Exposure of Rhizopogon roseolus mycelia to 15 mmol·dm⁻³ cadmium for 24 h induces a different pattern of Mn-SOD on polyacrylamide gels, probably being a changed form of an originally existing one. The presence of cadmium affects the chromatographic properties of this enzyme and its mobility through the acrylamide gel. This new isoform was purified using DEAE Trisacryl chromatography. Cadmium induced isoform adsorbed stronger to the ligands and was eluated with a Tris-HCl buffer containing 0.1 mol·dm⁻³ NaCl. SOD from control samples (not treated with cadmium) was eluated with the same buffer without NaCl.
Pinus sylvestris seedlings grown in containers in a peat-vermiculite substrate were inoculated with vegetative inoculum of 11 strains Rhizopogon roseolus (Corda.) Th. M. Fries. Seeds were germinated in May 2006. Five months after germination the seedlings were evaluated for their growth and development and ectomycorrhizal frequency. There were significant differences between trait seedlings P. sylvestris inoculated 11 strains used in this experiments. The highest mean values of height and steam diameter were obtained with seedlings inoculated with strain G fungus R. roseolus. There were no significant differences between dry weight seedlings inoculated all strains. R. roseolus formed ectomycorrhizae on 100% of the inoculated plants at all variants. The highest colonization of short roots were obtained with seedlings inoculated with strain D this fungus (29%). There were destined strains D, G and I fungus R. roseolus to further studies.
Scots pine seedlings inoculated with 11 strains Rhizopogon roseolus were planted out on sylviculture area in March 2007. After 2 and 3 vegetation seasons height, stem diameter, number of buds and lateral shoot, length of the lateral shoots last season and seedlings survival were studied. The highest were seedlings inoculated with strain A, C, D, G, J and K (after 2 and 3 years). However the most thickness were seedlings inoculated with strain J (after 2 year) and D, E, J (after 3 year). Survival of seedlings was higher than 85%, without seedlings inoculated strain H.
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