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1

Classic techniques for improvement of industrial yeast strains: Part III ? A method for enucleation of Saccharomyces sp.

100%

Salek A. T.

Biotechnologia

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2002

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nr 1

187-194

EN

The first method for enucleation of yeast Saccharomyces cerevisiae is reported. Various strains, including some killer strain and respiratory-deficient mutants of Saccharomyces cerevisiae were enucleated after treatment with cytochalasin B. Removal of nuclei from protruding sphaeroplasts was induced by centrifugation in a Percoll density gradient. The enucleation yield (which averaged about 80%) and the quality of the cytoplasts were best when the yeast culture had been synchronized with nocodazole before the preparation. The presence of 1 mM CaCl2 and ATP (10 muM) in the enucleation medium prevented the formation of fragile products or aggregation. Cytoplasts could be stored for at least 1 day without visible deterioration.

2

The selection of mosaic (MSC) phenotype after passage of cucumber (Cucumis sativus L.) through cell culture ? a method to obtain plant mitochondrial mutants

100%

Bartoszewski G. , Havey M. , Ziolkowska A. , Dlugosz M. , Malepszy S.

Journal of Applied Genetics

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2007

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tom 48

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nr 3

1-9

EN

Mosaic (MSC) mutants of cucumber (Cucumis sativus L.) appear after passage through cell cultures. The MSC phenotype shows paternal transmission and is associated with mitochondrial DNA rearrangements. This review describes the origins and phenotypes of independently produced MSC mutants of cucumber, including current knowledge on their mitochondrial DNA rearrangements, and similarities of MSC with other plant mitochondrial mutants. Finally we propose that passage of cucumber through cell culture can be used as a unique and efficient method to generate mitochondrial mutants of a higher plant in a highly homozygous nuclear background.

3

The selection of mosaic (MSC) phenotype after passage of cucumber (Cucumis sativus L.) through cell culture ? a method to obtain plant mitochondrial mutants

100%

Bartoszewski G. , Havey M. J. , Ziolkowska A. , Dlugosz M. , Malepszy S.

Journal of Applied Genetics

|

2007

|

tom 48

|

nr 1

1-9

EN

Mosaic (MSC) mutants of cucumber (Cucumis sativus L.) appear after passage through cell cultures. The MSC phenotype shows paternal transmission and is associated with mitochondrial DNA rearrangements. This review describes the origins and phenotypes of independently produced MSC mutants of cucumber, including current knowledge on their mitochondrial DNA rearrangements, and similarities of MSC with other plant mitochondrial mutants. Finally we propose that passage of cucumber through cell culture can be used as a unique and efficient method to generate mitochondrial mutants of a higher plant in a highly homozygous nuclear background.