial activities against clinically isolated Streptococcus mutans of the oral spray were determined. The separation was achieved on Pursuit 200Å PFP column, 150 3 4.6 mm, particles of 3 μm with a flow rate of 1.0 mL min1 . Methanol and water (70:30 v/v) were used as eluent with an isocratic mode and sample analysis volume was set at 10 μL, at a detection wavelength of 210 and 245 nm. The developed HPLC method for analysis of DHAA and AA showed good linearity with correlation coefficients equal to 1. Moreover, other validation parameters, comprised of accuracy, precision, specificity and detection and quantitation limits of this method displayed excellent reliability, validity and sensitivity. This method could be an interesting alternative for quantitative measurement of P. merkusii heartwood extract, oral spray formulation and other P. merkusii heartwood extract preparations. The result from antibacterial tests suggested that the oral spray containing P. merkusii heartwood extract is able to inhibit the oral pathogens causing dental caries. The oral spray decreased S. mutans population size by about 0.5–2 Log CFU mL1 at 1–4 h and complete elimination of all bacteria strains within 24 h. This study provides validity for using P. merkusii heartwood extract as an alternative for preventing and treating oral infectious diseases.
4
Microbiology of dental caries
86%
Karpinski T. M. , Szkaradkiewicz A. K.
Journal of Biology and Earth Sciences
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2013
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tom 03
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nr 1
5
Rola bakterii Streptococcus mutans w patogenezie prochnicy
86%
Zandecka-Dziubak J. , Tomaszewska M. , Lipinski D. , Mrozikiewicz P. M.
Herba Polonica
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2004
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tom 50
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nr 2
94-110
PL
W Polsce i na świecie próchnica została uznana za chorobę społeczną. Badania z udziałem ludzi potwierdziły, że za inicjację i postępowanie tej choroby odpowiedzialne są bakterie Streptococcus mutans. Wiele analiz wskazuje na kluczową rolę specyficznych białek S. mutans (antygen 1/11, glukozylotrasferaza GTF, białka GBP) w patogenezie próchnicy. Badania dowiodły, że antygeny te bądź ich fragmenty mogą być efektywnymi immunogenami przeciwko próchnicy.
EN
Dental caries was recognized as a social problem in Poland and in the world. Streptoccocus mutans and Streptococcus sobrinus were strongly implicated as the causative organisms of human and animal dental caries. Specific proteins of S. mutans (antigen l/ll and glucosyltransferase GTF, GBP-proteins) have been implicated as the virulence factors in the molecular pathogenesis of dental caries. A lot of investigations indicated that the antigens or its fragments can be effective immunogenes against caries.
6
Zwiazek miedzy liczba granulocytow obojetnochlonnych w jamie ustnej a liczebnoscia niektorych grup bakterii
72%
Zarzycka B. , Pluciennik M. , Krzeminski Z.
Medycyna Doświadczalna i Mikrobiologia
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2005
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tom 57
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nr 2
225-229
PL
Celem pracy było zbadanie, czy istnieją zależności między liczbą granulocy- tów obojętnochłonnych znajdujących się w jamie ustnej a liczbą niektórych bytujących tu bakterii (paciorkowców, pałeczek hemofilnych, pałeczek kwasu mlekowego, pałeczek jelitowych, gronkowców i Streptococcus mutans). Ustalono, że poza pałeczkami jelitowymi, liczebność badanych grup bakterii statystycznie znamiennie ujemnie koreluje z liczbą neutrofili.
EN
The aim of the study was to evaluate the relationship between the number of polymorphonuclear leukocytes (PMNL) and the number of selected bacteria in human oral cavity. Sixty one healthy people (periodontal index of Russel's did not exceed 0.2), aged 22-26 were investigated. PMNL were isolated by rinsing oral cavity with isotonic, buffered NaCl solution. Nonstimulated whole saliva was bacteriologically examined. The number of PMNL, which was obtained from mouth of examined people was between 100000 and 4200000 in 100 mL of rinsings. Usually, i.e. in 34% of cases, the number was between 500000-1000000. Streptococci were isolated from all tested people and their number was the biggest. Almost in all cases there were isolated cariogenic bacteria - Streptococcus mutans (95%) and lactobacilli (93%). Haemophili, staphylococci, including Staphylococcus aureus, were found relatively often (in 86%, 86% and 52% respectively). Gram-negative enteric rods were rarely isolated (33%) and were the least numerous group of all. It has been stated, that there is a comparatively strong negative correlation between the number of PMNL in oral cavity and the number of streptococci (r=-0.55; p<0.0001), haemophili (r=-0.564; p<0.0001), staphylococci (r=-0.538; p<0.0001), lactobacilli (r=-0,407; p=0.0017) and Streptococcus mutans (r=-0.483; p=0.0002). The results suggest that PMNL are one of the factors controlling the number of some bacteria in human oral cavity.
