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EN
Effects of heavy seed crop (mainly oak mast) in year 2003 and 2004 (no seed crops) on small mammal communities in three isolated stands of broad leaved lowland forests (area 60-280 ha) with different management were studied in southern Moravia in three sites: (1) close-to-nature not managed floodplain forest - Ulmeto-Fraxinetum carpineum (FF), (2) managed forest - Carpineto-Quercetum acerosum (MF), (3) pheasantry - Ulmeto-Fraxinetum carpineum, Saliceto-Alnetum and Carpineto-Quercetum acerosum, with a considerable number of biotopes including open areas (PH). We presumed the influence of seed production on mammal species, especially granivorous rodents. In the most numerous species (Apodemus flavicollis (Mel.), A. sylvaticus (L.) and Myodes glareolus (Pallas)) the demographic parameters (abundance, sex ratio), body mass, and body length were compared between popu-lations in 2003 and 2004 in each stand. Animals were trapped in snap mousetraps laid out in lines. In 2003 reproduction of both Apodemus sp. was extended to November in contrast to 2004, when it ceased already in the end of summer. Individual body mass of animals were significantly higher in 2004 vs. 2003 in all three species (A. flavicollis, P = 0.01; A. sylvaticus, P <0.01 and Myodes glareolus, P <0.05) but body length was higher only in case of A. sylvaticus (P <0.01). The forest stands under study in an intensively managed agroecosystem in southern Moravia play an important role as a refuge for small mammals.
EN
In promiscuous species in which females mate with more than one male during oestrus, males may increase their sperm expenditure or change their copulatory behaviour in response to the risk of sperm competition. I used an experimental approach to investigate the pattern of copulatory behaviour of the bank voleMyodes glareolus Schreber, 1780 depending on whether the female mated with one or two males. The work showed that the copulatory period of the bank vole lasted about 80 minutes and consisted of 4–5 ejaculatory series, with multiple intromissions preceding ejaculation. There were no significant changes in number of intromissions across the first four ejaculatory series, but I did find a relationship between number of intromissions and first ejaculation latency; also, ejaculation latencies grew shorter as the ejaculatory series proceeded. Litter size did not differ significantly between females that mated with one male and those mating with two, nor did the reproductive success of males that mated with the same female. Mating with an oestrus female appears to be advantageous for bank vole males even if they mate as the second one, and the risk of sperm competition did not trigger changes in male copulatory behaviour. The similar durations of the copulatory period and patterns of change of ejaculation latencies during copulations with one and two males point to the role of the female in temporal copulatory behaviour of the bank vole.
EN
Seasonal changes in lipid droplet size and lipid peroxidation in the brown adipose tissue (BAT) of wild bank voles were examined. In addition, a role of photoperiod in these changes was studied; bank voles were held from the birth under long photoperiod (LP) for 12 weeks, and then half of them was transferred to short photoperiod (SP) for 6 weeks and another one remained under LP. In the wild bank voles the absolute BAT weight was seasonally constant, while the significant differences in the lipid droplet size were observed. The smallest lipid droplets (mean, 11 μm2) were seen in winter; they increased by 30 % in spring and reached the highest size (24 μm2) in summer. Lipid peroxidation in the BAT did not differ significantly between the seasons, although high intraseason variation of this process was noted. The laboratory experiment revealed that the size of lipid droplets was determined by photoperiod; SP induced 13-fold decrease, and continuous exposure to LP brought about a further 2.5-fold increase in the size of lipid droplets. Conversely, a significant decrease in lipid peroxidation was seen in LP bank voles in comparison with the SP animals. The data indicate that short photoperiod is responsible for the small size of lipid droplets in the BAT of bank voles during winter, which may be a necessary requirement for high thermogenic capacity of the tissue. Photoperiod appears also to affect lipid peroxidation in the BAT of these animals.
