Wyniki wyszukiwania - Biblioteka Nauki

1

The effect of thiocetam on the parameters of the nitric oxide system under the conditions of the experimental periodontitis and immobilization stress formation

100%

Regeda M. , Olekshij P. , Regeda-Furdychko M. , Furdychko L. , Kolishetska M. , Regeda S. , Fil V.

|

nr 2

340-346

EN

Introduction and aim. The aim of this work is to study the parameters of the nitric oxide (NO) system in the blood of guinea pigs under the conditions of the experimental periodontitis (EP) and immobilization stress (IS) formation and to evaluate the effectiveness of thiocetam use. Material and methods. Experimental studies were performed on 50 guinea pigs (males, body weight 0.18–0.21 kg) which were divided into five groups (10 in each): the first group were intact animals as control; the second experimental group were animals with experimental periodontitis under conditions of immobilization stress (3rd day), the third group included guinea pigs with EP and IS on the 5th day of the combined model process, group IV – animals with EP and IS 15th day (without administration of thiocetam) and group V – animals on the 15th day of experiment with EP and IS after use of thiocetam. Results. As a result of this research, changes in the activity of the NO system in the blood were observed, namely an increase in the level of stable metabolites and an increase in the activity of total NO-synthase, which is accompanied by a compensatory inhibition of the L-arginine activity, and these indicators were most pronounced in the late stages of EP and IS formation. Conclusion. The use of thiocetam showed a corrective effect on the changed variables of NO metabolism in the peripheral blood of guinea pigs under the conditions of the EP and IS development.

2

Development and validation of a TLC-densitometric method for the simultaneous quantitation of icariin and L-arginine from commercial formulations

100%

Gupta P. K. , Kuber V. V. , Ghosh V. K. , Bhope S. G. , Sharma V. , Purohit S.

|

2011

|

tom Vol. 23, no. 3

447--458

EN

A simple, rapid, and specific thin layer chromatographic (TLC) method has been developed and validated for the simultaneous estimation of icariin and L-arginine from commercial polyherbal formulations for sexual dysfunction. The separation of the methanol extract of these formulations was achieved on silica gel 60 F254 aluminum backed TLC plates by using ethyl acetate-acetone-glacial acetic acid-formic acid-water 12:2:1:2:2 (υ/υ) as mobile phase. Densitometric analysis of icariin and L-arginine was monitored in absorbance mode at 270 and 195 nm, respectively. The linear regression analysis data for the calibration plots for icariin and L-arginine showed good linear relationship with r2 = 0.9984 +- 0.01 and 0.9968 +- 0.02, in the concentration ranges of 250–750 and 500–1500 ng/spot, respectively. The method was validated for precision, robustness, and recovery. The average percentage recovery was found to be 98.26% for icariin and 99.63% for L-arginine. The limits of detection and quantitation were 72, 116 and 238, 383 ng/spot, respectively, for icariin and L-arginine. Statistical analysis proves that the method is repeatable and selective for the estimation of the targeted drugs. Since the proposed mobile phase effectively resolves the icariin and L-arginine, this method can be applied for the identification and quantitation of these components in herbal extracts and marketed formulations.

3

Dietary vitamin E and selenium yeast supplemented with L-arginine enhanced laying performance and egg quality attributes of guinea fowl (Numida meleagris)

86%

Johnson A. A. , Oso A. O. , John M. O. , Adedeji A. S. , Ibrahim A. O. , Adeniji O. A. , Sulyman A. , Adetona A. , Ajibade F. P.

Animal Science and Genetics

|

2022

|

tom 18

|

nr 4

4

Nitric oxide mediates antinociceptive effect of leucopyrokinin active analog [2-8]-leucopyrokinin [[2-8]-LPK]

86%

Rykaczewska-Czerwinska M. , Konopinska D. , Plech A.

|

2005

|

nr 4

65-70

5

Duodenocutaneous fistula in rats as a model for "wound healing-therapy" in ulcer healing: the effect of pentadecapeptide BPC 157, L-nitro-arginine methyl ester and L-arginine

86%

Skorjanec S. , Kokot A. , Drmic D. , Radic B. , Sever M. , Klicek R. , Kolenc D. , Zenko A. , Lovric Bencic M. , Belosic Halle Z. , Situm A. , Zivanovic Posilovic G. , Masnec S. , Suran J. , Aralica G. , Seiwerth S. , Sikiric P.

