Wyniki wyszukiwania - Biblioteka Nauki

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Purification and properties of chitin deacetylase from Absidia orchidis

100%

Szewczyk K. W. , Orzeszak M.

Biotechnologia

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2002

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nr 2

48-59

EN

Methods of purification of chitin deacetylase are discussed. A two step method of purification of chitin deacetylase from mycelial extracts of the fungus Absidia orchidis by chromatography is presented. The crude enzyme extract was purified by a gel chromatography and then by ion exchange chromatography. Specific activity of purified enzyme was 12.3 U/mg and final purification degree was 147. The apparent molecular mass of the enzyme was 75 kDa. When O ? hydroxyethylated chitin (glycol chitin) was used as a substrate, the optimum pH for enzyme activity was 5,5 and the optimum temperature was 50?C.

2

Purification and some characteristics of Penicillium citrinum lipase

80%

Maliszewska I. H.

Biotechnologia

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1997

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nr 3

109-120

EN

An extracellular lipase (glycerol ester hydrolases E.C. 3.1.1.3.) was isolated from a culture filtrate of Penicillium citrinum. The purification procedure included ammonium sulfate precipitation, ultrafiltration and chromatography on Octyl-Sepharose CL-4B. The enzyme was 400-fold purified with 9.66% yield. The molecular weight has been estimated by polyacrylamide gel electrophoresis under denaturing conditions at 26000. On the other hand, lipase forms active dimers and tetramers aggregates as observed after native PAGE. Lipase from Penicillium citrinum showed a preference for triacylglycerols. It is non-specific and hydrolyzes each of the three ester bonds of triacylglycerols. The enzyme showed a maximum activity at pH 7.2 at 30 oC and was stable in the range of pH 6.0-7.5 and the temperature of 10oC - 40oC.

3

Założenia i perspektywy fenomenologicznej antropologii filozoficznej) Source: Zeszyty Naukowe Uniwersytetu Ekonomicznego w Krakowie

80%

Węgrzecki A.

Zeszyty Naukowe Uniwersytetu Ekonomicznego w Krakowie

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2012

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nr 882

47-56

EN

In the history of philosophy, the belief that there is a need to systematically build a philosophical conception of man grew gradually. Max Scheler believed that there is no task more urgent than building philosophical anthropology. It can be assumed that the idea to give it a phenomenological character was close to his heart. However, he was not consistent in applying the phenomenological procedure, since he incorporated scientific conclusions and also referred to metaphysical assumptions. Phenomenological philosophical anthropology would have to observe the principle of presuppositionlessness and to draw its content from phenomenological experience. It should also not disregard facts provided by exact sciences but subject them to a “cleansing” under the control of the said experience.

4

VÝZNAM OBŘADU ŽEHNÁNÍ MATKY PO PORODU

70%

Vlčková M.

Studia theologica

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2013

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tom 15

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nr 1

36–46

EN

This work deals with the significance of the ceremony of blessing the mother after birth. It focuses primarily on the liturgical ceremony of blessing the mother, its development in history and its significance in liturgical-theological and socio-cultural terms. The concept of blessing the mother is used here as a term including both the ceremony known as introduction to confinement – introductio mulieris post partum, (or benedictio mulieris post partum), the history of which reaches back to the liturgy of the Catholic church in the 11th century and ends with the Second Vatican Council, and the forms of blessing the mother revived as part of the liturgical reform of the Second Vatican Council; the blessing prayer at the end of the baptismal ceremonies for the baptism of the child and Ordo benedictionis mulieris post partum in accordance with the new Benedictional (1984). The work is concluded by a reflection on the present and future practice of blessing the mother.

5

Regeneracja oleju z orzecha włoskiego adsorbentami pozyskanymi z jego łupin

70%

Buczek B. , Kozera P.

Zeszyty Naukowe Uniwersytetu Ekonomicznego w Krakowie

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2012

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nr 899

5-14

EN

The paper presents the treatment by physical and chemical activation of oxidised oil from walnuts on carbon adsorbents obtained from walnut shells. Estimated sorption properties of activated carbons were shown on the methylene and iodine adsorption number. In order to assess the oil regeneration process, the following quality indicators were investigated: the peroxide number, the anisidine value, the total acid number, the iodine value and the Totox value, colour substances and the fatty acid profile. Following the regeneration process, a reduction in primary and secondary oil oxidation products was observed, leaving the fatty acid profile practically unchanged. This indicates that oil has a high affinity and selectivity toward the adsorption of the products of its own decomposition. The thesis is shown to be valid. That is, the period of use following treatment of oxidised oil on adsorbents obtained from walnut shells can be extended.

6

„Za čistší město“: Problémové lokality a jejich obyvatelé z pohledu místní politiky a správy

51%

Vacková B. , Galčanová L. , Hofírek O.

Sociologický časopis. Czech Sociological Review

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2011

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tom 47

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nr 4

633-656

EN

Drawing on the results of a qualitative study conducted in twelve Czech cities, the authors discuss how ‘problematic localities’ are represented in the narratives of local politicians and public officials. They analyse the ways in which these localities are categorised and how these categorisations are used to legitimise the specific treatment of these places and their inhabitants. City governance and administration are considered to be a part of a modern tradition of urban planning and city management, which is analysed in the first part of the study. The second, empirical part shows how ‘common sense’, ethnicised attributes are activated and applied to the localities and their inhabitants in the narratives of politicians and public officials. These attributes are associated with a notion of impurity, which leads to the need for surveillance, discipline, or purifi cation. From their analysis of these narrative practices the authors suggest that the borders of entitlement and the borders of responsibility are constructed. The borders of entitlement define who deserves the care provided by a state or a city; the borders of responsibility then delimit the symbolic space in which the state or city is perceived by its representatives to be responsible for the situation of its inhabitants and citizens. A crucial role is played in the process of border formation by (1) the application of ethnicised categories and inconsistent definitions of the objects of municipal and state care and by (2) the forms of ownership that apply to the housing stock in which these objects, that is, people, live.