Wyniki wyszukiwania - Biblioteka Nauki

1

Metabolism of conjugated sterols in eggplant. Part 2. Phospholipid : steryl glucoside acyltransferase

100%

Potocka A. , Zimowski J.

Acta Biochimica Polonica

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2008

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tom 55

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nr 1

135-140

EN

A membrane-bound phospholipid : steryl glucoside acyltransferase from Solanum melongena leaves was partially purified and its specificity and molecular as well as kinetic properties were defined. Among the steryl glycosides tested (e.g. typical plant steryl glucosides, steryl galactosides and cholesteryl xyloside) the highest activity was found with cholesteryl glucoside, but some structurally related compounds such as sito- and stigmasteryl glucoside or galactoside as well as cholesteryl galactoside were also acylated, albeit at lower rates. The investigated enzyme was able to use all classes of phosphoglycerolipids (phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol) as an acyl source for biosynthesis of acylated steryl glucoside. Among them 1,2-dimirystoylphosphatidylic acid appeared to be the best acyl donor. Apart from phosphoglycerolipids, 1,2-diacylglycerols were also used as acyl donor for steryl glucoside acylation, although at a distinctly lower rate. The acyl moiety was transferred from the C-1 position of phospholipid molecule. The investigated acyltrasferase activity was stimulated by 2-mercaptoethanol, Triton X-100, 1-monoacylglycerols and inhibited in the presence of divalent cations such as Ca2+, Mn2+, Zn2+ or Co2+, some lipids (MDGD, ceramide), detergents (Tween 20, 40, 60 and 80, Tyloxapol, sodium deoxycholate) and high ionic strength.

2

Metabolism of conjugated sterols in eggplant. Part 1. UDP-glucose : sterol glucosyltransferase

100%

Potocka A. , Zimowski J.

Acta Biochimica Polonica

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2008

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tom 55

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nr 1

127-134

EN

A membrane-bound UDP-glucose : sterol glucosyltransferase from Solanum melongena (eggplant) leaves was partially purified and its specificity as well as molecular and kinetic properties were defined. Among a wide spectrum of 3-OH steroids (i.e. typical plant sterols, androstane, pregnane and cholestane derivatives, steroidal alkaloids and sapogenins) and triterpenic alcohols, the highest activity was found with 22-oxycholesterol. UDP-glucose appeared to be the best sugar donor. The enzyme preparation was also able to utilize UDP-galactose, TDP-glucose and CDP-glucose as a sugar source for sterol glucosylation, however, at distinctly lower rates. The investigated glucosyltrasferase was stimulated by 2-mercaptoethanol, Triton X-100 and negatively charged phospholipids, and inhibited in the presence of UDP, mono-, di- and triacylglycerols, divalent cations such as Zn2+, Co2+, high ionic strength, cholesteryl glucoside, galactoside and xyloside and some amino acid-modifying reagents (SITS, DIDS, PLP, DEPC, pCMBS, NEM, WRK and HNB). Our results suggest that unmodified residues of lysine, tryptophan, cysteine, histidine and dicarboxylic amino acids are essential for full enzymatic activity and indicate that a glutamic (or aspartic) acid residue is necessary for the binding of sugar donor, i.e. UDP-glucose in the active site of the GT-ase while histidine and cysteine residues are both important for the binding of the nucleotide-sugar as well as of the steroidal aglycone.

3

Effect of substrate utilization time on the yield of eggplant [Solanum melongena L.]

100%

Markiewicz B. , Golcz A. , Politycka B.

Roczniki Akademii Rolniczej w Poznaniu. Ogrodnictwo

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2004

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tom 37

4

Enhancement of vegetative parameters of brinjal in proplates by the application of bacterial endophytes- Azospirillum brasilense and Pseudomonas fluorescens

88%

Sivagamasundari U. , Gandhi A.