Journal of Physiology and Pharmacology

|

2015

|

tom 66

|

nr 4

6

Enhanced expression of endothelial nitric oxide synthase in the myocardium ameliorates the progression of left ventricular hypertrophy in L-arginine treated Wistar-Kyoto rats

86%

Ahmad A. , Sattar M. A. , Rathore H. A. , Abdulla M. H. , Khan S. A. , Abdullah N. A. , Johns E. J.

|

nr 1

7

Immunohistochemical evaluation of caspase 3 expression in rats' hepatocytes after L-arginine therapy

86%

Pedrycz A. , Kot K. , Olesinski I.

Bulletin of the Veterinary Institute in Puławy

|

2010

|

tom 54

|

nr 1

101-103

EN

The apoptotical effect of nitric oxide on effector apoptotical caspase 3 in rats' hepatocytes was examined. The experiment was performed on 16 white Wistar female rats divided into two equal groups. The rats from the experimental group received orally L-arginine in a dose of 40 mg/kg b.w. every other day for 2 weeks. The rats from the control group received orally 2 ml of distilled water in the same manner as the experimental group. All the rats were decapitated after 3 weeks of the experiment. After decapitation, specimens from the liver were collected, fixed in 10% formalin, and then embedded in paraffin blocks. Protein caspase 3 on slides was detected using the standard three-step immunohistochemical method. The quantitative evaluation of caspase 3 expression showed that the area occupied by positive caspase 3 reaction in the liver of the experimental group (128.11 µm²±96.54) was comparable to that in the control group (212.18 µm² ±1 16.59) (P=0.25). The dose of L-arginine used was similar to that applied in pregnant women treated for gestosis. The study shows that L-arginine as a donor of exogenous nitric oxide has no an apoptotic effect on rats' hepatocytes.

8

Effect of L-arginine on lead induced oxidative stress in the blood of rats with different resistance to hypoxia

86%

Tkachenko H. , Kurhalyuk N. , Khabrovska L. , Kminski P.

|

nr 3

387-393

EN

The aim of the study was to estimate beneficial effects of L-arginine, a nitric oxide precursor, on antioxidant enzymes activity (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, ceruloplasmine), the lipid peroxidation processes level and parameters of membrane erythrocytes resistance before and after lead intoxication in rats with different resistance to hypoxia. Our results suggest that the antioxidant system enzymes activity and lipid peroxidation processes level in animals which differ in sensitiveness to hypoxia, are higher in animals with low resistance to hypoxia in the control group. We have shown that the amino acid, L-arginine, is an efficient antioxidant capable of reducing the level of lipid peroxidation processes in blood of lead-preexposed rats. L-arginine treatment under lead intoxication caused alteration in antioxidant enzymes activity due to increasing the enzymes activity of glutathione system, especially in animals with low resistance to hypoxia. The influence of L-arginine under lead intoxication was investigated to ascertain whether this amino acid possesses antioxidant properties before lead injection (preventive effect) and whether L-arginine has therapeutic effects by treatment after lead intoxication. We have shown a significant protective effect of L-arginine under treatment with a preventive effect before lead intoxication. These studies suggest that L-arginine may be a useful drug in treatment under lead intoxication.

PL

Zamierzeniem pracy było określenie korzystnych efektów działania L-argininy, prekursora tlenku azotu, na aktywność enzymów antyoksydacyjnych (dysmutazy ponadtlenkowej, katalazy, glutationreduktazy, glutationperoksydazy, ceruloplazminy), intensywność procesów peroksydacji lipidów i parametry odporności błony erytrocytów, przed i po intoksykacji ołowiem, u szczurów z różną odpornością na niedotlenienie. Nasze wyniki sugerują, że aktywność enzymów antyoksydacyjnych i poziom procesów lipoperoksydacji, u zwierząt różniących się wrażliwością na niedotlenienie, są wyższe u osobników z niską odpornością na niedotlenienie w grupie kontrolnej. Wykazaliśmy, że aminokwas L-arginina jest efektywnym antyoksydantem, zdolnym do zmniejszania intensywności procesów lipoperoksydacji we krwi szczurów eksponowanych na ołów. Traktowanie L-argininą w warunkach intoksykacji ołowiem powoduje zmianę aktywności enzymów antyoksydacyjnych, spowodowanym wzrostem aktywności enzymów systemu glutationu, w szczególności u zwierząt z niską odpornością na niedotlenienie. Wpływ L-argininy w warunkach intoksykacji ołowiem zbadaliśmy w celu określenia, czy aminokwas ten determinuje właściwości antyoksydacyjne przed intoksykacją ołowiem (efekt prewencyjny) oraz, czy L-arginina wykazuje efekty terapeutyczne w wyniku jej oddziaływania po intoksykacji ołowiem. Wykazaliśmy znaczący rezultat ochronny L-argininy w warunkach działania efektu prewencyjnego przed intoksykacją ołowiem. Nasze badania sugerują, że L-arginina może być użytecznym lekiem w warunkach intoksykacji ołowiem.

