Wyniki wyszukiwania - Biblioteka Nauki

Ten serwis zostanie wyłączony 2025-02-11.

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

2 Journal of Applied Genetics

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: LEAF RUST

Sortuj według:

Ogranicz wyniki do:

PstIAFLP based markers for leaf rust resistance genes in common wheat

100%

Blaszczyk L. , Tyrka M. , Chelkowski J.

nr 4

357-364

The aim of the present study was to detect candidate DNA markers for selected leaf rust resistance genes. A total number of 286 loci in the ?Thatcher' near-isogenic lines carrying resistance gene Lr1, Lr9, Lr10, Lr13, Lr19, Lr21, Lr24, Lr26, Lr28, Lr35, and Lr37 were screened for DNA polymorphism by the PstIAFLP method. A survey with 33 selective primers yielded 16 candidate markers. Further validation studies on cultivars characterized for the presence and absence of selected resistance genes confirmed specificity of markers for Lr24, Lr26 and Lr37. The AFLP-based marker P42-530 was successfully converted into an STS marker. The new marker was linked with the Lr37-specific marker (CslVrga13) at the distance of 1.7 cM. The PstIAFLP method was found to be effective in the identification of DNA changes induced in hexaploid wheat by translocations from Agropyron elongatum, Secale cereale and Aegilops ventricosa.

Response to stripe rust (Puccinia striiformis Westend. f. sp. tritici) and its coincidence with leaf rust resistance in hexaploid introgressive triticale lines with Triticum monococcum genes

100%

Sodkiewicz W. , Strzembicka A. , Sodkiewicz T. , Majewska M.

nr 3

205-211

Triticale introgressive lines were developed by incorporating diploid wheat (Triticum monococcum [TM16]) genes into the hexaploid triticale genotype LT522/6. The synthetic allotetraploid T. monococcum cereale (AmAmRR) was used as a bridging form to introduce the genes. A group of 43 introgressive lines, parental stocks and a check cultivar were inoculated at the seedling stage (in the greenhouse) and at the adult plant stage (in the field) with four pathotypes of Puccinia striiformis f. sp. tritici to determine if the stripe rust resistance was derived from TM16 and to analyze the expression of the diploid wheat gene(s) at the hexaploid level. At the seedling stage, 14 triticale introgressive lines expressed resistance to some of the used pathotypes, showing introduction of a genetic material from the T. monococcum genome. Among them, 7 lines were resistant to all four stripe rust pathotypes applied at this stage. In the field, adult plant resistance and percentage of infected leaf area were scored and transformed into the coefficient of infection. Plant response to stripe rust was compatible at these two developmental stages with a high statistical probability showing the genetic dependence on the same genetic background. Also observed was a full concordance of the adult plant resistance to stripe rust with previously assessed resistance to leaf rust, as well as the highly significant linkage of the resistance to the both diseases at the seedling stage in the set of the tested introgression lines. This result strongly suggests that T. monococcum genes responsible for these characters are located in proximity.