EN
In recent years, the bank voleMyodes glareolus (Schreber, 1780) has emerged as a model system for parasitological, behavioural and ecological studies and seems ideally suited to address questions concerning the importance of MHC variation at individual and population levels. Here, we provide the first extensive survey of sequence variation in the MHC class II DRB genes in this species. Among 34 analysed voles we found 15 unique sequences, representing most likely two loci, at least one of them expressed. Despite very high overall sequence divergence, particularly in the Antigen Binding Sites (ABS), we detected signatures of positive selection that has been acting on DRB in the bank vole. Phylogenetic analysis demonstrated that the bank vole DRB alleles do not form a monophyletic group but are intermingled with other rodent alleles that is consistent with long-term persistence of ancient allelic lineages maintained through balancing selection. Our sequence data will forward the design of efficient genotyping methods, which will permit testing hypotheses pertaining to the ecological causes and consequences of MHC variation in the bank vole.
EN
The bank voleMyodes glareolus Schreber, 1780 is a widely distributed rodent in Europe, being numerically dominant in small mammal communities living in temperate woodlands. However, it becomes scarce in southern Europe (Mediterranean area) where it reaches the southernmost limit of its distribution range. We studied the habitat preferences of bank voles in 9 plots in a transitional area between Mediterranean and Eurosiberian regions within a Mediterranean mountain. During the study period we captured 1919 small mammals of 9 species, including 287 bank voles (14.95%). Mean density ranged from zero individuals per plot (1.1 ha) at the boreo-subalpine scrubland to 10.27 ± 2.84 (SE) at a Mediterranean river woodland. Statistical path analysis was used to investigate relationships between mean bank vole density and climate and vegetation structure measured within plots. The variables selected by the structural equation model were those related to forest structure, like tree cover and height, dead vegetation, moss, and rock cover. Habitat moisture was also important (microclimatic conditions). Mean climate conditions (and elevation) did not have any significant effect on mean bank vole density, and no significant association with understorey vegetation (eg shrub and herbaceous cover) was observed. Our results pointed out that bank voles were habitat specialists in our study area, being more abundant and frequent in moist woodlands, and rare or absent in shrublands and grasslands.
EN
Difficulties in investigating shrews in the wild in winter, especially in trapping them and keeping them alive during live-trapping studies, have been the main reason for serious deficiencies in our knowledge of their ecology. We developed a live-trapping protocol which allowed us to maximise capture rates and minimise mortality of shrews. We used wooden box traps with a nest-chamber, which we set in plywood ‘chimneys’ with removable roofs. Chimneys facilitated suitable positioning of traps and protected them from being blocked by snow. This resulted in a high trappability (up to 20.2 shrews and 8.2 voles per 1000 trap hours), a large proportion of recaptures (most shrews were recaptured, often repeatedly) and a very low mortality rate (<0.09 shrews and 0 rodents per 1000 trap hours) despite sub-zero temperatures and deep snow cover. This allowed us to pursue an intensive live-trapping study, using the CMR-method, of shrews wintering in the Narewka river valley (north-east Poland). Because of the high trappability and minimal mortality, the presented protocol can be recommended to study winter ecology and conservation biology of such fragile and strictly protected small mammals as shrews.
EN
Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to flooding. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of hantavirus RNA. Out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. Hantavirus prevalence was low in Apodemus agrarius (2.6%), the most numerous mammal species, whereas in the remaining 3 positive species (Microtus agrestis, Myodes glareolus, Sorex araneus) this was 12.5–100%. The presence of hantaviruses was detected only in the animals found on area ‘A’ exposed to flooding, and their prevalence was statistically greater compared to area ‘B’ not exposed to flooding (16.7% vs. 0%, p=0.0345). The overall positivity of the examined small mammals population from the areas ‘A’ and ‘B’ was 8.3%. The sequence analysis of the samples positive for hantavirus proved that the amplified products showed 77–86% homology with the L segment sequence of hantavirus Fusong-Mf-731 isolated from Microtus fortis in China. The presented study is the first to demonstrate the occurrence of hantavirus infection in small mammals from eastern Poland, and the first to demonstrate the significant relationship between flooding and the prevalence of hantaviruses in small mammals.