7
Genetyka czynnikow wirulencji paciorkowcow jamy ustnej
58%
Wolinowska R.
Postępy Mikrobiologii
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1993
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tom 32
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nr 4
253-270
8
Content available remote The influence of silver nanoparticles introduced into RTV-silicone matrix on the activity against Streptococcus mutans
58%
Chladek G. , Mertas A. , Krawczyk C. , Stencel R. , Jabłońska-Stencel E.
Archives of Materials Science and Engineering
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2016
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tom Vol. 78, nr 2
59--65
EN
Purpose: The silicone based room temperature vulcanized (RTV) polymers are commonly used materials for medicine, especially for dentures and maxillofacial prostheses. Unfortunately, the colonization of those materials by pathogenic microorganisms is wellknown problem related with their applications. The aim of presented study was to examine antibacterial properties of RTV silicone for dentistry modified with silver nanoparticles. Design/methodology/approach: The silver nanoparticles were introduced into twocomponent system silicone based materials. The presence of silver nanoparticles was investigated with scanning electron microscope (SEM). The antibacterial activity against Streptococcus mutans was determined. The result were statistically analysed with a Statistica 12.5 software and non-parametric Kruskal-Wallis test (α = 0.05). Findings: The silver nanoparticles introduction into RTV - silicone allowed to enhance the antimicrobial resistance against standard strain of Streptococcus mutans. Research limitations/implications: In this research only Streptococcus mutans bacterium strain was used. In future activity of presented materials against other pathogenic bacteria living in oral cavity should be determined. Additionally long term investigation should be prepared. Practical implications: The colonization of dental materials with pathogenic bacteria and fungus is one of the most important and still unresolved problems related to exposition on oral environment. The low microbiological resistance of RTV-silicones and antimicrobial potential of silver were reported in numerous studies. The gram-positive Streptococcus mutans is commonly found in the human oral cavity and it is an important factor to tooth decay. Originality/value: The resistance against Streptococcus mutans of modified material was enhanced. The investigated materials could be a potential factor a potential conducive to reducing the risk of oral cavity infections.
9
Szczepionka zamiast wiertła
58%
Socha D. , Chalubinski M.
Wiedza i Życie
|
2009
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nr 07
10
Overexpression of gtfbeta gene fragments from Streptococcus mutans encoding Glu and Cat domains in Escherichia coli
58%
Lipinski D. , Zandecka-Dziubak J. , Juzwa W. , Zeyland J. , Mrozikiewicz P. M. , Slomski R.
Herba Polonica
|
2004
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tom 50
|
nr 2
EN
Streptococcus mutans is involved in the initiation and progression of dental caries. Fragments of a gtfB gene from Streptococcus mutans encoding Glu and Cat peptides of approximately 35 and 45 kDa, respectively, were cloned from this organism and sequenced. Recombinant Glu and Cat peptides were overexpressed in Escherichia coli with C-terminal sequence containing additional six histidine residues and enterokinase recognition site. The recombinant peptides were purified from E. coli by metal affinity chromatography. These proteins will be used to prepare efficient vaccine against dental caries.
PL
Bakterie Streptococcus mutans są główną przyczyną inicjacji i rozwoju próchnicy zębów. Fragmenty genu gtß S. mutans kodujące peptydy Glu i Cat o masie cząsteczkowej 35 i 45 kDa zostały sklonowane i zsekwencjonowane. Rekombinowane peptydy Glu i Cat ulegają nadekspresji w komórkach Escherichia coli. Końce karboksylowe peptydów zostały zmodyfikowane przez dodanie sekwencji kodującej 6 reszt histydyny oraz miejsca rozpoznawanego przez enterokinazę. Rekombinowane peptydy zostały oczyszczone z bakterii E. coli metodą chromatografii powinowactwa. Oba białka zostaną wykorzystane do przygotowania skutecznej szczepionki przeciw próchnicy zębów.
11
Dwuskładnikowe systemy regulacyjne ziarenkowców Gram-dodatnich i ich rola w tworzeniu biofilmu
44%
Nowak A. , Tyski S.
Postępy Mikrobiologii
|
2012
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tom 51
|
nr 4
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