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The composition of cell wall skeleton and outermost lipids of Mycobacterium vaccae is modified by ethambutol treatment

100%

Rumijowska-Galewicz A. , Korycka-Machala M. , Lisowska K. , Dziadek J.

Polish Journal of Microbiology

2008

tom 57

nr 2

Ethambutol (EMB) is a first line drug in tuberculosis treatment inhibiting the biosynthesis of arabinogalactan, which is a component of the mycobacterial cell wall. The growth of Mycobacterium vaccae cells in the presence of EMB increases cell wall permeability, which was monitored by β-sitosterol biotransformation. GC/MS and GLC/MS (gas chromatography/mass spectrometry) analysis revealed dramatic changes in the content of covalently bound mycolic acids and in molar ratio galactose (Gal) to arabinose (Ara) in the cell envelopes of EMB-treated cells. The detected variations in the compositions of fatty acids indicate that both the cell wall skeleton and outer layer (free lipids) are decomposed due to EMB treatment.

Postselective [directed] mutagenesis of fast-growing strain of Mycobacterium vaccae as a method of creating mutants showing changed phenotypic properties

84%

Sajduda A. , Dziadek J. , Golanska E. , Rumijowska A. , Jaworski A.

Acta Microbiologica Polonica

1994

tom 43

nr 2

Enhancement of beta-sitosterol transformation in Mycobacterium vaccae with increased cell wall permeability

84%

Korycka-Machala M. , Rumijowska-Galewicz A. , Lisowska K. , Ziolkowski A. , Sedlaczek L.

Acta Microbiologica Polonica

2001