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EN
Spring frosts can cause very significant losses on fruit farms. The most common method of active protection against frost is sprinkling. The aim of this study was to assess the effectiveness of a sprinkling system used to protect apple trees against the effects of a spring frost. The experiment was conducted on the Mączewscy Fruit Farm. The orchard in which the observations were made is equipped with a system of sprinklers. When the temperature readings showed values close to zero, the sprinkler system was activated. Due to a limited water supply, one row of sprinklers had not been activated, and thus some of the trees were not sprayed at all. The assessment of fruit yield carried out in the autumn of 2015 demonstrated high effectiveness of the sprinkling used to protect apple trees against the spring frost. The frost had damaged most of the flowers of the unprotected trees, which resulted in a decrease in yield of as much as 85-93%.
EN
The effects of bacterial and fungal inocula on the growth, yielding, and nutritional status of apple trees was evaluated in 3-years experiment (2018 - 2020). The experiment included the following treatments: (i) control (unfertilized soil), (ii) no fertilization + soil application of fungi, (iii) no fertilization + soil application of bacteria. The mixture of beneficial fungi contained two species: Aspergillus niger and Purpureocillium lilacinum. The mixture of beneficial bacteria contained three strains of Bacillus (Bacillus sp., Bacillus amyloliquefaciens and Paenibacillus polymyxa). The application of beneficial microorganisms (especially bacterial strains) to the soil (without additional mineral fertilization) enhanced the growth of the apple trees. In the third year of the study (2020), the trees grown in the plots inoculated with bacteria bloomed the most intensively. Plant nutritional status (expressed as concentrations of elements in leaves) was not affected by the application of the bacterial strains or filamentous fungi. The stronger growth of trees in the plots where the bacteria were used was likely related not so much to the nutritional status of the trees, but to the mitigation of the influence of the negative factors that cause the replant disease.
PL
W latach 2005-2009 badano zdolność kiełkowania stratyfikowanych nasion jabłoni otrzymanych z programów krzyżowań wykonanych w latach 2004-2008. Łącznie było to 121 kombinacji zapyleń, z użyciem 28 form rodzicielskich. Nasiona uzyskane z programów krzyżowań odkażano 0,1% roztworem fungicydu Benlate (poprzez zamoczenie na 24 godz.) i poddawano stratyfikacji. Stratyfikacja polegała na jednoczesnym wysiewie wszystkich nasion do małych doniczek plastykowych (tace wielokomórkowe) o wymiarach 5,5 cm x 5,5 cm i pojemności 90 cm3, napełnionych mieszaniną substratu torfowego, ziemi kompostowej i piasku w stosunku 1:1:1 (1 nasiono do 1 doniczki) i umieszczeniu ich na 30 dni w zwykłej chłodni o temperaturze 1-2°C. Po tym okresie doniczki z wysianymi nasionami ustawiono na parapecie w szklarni w celu kiełkowania, zapewniając sztuczne doświetlanie (16 godz.) i temperaturę ok. 20°C. Generalnie najlepiej kiełkowały nasiona z programu krzyżowań wykonanego w 2005 roku, średnio 81%, przy czym najwięcej skiełkowanych nasion stwierdzono w rodzinach: ‘Free Redstar’ x ‘Retina’ (89%), ‘Free Redstar’ x ‘Szampion’ (87%) i ‘Free Redstar’ x ‘Pinova’ (85%), natomiast najsłabiej kiełkowały nasiona z programu krzyżowań wykonanego w roku 2007 (średnio 14%, w rodzinie ‘Free Redstar’ x ‘Ligol’ (12%)) i w roku 2006 (średnio 22%, w rodzinach: ‘Ligolina’ x ‘Szampion’ (13%), ‘Ligolina’ x ‘Ligolina’ (17%), ‘Ariwa’ x J-79 (18%)).
EN
Germination of stratified apple seeds received from the crossings of 28 parental forms (121 cross-combinations) performed at the Research Institute of Pomology and Floriculture, Skierniewice, Poland in 2004-2008 was evaluated. Prior to stratification the seeds were disinfected with Benlate (0.1%) by soaking for 24 hours. Stratified seeds were placed individually into cells of the plastic multipots (5.5 cm x 5.5 cm) filled with the mixture of peat substrate, compost soil and sand (1:1:1). For stratification the multipots with seeds were placed in cold storage at temperature of 1-2°C for 30 days to undergo the stratification process. After this time, the containers were transferred into greenhouse at 20°C with artificial illumination (16 h). Seeds germination was evaluated every second day. The greatest number of seeds germinated was stated in 2005 (on average 81%) and the best germination was found for three cross-combinations - ʻFree Redstar’ x ʻRetina’ (89%), ʻFree Redstar’ x ʻSzampion’ (87%) and ʻFree Redstar’ x ʻPinova’ (85%). The weakest was seeds’ germination in 2007 (on average 14%) with only 12% of germinated seeds for the family ‘Free Redstar’ x ‘Ligol’.
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EN
The aim of the present study was to estimate the relationship between the crop load, number of fruits produced per unit of trunk cross-sectional area (TCA), the average fruit weight and the yield of 'Jonagold' apple trees. The trees apple (Malus domestica Borkh. cv. 'Jonagold') grafted on P 60 rootstock were planted at 3.5 x 1.25 m spacing. In order to achieve a high diversity of fruit load, light (L) as well as heavy (H) thinning were applied. The fruitlets were not thinned in the control (Control) trees. The yield and average fruit weight depended on the applied thinning. The correlation coefficient between crop density (CD) and yield was positive, whereas that between CD and average fruit weight was negative. The mean fruit weight was influenced not only by the number of fruit per unit of trunk cross- sectional area but also by the age of a tree.