International Letters of Natural Sciences

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2014

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tom 22

EN

An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from brinjal, in different combinations with inorganic fertilizers by seed inoculation of brinjal to observe preliminary vegetative growth at 15th and 30th day and pigment contents in vegetable nursery bed (proplates). A total number of 28 endophytic bacteria isolated from brinjal from three localities (Annamalai University, Karaikal and Putthur). Further the isolates were subjected to various biochemical tests for their species level identification and nitrogen fixing ability was estimated. Based upon their N-fixing ability and IAA production, two strains, one Azospirillum sp. and one Pseudomonas sp. isolate was selected and tested for its performance in brinjal. The seeds treated with 75% Chemical fertilizer + Azospirillum brasilense + Pseudomonas fluorescens (T6) showed maximum plant vegetative characters, followed by others compared with control.

5

Evidence for essential histidine and dicarboxylic amino-acid residues in the active site of UDP-glucose : solasodine glucosyltransferase from eggplant leaves.

88%

Nawłoka P. , Kalinowska M. , Pączkowski C. , Wojciechowski Z. A.

Acta Biochimica Polonica

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2003

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tom 50

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nr 2

567-572

EN

Effects of several chemical probes selectively modifying various amino-acid residues on the activity of UDP-glucose : solasodine glucosyltransferase from eggplant leaves was studied. It was shown that diethylpyrocarbonate (DEPC), a specific modifier of histidine residues, was strongly inhibitory. However, in the presence of excessive amounts of the enzyme substrates, i.e. either UDP-glucose or solasodine, the inhibitory effect of DEPC was much weaker indicating that histidine (or histidines) are present in the active site of the enzyme. Our results suggest also that unmodified residues of glutamic (or aspartic) acid, lysine, cysteine, tyrosine and tryptophan are necessary for full activity of the enzyme. Reagents modifying serine and arginine residues have no effect on the enzyme activity.

6

Studies on the effect of different insecticidal applications on quality, nutritional parameters and total antioxidant capacity of brinjal (Solanum melongena L.)

75%

Goswami M. R. , Pati U. K. , Maity P. , Mukhopadhyay A.

International Letters of Natural Sciences

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2018

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tom 69

EN

Brinjal (Solanum melongena L.) suffers from the attack of various pests throughout its different stages of the crop growth. Different insecticides are used to control these pest infestations. The application of these insecticides on brinjal plants may have an effect on their quality and nutritional parameters of fruit samples. In the present study effect of three different insecticides viz. Triazophos 40% E.C. Carbaryl 50% W.D.P, Cypermethrin 10% E.C. and Azadiractin 10,000 ppm formulation belonging to different groups namely organophosphate, carbamate, synthetic pyrethroids and botanical insecticide on brinjal fruit quality, nutritional parameters and total antioxidant capacity (TAC) were assessed in field conditions. The results showed that different insecticides at field application dose showed minute insignificant variation in fruit nutritional and total antioxidant activity parameters in comparison to the control samples. The fruit quality parameters also showed minute variation in comparison to the control, except in the case of Triazophos treated samples which showed significant variation (p<0.05) in total soluble sugar content and crude protein.

7

Wielokrotne użytkowanie podłoży w uprawie oberżyny (Solanum melongena L.)

75%

Golcz A. , Politycka B. , Bosiacki M.

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2006

|

nr 1

8

The effect of side shoots pruning on the growth and yielding of eggplant [Solanum melongena L.] in greenhouse production

75%

Cebula S.

|

nr 2

9

The in vitro synthesis of diosgenin mono- and diglucoside by enzyme preparations from Solanum melongena leaves

75%

Paczkowski C. , Wojciechowski Z. A.

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1993

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tom 40

|

nr 1

10

Patogeniczność izolatów Clavibacter michiganensis subsp. sepedonicus względem bakłażana (Solanum melangena) i ziemniaka (Solanum tuberosum)

71%

Pastuszewska T. , Gryn G. , Lisowska M. , Wegierek A.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2010

|

nr 257-258

11

Warto zainteresowac sie uprawa oberzyny

63%

Ambroszczyk M.

Hasło Ogrodnicze

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2000

|

nr 02

50-52

12

Comparison of nutrient content in fruit of commercial cultivars of eggplant (Solanum melongena L.)

63%

Ayaz F. A. , Colak N. , Topuz M. , Tarkowski P. , Jaworek P. , Seiler G. , Inceer H.

