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EN
The main component of Mediterranean flour moth (Anagasta kühniella Zell) pheromone (9Z,12E)-9,12-tetradecadien-1-ol acetate (ZETA) was obtained in the course of stereoselective Witting reaction of l-acetoxynonane-9-al with ylide generated from (3E)-pentenetriphenylphosphonium bromide in the presence of sodium bis(trimethylsilyl)amide (NaHMDS). Among the three adhesives CUSA, Estonia, Poland), evaluated under laboratory conditions, applied with pheromone ZETA and 2,6-di-tert-butyl-4-methylphenol used as antioxidant on sticky paper stripes, the highest number of male catching revealed the last one. After one fortnight the catching effectiveness of all the glues tested decreased.
EN
Three Chalcididae, namely Brachymeria ceratoniae Delvare sp. nov., Proconura persica sp. nov. and Psilochalcis ceratoniae Delvare sp. nov., are described from Iran. They were all reared from the carob moth Apomyelois ceratoniae (Lepidoptera: Pyralidae), the main pest of Ceratonia siliqua. Invreia subita Nikol'skaya, 1960 is formally transferred to the genus Psilochalcis. A lectotype is designated for Brachymeria confalonierii Masi, 1929.
EN
We report on generation of marker-free ('clean DNA') transgenic rice (Oryza sativa), carrying minimal gene-expression-cassettes of the genes of interest, and evaluation of its resistance to yellow stem borer Scirpophaga incertulas (Lepidoptera: Pyralidae). The transgenic indica rice harbours a translational fusion of 2 different Bacillus thuringiensis (Bt) genes, namely cry1B-1Aa, driven by the green-tissue-specific phosphoenol pyruvate carboxylase (PEPC) promoter. Mature seed-derived calli of an elite indica rice cultivar Pusa Basmati-1 were co-bombarded with gene-expression-cassettes (clean DNA fragments) of the Bt gene and the marker hpt gene, to generate marker-free transgenic rice plants. The clean DNA fragments for bombardment were obtained by restriction digestion and gel extraction. Through biolistic transformation, 67 independent transformants were generated. Transformation frequency reached 3.3%, and 81% of the transgenic plants were co-transformants. Stable integration of the Bt gene was confirmed, and the insert copy number was determined by Southern analysis. Western analysis and ELISA revealed a high level of Bt protein expression in transgenic plants. Progeny analysis confirmed stable inheritance of the Bt gene according to the Mendelian (3:1) ratio. Insect bioassays revealed complete protection of transgenic plants from yellow stem borer infestation. PCR analysis of T₂ progeny plants resulted in the recovery of up to 4% marker-free transgenic rice plants.
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EN
A new genus, Furcata is erected and Furcata quadrangula is described as a new species Jour known species, Furcata dichromella (Ragonot), F. paradichromella (Yamanaka) F.pseudodichromella (Yamanaka) and F. karenkolla (Shibuya) are positioned in the new genus with F. dichromella (Ragonot) as the type species. The first three are transferred from Trachycera Ragonot, the last from Eurhodope Hübner. Both male and female genitalia of these five species, except the female of the new species, are illustrated. The type of the new species and other specimens examined are deposited in the Institute of Zoology, Chinese Academy of Sciences, Beijing (abbreviated as IZCAS in the following text).
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