PL
Celem badań było określenie zależności pomiędzy intensywnością owocowania wyra żoną liczbą owoców przypadaj ących na cm2 powierzchni przekroju poprzecznego pnia a plonowaniem oraz średnią masą owoców jabłoni odmiany Jonagold. Jesienią 1992 roku drzewa zaszczepione na podkładce P 60 posadzono w rozstawie 3,5 x 1,25 m w Sadowniczym Zakładzie Doświadczalnym w Dąbrowicach. Dla osiągnięcia dużego zróżnicowania zagęszczenia owocowania zastosowano dwa poziomy przerzedzania zawiązków oraz nieprzerzedzaną kontrolę. Otrzymane wyniki wykazują istotną dodatnią korelację pomiędzy poziomem WGO a plonowaniem drzew i ujemną korelacj ę pomiędzy tym wskaźnikiem a średnią masą owoców. Dla szczegółowego opisania tych zależności wyznaczono parametry ich równań liniowych. Wielkość średniej masy owoców uzależniona była nie tylko od intensywności owocowania, lecz tak że od wieku drzew.
EN
To investigate the photoinhibition of photosynthesis in ‘Honeycrisp’ apple (Malus domestica Borkh. cv. Gala) leaves with zonal chlorosis, we compared pigments, CO₂ assimilation and chlorophyll (Chl) a fluorescence (OJIP) transient between chlorotic leaves and normal ones. Chl and carotenoids (Car) contents, Chl a/b ratio, and absorptance were lower in chlorotic leaves than in normal ones, whereas Car/Chl ratio was higher in the former. Although CO₂ assimilation and stomatal conductance were lower in chlorotic leaves, intercellular CO₂ concentration did not differ significantly between the two leaf types. Compared with normal leaves, chlorotic ones had increased deactivation of oxygen-evolving complexes (OEC), minimum fluorescence (Fₒ), dissipated energy, relative variable fluorescence at L-, W-, J- and I-steps, and decreased maximum fluorescence (Fm), maximum quantum yield for primary photochemistry (Fv/Fm or φRₒ/ABS), quantum yield for electron transport (ETₒ/ABS), quantum yield for the reduction of end acceptors of photosystem I (PSI) (uRo and REₒ/ABS), maximum amplitude of IP phase, amount of active photosystem II (PSII) reaction centers (RCs) per cross section (CS) and total performance index (PItot,abs). In conclusion, photoinhibition occurs at both the donor (i.e., the OEC) and the acceptor sides of PSII in chlorotic leaves. The acceptor side is damaged more severely than the donor side, which possibly is the consequence of over-reduction of PSII due to the slowdown of Calvin cycle. In addition to decreasing light absorptance by lowering Chl level, energy dissipation is enhanced to protect chlorotic leaves from photo-oxidative damage.
EN
Apple species and cultivars differ in nuclear (2C) DNA content and ploidy level. The majority of these genotypes are diploids, but there are some triploids and a few tetraploids. Nuclear DNA content is a specific feature and its flow cytometric evaluation can be helpful in differentiating taxa. For many apple genotypes – including all the Polish ones, these characteristics are not known. 2C DNA was evaluated in relation to leaf, flower, fruit, pollen grain and stomata sizes as well as to the flowering time for seventy genotypes (including 46 Polish cultivars) gathered in the gene bank of the Research Institute of Horticulture, Skierniewice, Poland. For standard cultivars with the known chromosome number, 2C value was 1.71 pg for diploid cultivar ‘Alwa’ (2n=2x=34), 2.55 pg for triploid ‘Boskoop’ (3x=51), and 3.37 pg for tetraploid genome (4x=68) of mixoploid ‘McIntosh 2x+4x’. In 61 cultivars (including 41 Polish ones), the nuclear DNA content ranged from 1.58 to 1.78 pg indicating their diploid chromosome number. Five cultivars were identified as triploids (‘Bursztówka Polska’, ‘Pagacz’, ‘Rapa Zielona’, ‘Rarytas Śląski’ and ‘Witos’) owing to their nuclear DNA amount ranging between 2.42 and 2.58 pg. Leaf, flower, fruit, stomata and pollen grain sizes were on average significantly larger in triploids. Thus, in 3x plants the mean leaf surface was 49.1 cm², flower diameter – 52.4 mm, fruit weight – 204.7 g, stomata length – 32.1 μm and pollen grain diameter – 33.7 μm, whereas in diploids – 36.0 cm², 46.1 mm, 162.7 g, 28.4 μm and 30.7 μm, respectively. Pollen grain viability was on average significantly higher in diploids (75.6%), compared to triploids (22%). These results confirm that in apple, as in many other plant species, the higher ploidy level of triploids is generally associated with increased sizes of pollen grains, stomata, flowers, fruits and leaves but decreased pollen viability. No clear correlation between ploidy level and flowering time was found. In the case of mixoploid apple genotypes possessing diploid and tetraploid genomes, some phenotype observation is helpful in describing the ploidy level of the histogenic layers, L1 and L2. Small stomata sizes (similar to diploid) indicate diploid L1 and larger leaf sizes, compared to diploid counterparts, show tetraploid L2. The results will be used for breeding, in which it is important to determine maternal and paternal genotypes as well as the direction of the crossing that is of great importance in obtaining seeds and materials for further selection.
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