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1

Simultaneous HPLC determination of pomolic, ursolic and euscaphic/tormentic acids in roots and rhizomes of various Potentilla species

100%

Jóźwiak A. , Jóźwiak G. , Waksmundzka-Hajnos M.

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tom Vol. 26, no. 1

97--110

EN

A reversed-phase high-performance liquid chromatography diode array detector (HPLC-DAD) method was developed for the separation of triterpene acids and their determination in crude extracts of Potentilla species. Symmetry octadecylsilyl silica (ODS) column with acetonitrile and water with phosphoric acid (pH = 2.5) as the mobile phase was used in the described HPLC system. The column temperature was at 20 °C, the flow rate was 0.8 mL/min. The detection wavelength was 210 nm. For quantitative purposes, the linear dependencies of analyte concentration and peak surface area were obtained with regression coefficient values 0.9983 < r2 < 0.9995. For euscaphic acid, calibration was performed in the range of 0.07–0.525; 0.7–2.1 mg/mL, for pomolic acid in the range of 0.019–0.171; 0.209–0.665 mg/mL, for ursolic acid in the range of 0.2–1.375–3.3 mg/mL. The observed recovery of triterpene acids was from 95.3% to 103.1%. The observed values of RSD were well within the generally acceptable limit of no more than 5%. This method was successfully applied to quantify pomolic acid (PA), ursolic acid (UA) and the sum of euscaphic (EA) and tormentic acids (TA) (expressed as euscaphic acid) in roots and rhizomes of 15 various Potentilla species and 2 subspecies (i.e., P. atrosanguinea, P. recta).

2

The occurrence of unique, long-chain polyprenols in the leaves of Potentilla species

84%

Swiezewska E. , Chojnacki T.

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nr 2

3

Leaf indumentum types in Potentilla [Rosaceae] and related genera in Iran

67%

Faghir M. B. , Attar F. , Farazmand A. , Ertter B. , Eriksen B.

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tom 79

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nr 2

139-145

EN

Indumentum types of the leaves in 31 species of Potentilla L. (Rosaceae) and four related genera, especially Tylosperma Botsch., Schistophyllidium (Juz. ex Fed.) Ikonn., Drymocallis Fourr. ex Rydb., and Sibbaldia L. from Iran were investigated. Indumentum ultrastructure was studied by scanning electron microscopy (SEM). SEM observation revealed three type classes based on leaf indumentum: 1) straight (appressed-erect); 2) straight-erect and crispate, and 3) crispate-floccose. The straight hair character (I type class) is widely distributed among all genera sampled and six sections of Potentilla. In contrast the crispate-floccose indumentum (III type class) is confined to all examined species of sections Speciosae and Pensylvanicae. While some sections especially Rectae (straight and straight-crispate hairs) and Terminales (straight-crispate and floccose-crispate) posses two indumentum type classes. The present survey shows that indumentum types are of systematic importance and may form good key characters for identification purposes.

4

Response of three ornamental plant species to inoculation with arbuscular mycorrhizal fungi depending on compost addition to peat substrate and the rate of controlled release fertilizer

67%

Matysiak B. , Falkowski G.

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nr 2

321-333

EN

controlled release fertilizer (CRF) on mycorrhizal colonization, nutrient acquisition, and growth of Physocarpus opulifolius, Spiraea japónica and Potentilla fruticosa was investigated in a container experiment. The AMF treatments consisted of a commercial inoculum Endorize-Mix and non-inoculated (non- AMF) control. Compost was mixed with peat at different rates (0, 10 and 20% v/v). The CRF was applied at 100 and 50% of the manufacturer's recommended rate. Shoot fresh weights of all three species were affected by mycorrhizas the first year they were grown in containers. Differences between AMF and non-AMF plants were less significant in the second year. In the first year, species growth response to AMF inoculation was positive for P. fruticosa, which was not dependent on CRF rate and compost addition to substrate. Species growth response to AMF inoculation ranged from neutral to negative in the first year for P. opulifolius and S. japonica depending on the CRF rate and compost addition to substrate. Mycorrhiza development was generally supported by increasing the rate of compost in peat substrate and suppressed under the recommended rate of CRF. AMF inoculation affected P content in plant tissue of all species, with a higher P content in AMF than in non-AMF plants, regardless of the rate of CRF. However, the increased level of mycorrhizal colonization caused by AMF inoculum and higher phosphorus content in the leaves of all tested species did not correspond with higher biomass of plants. The greater participation of compost was in peat substrate, the more phosphorus and potassium contained all tested species. Shoot nitrogen content was not affected by AMF nor compost addition to peat substrate.

PL

Badano wpływ szczepionki mikoryzowej zawierającej grzyby arbuskularne AMF, dodatku kompostu do podłoża torfowego oraz dawki nawozu o kontrolowanym działaniu (CRF) na zasiedlanie korzeni przez grzyby mikoryzowe, pobieranie składników mineralnych oraz wzrost roślin Physocarpus opulifolius, Potentilla fruticosa i Spiraea japonica uprawianych w pojemnikach. W badaniach zastosowano komercyjną szczepionkę mikoryzową Endorize-Mix. Rośliny kontrolne nie były mikoryzowane. Kompost zmieszano z torfem w dawkach 0, 10 i 20% (v/v). Nawóz CRF stosowano w dawce zalecanej przez producenta (100%) i obniżonej o połowę (50%). Mikoryza- cja wpfywafe istotnie na wzrost roślin w pierwszym roku uprawy w pojemnikach, ale reakcja poszczególnych gatunków była odmienna. Inokulacja roślin szczepionką mikoryzową stymulowała wzrost P. fruticosa niezależnie od dawki CRF i kompostu w pod łożu torfowym. Z kolei w przypadku P. opulifolius i S. japonica oddziafywanie szczepionki mikoryzowej uzależnione było zarówno od dawki CRF, jak i udziału kompostu w podłożu torfowym i reakcja ta miała charakter neutralny lub negatywny. W drugim roku różnice między roślinami mikoryzowanymi i niemikoryzowanymi były mniej istotne. Zasiedlaniu korzeni przez grzyby mikoryzowe sprzyjał dodatek kompostu do podłoża torfowego i proces ten był hamowany przy zastosowaniu zalecanej dawki CRF. Wszystkie badane gatunki ro ślin inokulowane szczepionką miko- ryzową zawierały więcej fosforu w liściach niż niemikoryzowane niezależnie od dawki CRF. Wraz ze wzrostem dawki kompostu zwiększała się zawarto ść fosforu i potasu w roślinach. Zawarto ść N w liściach nie była uzależniona ani od zastosowania szczepionki mikoryzowej, ani od dodatku kompostu do substratu torfowego.