Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

3 Folia Biologica

Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: PARAMECIUM TETRAURELIA

Sortuj według:

Ogranicz wyniki do:

Effect of cytoplasm containing immaturin - a protein controlling mating immaturity - on the occurence of autogamy in Paramecium tetraurelia

100%

Kosciuszko H. , Prajer M.

nr 1-2

1-4

Sexual immaturity in the Paramecium aurelia species complex is genetically controlled, its duratin being expressed by the number of cell divisions between successive conjugations or autogamies.During post-conjugational immaturity mating eractivity is regulated by a cytoplasmic protein - immaturin.The present study revealed that cytoplasm containing immaturin of Paramecium primaurellia injected into preautogampus cells of Paramecium tetraurelia does not inhibit the latter in entering autogamy.This results suggests that immaturin is inefective in the inhibition of autogamy.

Effect of wild-type macronucleoplasm transplantation into trichocyst mutant cells of Paramecium tetraurelia

100%

Prajer M. , Kosciuszko H.

Folia Biologica

2001

tom 49

nr 3-4

125-128

Approximately 1/2 of the macronucleoplasm of wild-type cells of Paramecium tetraurelia was transplanted into d4-84 mutant recipient homozygous for mutations nd3a (non-discharging trichocysts) and ts401 (temperature sensitive). After injection, 30% of surviving cells shifted from mutant to wild-type phenotype. Among the remaining cell lines 29% were unable to eject any trichocysts, and 41% discharged less than 10 of them per cell, when tested with picric acid. Observations were made through two successive vegetative fissions. These results showed that 30% of d4-84 cells contained foreign gene responsible for trichocyst discharge and produced cell lines of the wild-type phenotype, in which the expression as well as replication of this gene appeared normal.

Anomalies of autogamy in Paramecium tetraurelia temperature sensitive mutant induced by heat treatment

88%

Prajer M. , Iftode F.

nr 1-2

13-27

The run of autogamy in temperature sensitive mutant ts401, carrying also the mutation of trichocysts nd3a, at permissive (26oC) and non-permissive temperature (35oC) was studied and compared with this process in wild d4-2 stock of Paramecium tetraurelia. The effect of heat treatment on programmed nuclear and cortical events was investigated using cytological silver impregnation Fernandez-Galiano method and immunofluorescence technique with the application of two anti-a-tubulin antibodies. The appearance of cells with some large macronuclear fragments and an excess number of micronuclei suggests that nuclear differentiation is inhibited at restrictive temperature and that macronuclear regeneration takes place in this thermosensitive mutant. In such cells impaired oral reorganization was observed. After slight refeeding at the late autogamy, cells stopped at first postautogamous division were induced by heat treatment. The most striking feature of these abnormal divisions was the lack of a basal body duplication wave, which suggests that production of g-tubulin, structural protein being the essential component of the microtubule organizing centres, is disturbed at high temperature.