9

Mitochondria recycle nitrite back to the bioregulator nitric monoxide

86%

Nohl H. , Staniek K. , Sobhian B. , Bahrami S. , Redl H. , Kozlov A. V.

|

2000

|

tom 47

|

nr 4

EN

Nitric monoxide (NO) exerts a great variety of physiological functions. L-Arginine supplies amino groups which are transformed to NO in various NO-synthase-active isoenzyme complexes. NO-synthesis is stimulated under various conditions increasing the tissue of stable NO-metabolites. The major oxidation product found is nitrite. Elevated nitrite levels were reported to exist in a variety of diseases including HIV, reperfusion injury and hypovolemic shock. Denitrifying bacteria such as Paracoccus denitrificans have a membrane bound set of cytochromes (cyt cd1, cyt bc) which were shown to be involved in nitrite reduction activities. Mammalian mitochondria have similar cytochromes which form part of the respiratory chain. Like in bacteria quinols are used as reductants of these types of cytochromes. The observation of one-e- divergence from this redox-couple to external dioxygen made us to study whether this site of the respiratory chain may also recycle nitrite back to its bioactive form NO. Thus, the aim of the present study was therefore to confirm the existence of a reductive pathway which reestablishes the existence of the bioregulator NO from its main metabolite NO2-. Our results show that respiring mitochondria readily reduce added nitrite to NO which was made visible by nitrosylation of deoxyhemoglobin. The adduct gives characteristic triplet-ESR-signals. Using inhibitors of the respiratory chain for chemical sequestration of respiratory segments we were able to identify the site where nitrite is reduced. The results confirm the ubiquinone/cyt bc1 couple as the reductant site where nitrite is recycled. The high affinity of NO to the heme-iron of cytochrome oxidase will result in an impairment of mitochondrial energy-production. "Nitrite tolerance" of angina pectoris patients using NO-donors may be explained in that way.

10

Flavonoids and nitric oxide synthase

86%

Olszanecki R. , Gebska A. , Kozlovski V. I. , Gryglewski R. J.

Journal of Physiology and Pharmacology

|

2002

|

tom 53

|

nr 4,1

EN

Induction of NOS-2 in macrophages and smooth muscles within vascular wall with concomittant suppression of endothelial NOS-3 activity is considered to be a hallmark of vascular inflammation that triggers atherogenesis. Accordingly, drugs designed to reverse these changes should not only support vaning function of NOS-3 but also suppress proinflammatory NO production by NOS-2. It means that using selective inhibitors of induction of NOS-2 (they spare ex definitione constitutive activity of NOS-3) is a more rational approach than using isselectivel. inhibitors of activity of previously induced NOS-2. First of all, those drugs are never sufficiently selective. In our work we tried to identify inhibitors of NOS-2 induction within the group of flavonoids, known stimulators of NOS-3 with putative antiatherogenic effects. Representatives of four main groups of flavonoids: flavonols (kaempferol, quercetin, rutin), flavones (apigenin, primuletin), flavanols (catechine) and flavanones (hesperetin, hesperidin, naringenin) were tried on NOS-2 induction and activity in the in vitro model of LPS-treated macrophages (cell line J774.2). While none of these compounds inhibited activity of NOS-2, all with unexpectedly scattered potencies inhibited induction of NOS-2 protein in LPS-treated J774.2 cells, as evidenced by Western blotting technique. Subsequently, RT-PCR and Northern blotting methods revealed that so far the most potent compounds, kaempferol and apigenin, at micromolar concentrations did inhibit NOS-2 induction at the level of NOS-2 gene transcription. We conclude that some of flavonoids are potent inhibitors of NOS-2 induction. At the same time they may increase endothelial NOS-3 activity. Could these flavonoids become natural parents of future drugs, which will be used for reversal of inflammatory component of atherothrombosis?

