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Zastosowanie polianiliny w czujnikach amperometrycznych do oznaczania NADH

100%

Kossakowska A. , Pijanowska D. G. , Kruk J. , Torbicz W.

2010

tom Vol. 51, nr 6

140-141

W artykule przedstawiono modyfikację amperometrycznych czujników do oznaczania NADH. Czujniki z elektrodami platynowymi modyfikowano za pomocą polianiliny (PAn), dobierając parametry elektropolimeryzacji aniliny, w tym: potencjał i czas polimeryzacji oraz stężenie monomeru. Największą czułość na NADH uzyskano w przypadku osadzania PAn z roztworu 0,3 M An metodą chronoamperometryczną przy potencjale 0,75 V w czasie 3 min. Zastosowanie polianiliny jako materiału elektrody, poza zwiększeniem czułości czujnika, powoduje obniżenie potencjału utleniania NADH.

In this paper, modifications of amperometric sensors for the determination of NADH are described. The sensors with platinum electrodes were modified with polyaniline (PAn) deposited in different aniline electropolymerization processes tuned by potential and time of electropolymerization as well as monomer concentration. Based on calculated sensitivity to NADH, the best conditions of electropolymerization were chosen: 0.3 M An, 0.75 V and 3 min. Application of polyaniline as an electrode material causes: reduction of oxidation potential of NADH by 400 mV, higher current values and increase in sensitivity of the sensor for NADH.

Dehydrogenation studies of dihydronicotinamide adenine dinucleotide (NADH) with methylene blue in the presence of the copper hexcyanoferrate(II) complex and light

99%

Tewari B.

Open Chemistry

2005

tom 3

nr 3

441-451

The effects of copper ferrocyanide and light on the dehydrogenation rate of NADH by methylene blue is studied. The results suggest that the dehydrogenation rate of NADH with methylene blue is enhanced by copper ferrocyanide. Light also affects the reaction rate.

Detection of NADH and ethanol at a graphite electrode modified with titania sol-gel/Meldola’s Blue/MWCNT/Nafion nanocomposite film

99%

Kochana J. , Adamski J.

Open Chemistry

2012

tom 10

nr 1

224-231

For electrocatalytic determination of NADH, a graphite electrode modified with titania sol-gel/Meldola’s Blue/MWCNT/Nafion nanocomposite was proposed. The composition of the matrix film was optimised in terms of the content of carbon nanotubes and Nafion. Incorporation of a redox mediator, Meldola’s Blue, into the nanocomposite film enabled electrocatalytic determination of NADH at a low potential, −50 mV. For determination of ethanol, alcohol dehydrogenase (ADH) was immobilized into the matrix layer. Experimental conditions affecting the biosensor response were examined, including enzyme loading, temperature of measurement and pH of background electrolyte. Assessments of the analytical characteristics of the biosensor were performed with respect to sensitivity, limit of detection, operational stability, repeatability and reproducibility. The proposed biosensor showed electrocatalytic activity toward oxidation of ethanol with sensitivity of 2.24 µA L mmol−1, linear range from 0.05 to 1.1 mmol L−1, and limit of detection of 25 µmol L−1. The apparent Michaelis-Menten constant was 1.24 mmol L−1, indicating a high biological affinity of ADH/titania sol-gel/Meldola’s Blue/MWCNT/Nafion electrode for ethanol. The developed biosensor was tested in determinations of ethanol content in alcoholic beverages.