Polish Journal of Food and Nutrition Sciences

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2015

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tom 65

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nr 4

EN

Eggplant (Solanum melongena L.) is one of the most popular common major vegetable crops worldwide. This study evaluated the nutritional content of seven commercial eggplant fruits in terms of fatty acid, mineral, sugar, organic acid, amino acid and polyamine contents. The most abundant fatty acid was linoleic acid (range, 39.14-53.81%, ave. 45%), and the most abundant mineral was K (range, 1556.2-3171.6 mg/kg fw, ave. 2331.9). The major organic acid was malic acid (range, 129.87-387.01 mg/g fw, ave. 157.49), and the major sugar was fructose (range, 1242.811379.77 mg/100 g fw, ave. 1350.88). The major polyamine was putrescine (11.54 and 25.70 nmol/g fw, ave. 17.86), and the major amino acid was glutamine (148.4 and 298.75 mg/100 g fw, ave. 219.74). Overall, taking into account the export potential of eggplants, these results may contribute to further studies aiming to improve other nutrient-rich varieties of eggplant in breeding programs.

13

Effect of plant pruning and topping on yielding of eggplant in unheated foil tunnel

63%

Buczkowska H.

Acta Scientiarum Polonorum. Hortorum Cultus

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2010

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tom 09

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nr 3

EN

Due to strong eggplant growth and the formation of large vegetative weight quantities in growing under glass and foil it was deemed useful to conduct studies on the effect of pruning intensity on yielding of this vegetable in rooms. The aim of the foregoing studies was to demonstrate the effect of plant pruning and topping manner on yield quantity and earliness. The experiment was conducted in the years 2001–2003. The study objects were the plants of ‘Epic F1’ cultivar. Eggplant was grown in rigid foil cylinders of the capacity of 10 dm3 in the peat substrates. The plants were trimmed, managing for one, two, three, four, five, six guiding shoots and in the natural form, without trimming. Topping cut was performed in each combination after the first fruit harvest. The experiment was established and conducted as a two-factor one, in accordance with a complete randomization system: A – pruning method (a = 7), B – topped and non-topped plants (b = 2). Each combination of the examined factors was represented by 20 plants (experimental units). A significant effect of pruning intensity on the quantity and earliness yielding was demonstrated. The highest marketable fruit yield was obtained from plants managed for two (3.82 kg.m-2), three (3.98 kg.m-2), and four (3.87 kg.m-2) guiding shoots. Managing for one guiding shoot significantly decreased the total and marketable fruit number. The highest early yield was collected from plants managed for one and two guiding shoots. A single topping cut performed after the first fruit harvest did not affect marketable yield and marketable fruit number.

PL

Z uwagi na silny wzrost roślin oberżyny i tworzenie dużej ilości masy wegetatywnej w uprawie pod szkłem i folią za potrzebne uznano badania nad wpływem intensywności cięcia na plonowanie tego warzywa w pomieszczeniach. Celem niniejszych badań było wykazanie wpływu sposobu cięcia oraz ogławiania roślin na wielkość i wczesność plonu. Doświadczenie prowadzono w latach 2001–2003. Obiekt badań stanowiły rośliny odmiany ‘Epic F1’. Oberżynę uprawiano w cylindrach z folii sztywnej, o pojemności 10 dm3 w substracie torfowym. Rośliny cięto prowadząc na jeden dwa, trzy, cztery, pięć, sześć pędów przewodnich oraz w formie naturalnej bez cięcia. Cięcie ogławiające wykonywano w każdej kombinacji po pierwszym zbiorze owoców. Doświadczenie założono i przeprowadzono jako dwuczynnikowe według układu kompletnej randomizacji: A – metoda cięcia (a = 7), B – rośliny ogławiane i nieogławiane (b = 2). Każda kombinacja badanych czynników reprezentowana była przez 20 roślin (jednostek eksperymentalnych). Wykazano istotny wpływ intensywności cięcia na wielkość i wczesność plonowania. Największy plon handlowy owoców otrzymano z roślin prowadzonych na dwa (3,82 kg.m-2), trzy (3,98 kg.m-2), cztery (3,87 kg.m-2) pędy przewodnie. Prowadzenie na jeden pęd przewodni znacznie zmniejszyło liczbę owoców ogółem oraz handlowych. Największy plon wczesny zebrano z roślin prowadzonych – na 1 pęd oraz 2 pędy przewodnie. Jednokrotne cięcie ogławiające wykonane po pierwszym zbiorze owoców nie wpłynęło na plon handlowy oraz liczbę owoców handlowych.