11

Biochemical targets of nitric oxide-induced toxicity

86%

Grudzinski I. P.

|

nr 1

EN

Nitric oxide (NO) has become one of the most intensively studied molecules in recent years. Although its beneficial role has been well established, a large body of adverse effects was also attributed to NO and/or its red-ox derivatives in biological systems. Peroxynitrite (ONO-), a product of reaction between NO and superoxide anion (O2•-) was recognized as a potent pro-oxidant endogenous toxicant. The agent was found to induce DNA and protein oxidative damages leading to increased risk(s) of severe human pathologies including cancer. In this review, the discrete chemical aspects of both nitric oxide and peroxynitrite have been discussed in an attempt to elucidate the major biochemical target(s) of NO-and/or peroxynitrite-induced toxicity.

12

Carboxyebselen a potent and selective inhibitor of endothelial nitric oxide synthase

86%

Hatchett R. J. , Gryglewski R. J. , Mlochowski J. , Zembowicz A. , Radziszewski W.

Journal of Physiology and Pharmacology

|

1994

|

tom 45

|

nr 1

13

Administration of L-arginine stimulates oxygen consumption during hypoxia in mice

86%

Lach H. , Srebro Z. , Sura P. , Mindur I.

Acta Biologica Cracoviensia. Series Zoologia

|

2001

|

tom 43

14

An endogenous defensive concept, renewed cytoprotection/adaptive cytoprotection: intra(per)-oral/intragastric administration of strong alcohol in rat. Involvement of pentadecapeptide BPC 157 and nitric oxide system

72%

Becejac T. , Cesarec V. , Drmic D. , Hirsl D. , Madzarac G. , Djakovic Z. , Bunjevac I. , Zenko Sever A. , Sepac A. , Batelja Vuletic L. , Stancic Rokotov D. , Seiwerth S. , Sikiric P.

Journal of Physiology and Pharmacology

|

2018

|

tom 69

|

nr 3

15

Nitric oxide (NO) : a universal regulator of an organism’s vital processes at the cellular level

72%

Pedrycz A. , Siermontowski P. , Ciechan A. , Orłowski M. , Zając G.

Polish Hyperbaric Research

|

2013

|

tom nr 2(43)

93--103

EN

At the present time, seven types of nitrogen oxide have been discovered and defined: Nitrogen oxide (I) (N2O) – nitrous oxide, also known as a laughing gas, Nitric oxide (II) (NO) – nitrogen monoxide, Nitrogen oxide (III) (N2O3), Nitrogen oxide (IV) (NO2) – nitrogen dioxide which may produce a dimer – N2O4 – dinitrogen tetroxide, Nitrogen oxide (V) (N2O5) – dinitrogen pentoxide, Nitrogen oxide (VI) (NO3) nitrogen trioxide – nitrate radical of a strong oxidizing effect, Nitrogen oxide (VII) (N2O6) – dinitrogen hexoxide – an unstable compound with a peroxide bond O2N-O-ONO2. Of the above compounds the one that is of the greatest significance from the point of view of medicine is nitric oxide (NO), hence it is the main focus of this work.

PL

Do chwili obecnej odkryto i opisano 7 tlenków azotu: Tlenek azotu (I) (N2O)- podtlenek azotu, tlenek di azotu, inaczej zwany gazem rozweselającym, Tlenek azotu (II) (NO) - monotlenek azotu, Tlenek azotu (III) (N2O3), Tlenek azotu IV (NO2) - dwutlenek azotu, który może tworzyć dimer- N2O 4 - czterotlenek azotu, Tlenek azotu (V) (N2O5) pięciotlenek di azotu, Tlenek azotu (VI) (NO3) trójtlenek azotu- rodnik azotanowy o silnym działaniu utleniającym, Tlenek azotu (VII) (N2O6)- sześciotlenek azotu - nietrwały związek z wiązaniem nadtlenkowym O2N-O-O-NO2. W medycynie największe znaczenie ma monotlenek azotu (NO) i jemu poświęcona jest niniejsza praca.

16

Immunohistochemical assessment of the effects of L-arginine as a nitric oxide [NO] substrate on caspase 3 expression in rats' renal tubular cells

72%

Pedrycz A. , Boratynski Z. , Krasowski A.

