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1

Wystepowanie bakterii beztlenowych w popluczynach pecherzykowo-oskrzelikowych u chorych po operacjach torakochirurgicznych

100%

Zajac-Lenczewska I. , Kedzia A. , Skokowski J. , Sternau A.

Medycyna Doświadczalna i Mikrobiologia

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2003

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tom 55

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nr 2

173-180

PL

Wykonano bronchofiberoskopie z pobraniem popłuczyn pęcherzykowo- oskrzelikowych (BAL) u 30 chorych po operacjach torakochirurgicznych w celu oceny beztlenowej flory bakteryjnej dolnych dróg oddechowych. Łącznie wyizolowano 100 szczepów bakterii beztlenowych. Wśród Gram-ujemnych bakterii dominowały Prevotella oraz Bacteroides. Charakteryzowały się one nąjwiększą wrażliwością na imipenem, piperacylinę z kwasem klawulanowym i metronidazol. Spośród Gram-dodatnich bakterii najczęściej były izolowane: ziarniaki z rodzaju Peptostreptococcus wrażliwe na wszystkie badane środki przeciwbakteryjne oraz pałeczki z rodzaju Actinomyces - wrażliwe na wszystkie badane antybiotyki, z wyjątkiem metronidazolu.

EN

Purpose of this study was to find out what kind of anaerobic bacteria were in lower respiratory tract and how often they were present there considering patients after thoracic surgery. Also, what is susceptibility of bacteria to antibiotics. Research covered 30 patients after operation. Material for research was bronchoalveolar lavage (BAL) taken during bronchoscopy. Collected sample was cultivated in anaerobic and aerobic conditions. Anaerobic bacteria were found in 28 samples (93%). Totally there were 100 anaerobic bacteria strains. The most common Gram-negative rods were from genus Prevotella (24 strains, 24%) and Bacteroid.es (15 strains, 15%). Gram-negative bacteria except Bacteroides characterised biggest susceptibility to imipenem, piperacillin/tazobactam, amoxicillin/clavulanate, ampicillin/sulbactam, piperacillin, clindamycin and metronidazol. Bacteroides were susceptible to imipenem, piperacillin/tazobactam and metronidazol. Among Gram-positive anaerobic bacteria mostly were isolated from cocci Peptostreptococcus (18 strains, 18%) and were susceptible to all antibiotics. Gram-positive rods were in most cases represented by Actinomyces (12 strains 12%) and were highly susceptible to all antibacterial means except metronidazol (100% is resistant).

2

Identyfikacja i lekowrazliwosc bezwzglednie beztlenowych bakterii wyizolowanych od hospitalizowanych pacjentow

80%

Kot K. , Rokosz A. , Sawicka-Grzelak A. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

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2002

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tom 54

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nr 3

233-241

PL

Od pacjentów hospitalizowanych w 2001 roku wyhodowano 225 szczepów bezwzględnych beztlenowców, w tym 54 szczepy bakterii Gram-ujemnych i 171 szczepów bakterii Gram-dodatnich. Wśród Gram-ujemnych bakterii dominowały szczepy Bacteroides fragilis, a wśród Gram-dodatnich szczepy Peptostreptococcus sp. Stwierdzono, że lekami najbardziej aktywnymi wobec klinicznych szczepów Gram-ujemnych bakterii beztlenowych były: metronidazol, piperacylina z tazobaktamem, tikarcylina z kwasem klawulanowym i imipenem, a wobec szczepów Gram-dodatnich bakterii beztlenowych: piperacylina z tazobaktamem, amoksycyiina z kwasem klawulanowym, tikarcylina z kwasem klawulanowym i imipenem.

EN

The aim of this study was to identify anaerobic strains isolated in 2001 from clinical specimens obtained from patients of Warsaw hospital and to evaluate a susceptibility of these strains to antimicrobial agents. In 2001 two hundred and twenty five clinical strains of obligate anaerobes were cultured, which were identified in the automatic ATB system (bioMérieux, France) using biochemical tests API 20 A. Drug-susceptibility of strains was determined also in ATB system with the use of ATB ANA strips. C. difficile strains were isolated on selective CCCA medium. Toxins A/B of C. difficile directly in stool specimens were detected by means of ELISA test (TechLab, USA). Fifty four strains of Gram-negative anaerobes (B. fragilis strains dominated) and 171 of Gram-positive anaerobes (the greatest number of strains belonged to genus Peptostrptococcus) were cultured from clinical specimens. In the cases of antibiotic-associated diarrhea 28 C. difficile strains were isolated and C. difficile toxins A/B were detected in 39 stool samples. The most active in vitro antimicrobials against Gram-negative anaerobes were metronidazole, imipenem, ticarcillin combined with clavulanic acid and piperacillin with tazobactam. Gram-positive, clinical strains of anaerobes were the most susceptible in vitro to ß-lactam antibiotics combined with ß-lactamase inhibitors (amoxicillin/clavulanate, piperacillin/tazobactam, ticarcillin/clavulanate) and imipenem.

3

Flora bakteryjna w zapaleniu gardla i migdalkow

80%

Radosz-Komoniewska H. , Rogala-Zawada D. , Zientara M. , Rudy M. , Nowakowska M.

Medycyna Doświadczalna i Mikrobiologia

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1998

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tom 50

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nr 1-2

63-68

PL

W pracy przedstawiono częstość izolacji poszczególnych gatunków bakterii z wymazów z gardła u 157 chorych z rozpoznaniem zapalenia gardła oraz z próbek ropnego wysięku z krypt migdałków u 10 chorych leczonych z powodu przewlekłego ropnego zapalenia migdałków podniebiennych. Paciorkowce ß-hemolizujące wyosobniono od 30% badanych. Najczęściej izolowano Streprtococcus pyogenes (12%) i paciorkowce grupy C (10,7%). Większość wyosobnionych bakterii należała do flory potencjalnie chorobotwórczej (70%). Bakterie beztlenowe wyosobnione z ropnego wysięku z krypt migdałków zaklasyfikowano do rodzaju Prevotella, Fusobacterium, Peptostreptococcus i Gemella.

EN

The aim of the study was a microbiological analysis of pharyngeal swabs obtained from 158 patients with the diagnosis of pharyngitis and purulent exudates from the tonsillar crypts of 10 patients treated for chronic purulent tonsillitis. Beta haemolytic streptocoocci groups A, B, C and G were isolated from 30% of the patients. The most frequently isolated were Streptococcus pyogenes - 12% of patients and Streptococcus group C - 10,7%. Other streptococci were isolated less frequently: Streptococcus group B - 44%, group G - 2,5%. The majority of isolated bacteria belonged to potential pathogenic flora (70% patients). Staphylococcus aureus (37%) and Haemophilus spp. (36%) were isolated most frequently. Other bacteria were isolated in the following sequence: Moraxella catarrhalis - 22%, Streptococcus pneumoniae - 17% and Gram- negative rods from the Enterobacteriaceae family - 6%. One case of Plaut-Vincent tonsillitis was diagnosed. Aerobic and anaerobic bacteria were isolated from purulent exudates from the tonsillar crypts of 10 patients treated for chronic purulent tonsillitis. The isolated anaerobic bacteria belonged to genus of Prevotella, Fusobacterium, Peptostreptococcus and Gemella.