14

Effect of plant nutritional status on the yield of eggplant (Solanum melongena L.) grown in organic substrates. Part I. Nitrogen, phosphorus, potassium

63%

Markiewicz B. , Golcz A. , Kujawski P.

Acta Scientiarum Polonorum. Hortorum Cultus

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2008

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tom 07

|

nr 2

EN

In the years 2002–2003, in a foil tunnel, vegetation experiment in the growing of two eggplant cultivars ‘Epic F1’ and ‘Solara F1’ were carried out. Plants were grown in cylinders of 6 dm3 capacity filled with substrate which consisted of: 1) raised peat (from Lithuania), 2) pine bark + low-moor peat (v : v = 1 : 1). In the vegetation period, top-dressing with nitrogen, phosphorus and potassium was applied. Fruit harvest was carried out many times. The total fruit yield was determined. Index parts of plants were sampled for analyses in which the following values were identified: in ‘Epic’ cultivars: 1.12–3.40% N; 0.42–1.14% P; 1.80–4.81% K. In ‘Solara’ cultivars, the following values were found: 1.17–3.50% N; 0.53–1.27% P; 1.96–4.00% K, depending on the substrate and the fertilization level. Differences were found in the total yield and in the nutritional status of plants, depending on the substrate, fertilization level, cultivar and term of sampling.

PL

W latach 2002–2003 w tunelu foliowym przeprowadzono doświadczenia wegetacyjne z uprawą dwóch odmian oberżyny ‘Epic F1’ i ‘Solara F1’. Rośliny uprawiano w cylindrach o objętości 6 dm3 wypełnionych podłożem, które stanowiło: 1) torf wysoki z Litwy, 2) korę sosnową + torf niski (v : v = 1 : 1). W okresie wegetacji stosowano nawożenie pogłówne azotem, fosforem i potasem Wykonywano wielokrotny zbiór owoców. Określono plon ogólny owoców. Pobierano części wskaźnikowe roślin do analiz, w których oznaczono: u odmiany ‘Epic’ 1,12–3,40% N; 0,42–1,14% P; 1,80–4,81% K, natomiast u odmiany ‘Solara’ 1,17– 3,50% N; 0,53–1,27% P; 1,96–4,00% K w zależności od podłoża i poziomu nawożenia. Stwierdzono różnice w plonie ogólnym, oraz w stanie odżywienia roślin w zależności od podłoża, poziomu nawożenia, odmiany i terminu pobrania prób.

15

Effect of nitrogen, phosporus and potassium fertilization on yielding and biological value of fruits of aubergine [Solanum melongena L.]

63%

Markiewicz B. , Golcz A.

Journal of Elementology

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2010

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tom 15

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nr 4

661-669

EN

A plant growing experiment was conducted in 2002-2003 on the aubergine cultivars Epic F1 and Solara F1 grown in an unheated polyethylene tunnel greenhouse at the Experimental Station in Marcelin, the University of Life Sciences in Poznań. Seedlings were planted on May 15 on beds at a 0.5 × 0.5 m spacing, i.e. 4 plants m–2, into 6 dm3 cylinders filled with a mixture, limed to pHH2O = 6.5, of mineral soil (light loamy sand containing 12% clay fraction – deposited on medium-heavy loam) with highmoor peat from Lithuania (v : v = 4 : 1). Basic fertilization – pre-vegetation and top dressing with macronutrients, based on an analysis of the substrate using the universal method in 0.03 M CH3COOH, was determined to attain the assumed levels: L (N – 200, P – 175, K – 330 mg dm–3), S (N – 300, P – 265, K – 500 mg dm–3), H (N – 400, P – 350, K – 665 mg dm–), while maintaining the N : P : K ratio at 1 : 0.9 : 1.7. The aim of this study has been to determine the effect of a fertilization level and cultivar on the yield and biological value of fruits of aubergine grown on a mixture of mineral soil with highmoor peat (v : v – 4 : 1). The total yield, number of fruits and weight of individual fruits were determined. Significant effect was found for the fertilization level and cultivar on the total yield, mean number of fruits and weight of a single aubergine fruit. Fruits of cv. Epic F1 aubergine contained more vitamin C than fruits of cv. Solara F1. In both years, the solids content in fruits of the two aubergine cultivars ranged from 4.0 to 5.5 %. A higher mean dry matter content in aubergine fruits was recorded in cv. Solara F1.