Bulletin of the Veterinary Institute in Puławy

|

2010

|

tom 54

|

nr 3

EN

The study was performed on 16 albino Wistar female rats divided into two equal groups: experimental and control. The rats from the experimental group received per os, every second day for 2 weeks 40 mg/kg b.w. of L-arginine. The rats from the control group received, in the same manner, 2 ml of distilled water. The animals were decapitated after 3 weeks of the experiment. After decapitation specimens from the kidneys were collected, fixed in 10% formalin, and then embedded in paraffin blocks. Protein caspase 3 was detected using the standard three step immunohistochemical method. Additionally, the apoptotic index was evaluated. The study shows that L-arginine, as a donor of exogenous nitric oxide, induced the apoptotic signal in normal renal tubular cells of the rats. The apoptotic index statistically significantly increased in the epithelial cells of the treated renal tubules compared to the control. The immunohistochemical reaction for the executing caspase 3 in the renal tubular cells, although increased in comparison with the control, was statistically insignificant.

17

The mechanism of L-arginine oxidation by quinquevalent vanadium in the presence of cationic surfactant in sulphuric acid media. A kinetic study

72%

Dubey S. , Pandey A.

Bulletin of the Polish Academy of Sciences. Chemistry

|

2001

|

tom Vol. 49, No. 2

183-191

EN

The oxidation is first order each in [substrate] and the [oxidant]. The reaction follows an acid catalysed path, exhibiting second order dependence in [H+]. Bunnett's plot indicates that water acts as a nucleophile. Protonated form of oxidant has been postulated as the reacting oxidizing species. The rate studied at four different temperatures enabled us to calculate activation parameters. A suitable mechanism has been proposed.

18

Ontogeny regulates creatine metabolism in rat small and large intestine

72%

Garcia-Miranda P. , Garcia-Delgado M. , Peral M. J. , Calonge M. L. , Ilundain A. A.

Journal of Physiology and Pharmacology

|

2009

|

tom 60

|

nr 3

127-133

EN

The ontogeny of intestinal CRT, AGAT and GAMT was investigated in foetuses, newborn, suckling, weaning and adult rats. In the colon, CRT mediates creatine transport because it was Na+- and Cl- dependent and inhibited by creatine and GPA. In addition, Northern assays showed two CRT transcripts (2.7-kb and 4.2-kb) and the in situ hybridisation revealed that CRT mRNA is restricted to the colon epithelial cells. The immunohistochemistry revealed that CRT protein was at the apical membrane of colon epithelia. Maturation decreased colonic CRT activity to undetectable levels and increased CRT mRNA abundance. Western assays revealed 57-, 65-, 80- and 116-kDa polypeptides at the intestinal apical membrane. The abundance of the 65-, 80- and 116-kDa polypeptides decreased with age, and that of 57-kDa was only observed in adult rats. The small and large intestine express AGAT and GAMT mRNAs. Maturation decreased AGAT mRNA abundance without affecting that of GAMT. For comparison, renal AGAT mRNA levels were measured and they were increased with age. The study reports for the first time that: i) the apical membrane of rat colon have an active CRT, ii) development down-regulates CRT activity via post-transcriptional mechanism(s), iii) the intestine might synthesize creatine and iv) intestinal and renal creatine synthesis is ontogenically regulated at the level of AGAT gene expression.

19

Effect of chlorfenvinphos on the liver and plasma arginase activity in rats

72%

Maciejewska-Kozak H. , Szczepaniak S.

Acta Poloniae Toxicologica

|

1996

|

tom 04

|

nr 2

20

L-arginine decreases heat shock protein 70 [marker of environmental stress] expression in kidney cells of rat fetuses during apoptosis - late effect of adriamycin action

72%

Pedrycz A. , Brzeski Z.

|

tom 13

|

nr 1

EN

Both Adriamycin and nitric oxide (NO) cause apoptosis acting through or as free radicals inciting oxidative stress in the cell. However, in some tissues the antiapoptotic action of NO was described, thereby the impact of NO on cell apoptosis is not finally recognized. In this study, a trial of the evaluation of exogenous NO (L-arginine) impact on apoptosis induced by Adriamycin in fetal kidney cells was undertaken. For this reason, the expression of Heat Shock Protein 70 (HSP 70), environmental stress marker, as a sensitive biomarker of oxidative stress induced in fetal kidney cells with Adriamycin given to mothers prior to pregnancy was studied using immunohistochemical method. The expression of HSP 70 in fetal kidney cells, whose mothers received apart from Adriamycin, L-arginine (as NO substrate) was also evaluated. The results of the study pointed to the fact that the exogenous NO (L-arginine) could be helpful in inhibition of intensified apoptosis in fetal cells as a late effect of Adriamycin action.