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The role of nitric oxide and NADPH oxidase in short term signalling events activated in soybean seedlings by cadmium

100%

Chmielowska-Bak J. , Deckert J.

nr 2

Ethylene mediates in the early oxidative stress induced by mercury

84%

Montero-Palmero M. , Martin-Barranco A. , Alonso J. , Escobar C. , Hernandez L.

nr 2

NADPH oxidase mediated ROS control susceptibility of Arabidopsis plants to a parasite

84%

Siddique S. , Matera C. , Hassan S. , Sobczak M. , Rozanska E. , Gutbrod P. , Grundler F.

nr 2

Direct, calcium-independent, regulation of NADPH oxidase during plant innate immunity

84%

Kodota Y. , Sklenar J. , Derbyshire P. , Stransfeli L. , Asai S. , Ntoukakis V. , Shirasu K. , Jones A. , Zipfel C.

nr 2

Signalling via reactive oxygen species – why and how?

67%

Bartosz G.

nr 2

Trans fatty acids induce a proinflammatory response in endothelial cells through ROS-dependent nuclear factor-kappaB activation

67%

Bryk D. , Zapolska-Downar D. , Malecki M. , Hajdukiewicz K. , Sitkiewicz D.

nr 2

It has been shown that increased intake of trans fatty acids (TFAs) is associated with a higher risk of cardiovascular disease. In this study, we have investigated the effects of linoelaidic (LA) and elaidic (EA) acids on the proinflammatory response in endothelial cells, a key step in vascular disease. Human aortic endothelial cells (HAECs) were treated with different concentrations (100 µmol/l in most experiments) of LA or EA for different periods of time. The surface protein and mRNA expression of ICAM-1 and VCAM-1 were determined by flow cytometry and real time RT-PCR, respectively. Adhesion of leukocytes to TFA-treated HAECs was evaluated by an adhesion assay. Activation of nuclear factor-B (NF-B) was evaluated by measuring NF-B p65 phosphorylation using flow cytometry. ROS production was determined by the reduction of fluorescent 2',7'-dichlorofluorescein diacetate (DCFH-DA). LA treatment significantly increased protein and mRNA levels of ICAM-1 and VCAM-1, leukocyte adhesion to HAECs, phosphorylation of NF-B and ROS generation. Similar effects were achieved for cells incubated with EA. Experiments with HAECs pretreated with pyrrolidine dithiocarbamate, an inhibitor of NF-B, revealed that both LA and EA-mediated induction of ICAM-1 and VCAM-1 is mainly regulated by NF-B. The ROS production induced by both of the studied acids was inhibited in the presence of diphenyleneiodonium (DPI), a NADPH oxidase inhibitor, suggesting ROS production through the activation of NADPH oxidase. Furthermore, LA or EA-induced ICAM-1 and VCAM-1 expression, activation of NF-B and adhesion of leukocytes to HAECs were abolished in the presence of DPI. Conclusion: TFAs present in our diet have a direct proinflammatory effect, which promotes leukocyte adhesion to the endothelium through ROS-dependent NF-B activation.

Immunoreceptor signaling in innate and adaptive immune system cells

59%

Swat W.

tom 59

nr 5