EN
An examination of 16 bank voles from Poland (Pomerania) revealed the presence of two species of the family Demodecidae (Acari, Prostigmata), specific to the host. Demodex buccalis Bukva, Vitovec et Vlcek, 1985 was noted only in one bank vole, where 18 specimens were found: the prevalence of infestation being 6.3%. D. glareoli Hirst, 1919 was observed in 75% of the examined bank voles, in which were on average 5.1 specimens. Additionally, mites of the both species exhibited topical specificity – representatives of D. buccalis were found in the tissues of the tongue and oral cavity of the host, while D. glareoli, being a species associated with hair follicles, was noted in skin specimens from different body areas, particularly the head area. Infestations with demodecids were not accompanied by disease symptoms. D. buccalis and D. glareoli are a new species for the fauna of Poland.
EN
Effects of heavy seed crop (mainly oak mast) in year 2003 and 2004 (no seed crops) on small mammal communities in three isolated stands of broad leaved lowland forests (area 60–280 ha) with different management were studied in southern Moravia in three sites: (1) close-to-nature not managed floodplain forest – Ulmeto–Fraxinetum carpineum (FF), (2) managed forest – Carpineto-Quercetum acerosum (MF), (3) pheasantry – Ulmeto-Fraxinetum carpineum, Saliceto- Alnetum and Carpineto-Quercetum acerosum, with a considerable number of biotopes including open areas (PH). We presumed the influence of seed production on mammal species, especially granivorous rodents. In the most numerous species (Apodemus flavicollis (Mel.), A. sylvaticus (L.) and Myodes glareolus (Pallas)) the demographic parameters (abundance, sex ratio), body mass, and body length were compared between populations in 2003 and 2004 in each stand. Animals were trapped in snap mousetraps laid out in lines. In 2003 reproduction of both Apodemus sp. Was extended to November in contrast to 2004, when it ceased already in the end of summer. Individual body mass of animals were significantly higher in 2004 vs. 2003 in all three species (A. flavicollis, P = 0.01; A. sylvaticus, P <0.01 and Myodes glareolus, P <0.05) but body length was higher only in case of A. sylvaticus (P <0.01). The forest stands under study in an intensively managed agroecosystem in southern Moravia play an important role as a refuge for small mammals.
EN
Morphological analysis revealed destructive changes in the testicular tissue of bank volesMyodes glareolus Schreber, 1780 during different phases of a population fluctuation. The most pronounced changes were observed at the peak phase, when karyolysis of the Leydig cells and degeneration of spermatogenous cells were recorded in 90% of males. During the increase phase, depression of androgenous testicular function was observed in only 50% of males and atrophy of seminiferous tubules in 30% of males. During the low phase, the proportion of males with destructive changes in generative and endocrine portions of the testicles did not exceed 30%. Morphometrical analysis of spermatozoa demonstrated that the size of the head and nucleus were related to the phase of the population fluctuation. Lengths of the middle and main parts of the spermatozoon tail, as well as the size of the acrosome, were not related to phase of the population fluctuation.
EN
We studied inter-annual, spatial and sexual variation in the body mass of bank volesMyodes glareolus Schreber, 1780 and grey-sided volesMyodes rufocanus Sundevall, 1846 using live trappings from two grids on the southand north-facing slopes of a mountain valley in Southern Norway. Variation in spring density of the four populations was consistent with cyclic dynamics (n=7,s-values >0.5). Individuals caught on the south-facing slope were larger than those caught on the north-facing slope. Reproductively mature bank vole males were smaller than females, whereas reproductively mature grey-sided vole males were larger than females. Body mass was related to density in both species. In bank voles, we found a direct positive density dependence caused by a higher rate of survival at higher densities resulting from individual allocation of resources from reproduction to survival and growth. In grey-sided voles, we found a negative delayed density dependence resulting from grazing on preferred plants that determined the resource available for individual vole growth the following year.
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