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EN
The structure of epicuticular wax on the stomata surface in both very young and one-year-old fir needles from Risnjak National Park in Croatia were examined with a scanning electron microscope. The stomata surface is first covered with wax crystalloids in the form of rodlets. In the course of time the rodlets become fused and agglomerated into amorphous wax crusts; this process begins very early. In addition to wax crystalloid quantitative estimation indicates a relatively large total amount of amorphous wax in very young needles (~20 days) and a very high percentage of amorphous wax crusts in one-year-old needles. Wax crusts cover stomatal pores in addition to the rims, making normal gas exchange impossible. The early start of wax degradation may be interpreted as a possible result of air pollution.
EN
Chlorophyll and carotenoid content was studied in needles of damaged silver fir (Abies alba Mill.) trees growing in a polluted habitat, Risnjak National Park, and of almost undamaged ones growing at a much less polluted site, Donja Dobra, both locations in the Gorski Kotar region. Chlorophyll a and b, total chlorophylls and carotenoids were lower in needles from the polluted Risnjak site than in those from the unpolluted Donja Dobra site. The data indicate an increase of pigment content over the course of a year; and somewhat smaller content in the first in comparison with the second experimental year. The chlorophyll a:b ratios varied independently of pollution level and locality.
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