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The educational publication contains microphotographs and description of histological preparations of the structure of marbled crayfish. The above materials may be used both for carrying out laboratory work on disciplines "Histology", "Cytology", "Cell Biology", "Special Practice", and for self-study of relevant educational topics. Designed for specialists in the field of hydrobiology and histology, students and graduate students of institutions of higher education who studying in the field of "091 Biology", "207 Water bioresources and aquaculture" and "162 Biotechnology and bioengineering". The publication contains the results of studies conducted by President’s of Ukraine grant for competitive projects Ф75/142 «The reproductive potential of invasive species of Dnieper region reservoirs and their impact on bioproductivity formation» (№ 0118U006319) of the State Fund for Fundamental Research.
EN
The results of studies on the effects of low molecular weight acidic solution peptides on the growth and development of the marbled crayfish artificial cultivation.An increasing weights of juvenile freshwater crayfish under the influence of dietary supplement "Albuvir" drug. With the use of histological methods of research, found the impact of 0.01 % solution of the drug on the state of the marbled crayfish lobules of hepatopancreas and fat cells. Developed a method for growing juvenile freshwater crayfish with "Albuvir", which allows to increase the weight gain of crustaceans on 24.3–27.2 % and reduce the level of cannibalism at 20 %.
EN
The article shows the results of studies on the influence of heavy metal ions (manganese, nickel, lead) on the viability and metabolic enzyme activity of marbled crayfish Procambarus virginalis (Lyko, 2017) (Decapoda). Due to the fact that marbled crayfish got into the reservoirs of the Dnipropetrovsk region in 2015, it was necessary to study the possibilities of its adaptation to environmental factors of reservoirs for further prediction of its distribution or even acclimatization under conditions of toxicological contamination of the ponds of the steppe Prydniprovya. In the experiment with marbled crayfish, chronic effects of various concentrations of heavy metal ions on the physiological state and enzyme activity were investigated. The obtained results showed that among the investigated heavy metals nickel ions influenced the weight indexes and mortality of crustaceans the most negatively. According to the results of the research, significant changes were noted in the individual biochemical parameters of marbled crayfish under the influence of manganese, lead and nickel ions. The most significant changes in the activity of lactate dehydrogenase were detected in muscle tissues affected by manganese and nickel ions. A significant decrease in the activity of succinate dehydrogenase in muscle of marbled crayfish was determined after the action of heavy metal ions. Investigation of changes in the activity of alkaline phosphatase under the influence of the ions of manganese, lead and nickel has its own characteristics, which indicates certain violations in the tissues of cell membranes. Changes in the activity of enzymes were also reflected in the overall protein content. In conclusion, changes in these parameters may indicate a rapid biochemical response of crustaceans to the toxic effects of heavy metals.
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