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Zastosowanie AAV jako wektorow w terapii genowej

100%

Kamieniarz K. , Gozdzicka-Jozefiak A.

Biotechnologia

2005

nr 1

79-94

Construction and immunogenic properties of DNA vaccine against canine parvovirus infection

100%

Mizak B. , Rzezutka A. , Krol J. , Kazula A.

Bulletin of the Veterinary Institute in Puławy

2001

tom 45

nr 2

The presented studies were concentrated on construction of the recombinant mammalian expression vector - pSecTag2B, carrying canine parvovirus VP2 gene and its N-terminal fragment. Evaluation of safety of experimental vaccines was done on laboratory animals and immunogenic properties were tested on dogs. CPV specific antibodies were not detected by HI test in serum samples collected from dogs vaccinated with recombinant plasmid pSecTag2B-CPV582. The presence of CPV specific antibodies (80-320 by HI) was confirmed 10 days after vaccination in all dogs vaccinated with pSecTag2B-CPV1774. The highest titre was obtained in sera collected from dogs vaccinated with 200 μg and 300 μg of DNA. Fourteen days after revaccination, the level of antibodies increased up to 160 in dogs vaccinated with 100 μg of DNA and up to 640 in puppies vaccinated with 200 μg and 300 μg of DNA. The antibodies were detected for at least 16 weeks. Results of the studies demonstrated the immunogenic properties of elaborated DNA vaccine.

Molecular characterisation and genetic diversity of canine parvovirus type 2 prevalent in Central China

84%

Hu W. , Xu X. , Liu Q. , Ji J. , Kan Y. , Yao L. , Bi Y. , Xie Q.

Journal of Veterinary Research

2020

tom 64

nr 3

Touchdown PCR for the detection of waterfowl parvoviruses

67%

Wozniakowski G. , Kozdrun W. , Samorek-Salamonowicz E. , Krol K.

nr 1

3-7

Sixteen field strains of goose parvovirus (GPV) and DNA extracted from standard strain of Muscovy duck parvovirus (MDPV) were used. PCR was used for the amplification of the VP3 structural protein encoding region. In the case of all GPV strains and one MDPV strain the presence of specific product about 1,604 bp long was observed. The essential conditions, which had the influence on the specificity, sensitivity, and efficiency of the amplification reaction, were optimised. In order to eliminate unspecific interactions between template and primers touchdown PCR was applied. Additionally, for specificity and efficiency improvement, betaine (N, N, N - trimethylglycine) was added. The conducted optimisation steps of PCR allowed for the identification of the both parvoviruses.