PL

W latach 2002-2003 przeprowadzono doświadczenia wegetacyjne z uprawą oberżyny odm. Epic F1 i Solara F1 w nieogrzewanym tunelu foliowym w Stacji Doświadczalnej Marcelin Uniwersytetu Przyrodniczego w Poznaniu. Rośliny sadzono 15 maja na zagonach w rozstawie 0,5 × 0,5 m, tj. 4 rośliny m–2, w cylindrach o obj. 6 dm3 wypełnionych zwapnowaną do pHH2O = 6,5 mieszaniną gleby mineralnej (piasek gliniasty lekki o zawartości 12% części iłowych – zalegający na glinie średniej) z torfem wysokim z Litwy (v : v = 4 : 1). Nawożenie podstawowe – przedwegetacyjne i pogłówne makroskładnikami, oparte na analizie podłoża wykonanej metodą uniwersalną wg NOWOSIELSKIEGO (1988) w 0,03 M CH3COOH – ustalono do założonych poziomów z zachowaniem proporcji makroskładników N : P : K = 1 : 0,9 : 1,7 : N (N – 200, P – 175, K – 330 mg dm–3), S (N – 300, P – 265, K – 500 mg dm–3), W (N – 400, P – 350, K – 665 mg dm–3). Celem pracy było określenie wpływu poziomów nawożenia na plon i wartość biologiczną owoców oberżyny uprawianej w mieszaninie gleby mineralnej z torfem wysokim (v : v – 4 : 1). Określono plon ogólny owoców, liczbę owoców, średnią masę pojedynczego owocu oraz wartość biologiczną owoców. Stwierdzono istotny wpływ poziomu nawożenia i odmiany na plon ogólny, średnią liczbę owoców oraz masę pojedynczego owocu oberżyny. Owoce oberżyny odmiany Epic F1 zawierały więcej witaminy C niż owoce odmiany Solara F1. We wszystkich latach badań zawartość ekstraktu w owocach obu odmian oberżyny wynosiła od 4,0 do 5,5%. Większą średnią zawartość suchej masy w owocach oberżyny oznaczono u odmiany Solara F1.

16

Wplyw ciecia oglawiajacego na plonowanie oberzyny [Solanum melongena L.] w nieogrzewanym tunelu foliowym

63%

Buczkowska H. , Kowalska G.

|

nr 1

223-227

17

Gruszka miłosna, czyli oberżyna

63%

Rosinska A. , Bralewski T. W.

Hasło Ogrodnicze

|

2010

|

nr 09

18

Wplyw stadium dojrzalosci oraz przechowywania na zawartosc antocyjanow i parametry barwy skorki w owocach oberzyny [Solanum melongena L.]

63%

Gajewski M. , Arasimowicz D. , Gajewska M.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EEE: Horticultura

|

2006

|

tom 16

29-37

19

Oberżyna

63%

Jankowska-Kapica M. , Badowska O.

Kuchnia. Magazyn dla Smakoszy

|

2015

|

nr 08

20

TLC and GLC analysis of individual molecular species in natural mixtures of steryl glycosides isolated from eggplant

63%

Zimowski J. , Wojciechowski Z. A.

|

1996

|

tom 42

|

nr 4

269-272

PL

Opracowano prostą, dwustopniową metodę analizy składu naturalnych frakcji roślinnych glikozydów steroli. Metoda polega na wstępnym rozdzieleniu frakcji, ze względu na rodzaj składnika cukrowego, metodą chromatografii cienkowarstwowej i następnym rozdzieleniu poszczególnych glikozydów, ze względu na rodzaj składnika sterolowego, metodą chromatografii gazowej.

EN

A simple, two-step method for analysis of plant steryl glycosides is described. In the first step steryl glycosides are separated by TLC according to their sugar component. In the second step steryl glycosides are separated by GLC according to their sterol component.