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Simultaneous determination of six alkaloids in Phellodendron amurense by high-performance liquid chromatography

100%

Li C. D. , Liu Y. , Shi Y. P.

Acta Chromatographica

2013

tom Vol. 25, no. 2

275--285

A high-performance liquid chromatography (HPLC) method has been developed for simultaneous determination of six alkaloids, i.e., (−)-(R)-platydesmin, noroxyhydrastinine, berberine, skimmianine, canthin-6-one, and pteleine in the herbal medicine of Phellodendron amurense Rupr. The optimal condition for extraction and separation was achieved with a linear mobile phase gradient prepared from 0.1% phosphoric acid and acetonitrile. The LODs and LOQs for the analytes ranged from 0.06 to 0.22 μg mL-1 and from 0.25 to 0.80 μg mL-1, respectively. The optimized method was applied to the determination of alkaloids in P. amurense Rupr. and was found to be efficient. This method can provide a scientific and technical platform to the manufacturers for setting up a quality control standard as well as to the public for quality and safety assurance of the proprietary traditional Chinese medicines.

The effect of a sawing angle on wood texture

72%

Mayevskyy V. , Maksymiv V. , Sopushynskyy I. , Teischinger A.

Folia Forestalia Polonica. Series B - Drzewnictwo

2008

tom 39

Malo znane - warte uwagi

50%

Gorka W.

nr 6