Wyniki wyszukiwania - Biblioteka Nauki

1

Daptomycin-resistant Staphylococcus pettenkoferi of human origin

100%

Savini V. , Kosecka M. , Siegwart E. , Marrollo R. , Polilli E. , Palmieri D. , Fazii P. , Carretto E. , Międzobrodzki J. , Bukowski M.

Acta Biochimica Polonica

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2016

|

tom 63

|

nr 2

297-301

EN

The importance of nosocomial infections caused by coagulase-negative staphylococci is constantly growing. The threat primarily affects immunocompromised patients, the elderly and neonates, particularly after invasive surgery. The problem is fundamentally exacerbated by expanding antibacterial drug resistance. A case report is presented of an 86-year-old patient who underwent a ruptured abdominal aortic aneurysm surgery and developed septicaemia upon surgical wound infection. The causal agent was likely a carbapenem-resistant Klebsiella pneumoniae, however, daptomycin-resistant Staphylococcus pettenkoferi was identified in blood cultures in the absence of daptomycin treatment. To the authors' knowledge, the case study presented is the first published episode of daptomycin-resistant S. pettenkoferi strain.

2

Nosocomial outbreak due to Klebsiella pneumoniae at a neurosurgery unit

100%

Sekowska A. , Kaminska D. , Nakonowska B. , Gospodarek E.

Polish Journal of Microbiology

|

2012

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tom 61

|

nr 4

EN

Thirty three isolates of K. pneumoniae were studied. The strains were cultured from different clinical specimens received from patients hospitalised at a Neurosurgery Unit at the Dr Jurasz University Hospital in Bydgoszcz. Production of ESBL was assessed using double disk synergy test. The genomic DNA was extracted from the strains separated by PFGE after digesting with XbaI endonuclease. Production of ESBL was detected in 81.8% of K. pneumoniae isolates. Molecular typing results revealed a great genetic diversity among K. pneumoniae isolates. All repeated PFGE patterns were detected in 12 (36.3%) K. pneumoniae isolates.

3

Klebsiella pneumoniae: characteristics of carbapenem resistance and virulence factors

100%

Candan E. , Aksöz N.

Acta Biochimica Polonica

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2015

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tom 62

|

nr 4

867-874

EN

Klebsiella pneumoniae, known as a major threat to public health, is the most common factor of nosocomial and community acquired infections. In this study, 50 K. pneumoniae clinical specimens isolated from bronchial, urea, blood, catheter, rectal, bile, tracheal and wound cultures were collected. These isolates were identified and carbapenem resistance was determined via an automated system, CHROMagar Orientation and CHROMagar KPC. The carbapenemase gene regions (blaIMP, blaVIM, blaOXA, blaNDM and blaKPC) and presence of virulence factors (magA, k2A, rmpA, wabG, uge, allS, entB, ycfM, kpn, wcaG, fimH, mrkD, iutA, iroN, hly ve cnf-1) of these isolates were determined by using Multiplex-PCR. The OXA-48 carbapenemase gene regions were determined in 33 of 50 K. pneumoniae strains. In addition, NDM-1 resistance in one, OXA-48 and NDM-1 resistance in four unusual K. pneumoniae isolates were detected. Virulence gene regions that were encountered among K. pneumoniae isolates were 88% wabG, 86% uge, 80% ycfM and 72% entB, related with capsule, capsule lipoprotein and external membrane protein, responsible for enterobactin production, respectively. Even though there was no significant difference between resistant and sensitive strains due to the virulence gene regions (P≥0.05), virulence factors in carbapenem resistant isolates were found to be more diverse. This study is important for both, to prevent the spread of carbapenem resistant infections and to plan for developing effective treatments. Moreover, this study is the first detailed study of the carbapenem resistance and virulence factors in K. pneumoniae strains.

4

Wrazliwosc szczepow Klebsiella pneumoniae na srodki dezynfekcyjne

89%

Janowska J.

Roczniki Państwowego Zakładu Higieny

|

1999

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tom 50

|

nr 2

219-224

PL

Porównano wrażliwość na środki dezynfekcyjne szczepów Klebsiella pneumoniae: wyizolowanego ze środowiska szpitalnego (Ks) i pochodzącego z muzeum szczepów PZH (K28) z wrażliwością testowego szczepu E. coli NCTC 8196. Zbadano działanie 7 preparatów dezynfekcyjnych. Szczepy Klebsiella wykazały większą wrażliwość na środki dezynfekcyjne niż E. coli. Jedynie w przypadku formaliny zależność ta była odwrotna.

EN

The sensitivity of 2 Klebsiella strains (isolated from hospital environment - Ks and museum K28) to 7 disinfectants with the sensitivity of referent strain E. coli NCTC 8196 were compared. Suspension method was applied. Determined the sensitivity Klebsiella strains for phenol, septyl, lizol, chloramine, formalin, glutaraldehyde and laurosept in compare with sensitivity of E. coli during 10 minutes of exposure. Certify the insignificant of difference in testing sensitivity of both Klebsiella strains on the majority disinfectants and more sensitive those strains than referent strain E. coli. In the case of chloraminę the difference was almost two fold - the value concentration ratio of the solutions giving bactericidal effect for E. coli in comparing the some concentration for Ks was 2.3. Only in the case of formalin the sensitivity of E. coli and Klebsiella pneumoniae was inverse the value of concentration ratio was 0.51 - E. coli strain was 1.9 more sensitive than Ks strain and 1.6 more sensitive than K28 strain.

5

Wrazliwosc na wybrane antybiotyki beta-laktamowe klinicznych szczepow Escherichia coli i Klebsiella pneumoniae

88%

Andrzejewska E. , Szkaradkiewicz A. , Kaniasty M.

Medycyna Doświadczalna i Mikrobiologia

|

1998

|

tom 50

|

nr 3-4

197-205

PL

W pracy dokonano analizy lekooporności szczepów Escherichia coli i Klebsiella pneumoniae izolowanych od pacjentów. Wykonano testy określające zdolność bakterii do produkcji ß-laktamaz o rozszerzonym spektrum substratowym (ESBL).

EN

The studies aimed at analysing the resistance to some ß-lactam antibiotics among E. coli and K. pneumoniae clinical isolates and at evaluating. The extended spectrum of ß-lactamases (ESBL) production in the isolates. The analysis included 137 E. coli strains and 52 K. pneumoniae strains, isolated from hospitalized patients and out-patients treated in the first trimester of 1998. The strains were identified using the ATB computer system. Antibiotic sensitivity of the isolates was determined by disc-diffusion tests. ESBL production capacity of E. coli and K. pneumoniae strains was estimated by double-disc and ATB BLSA tests. Most of the analysed E. coli strains were found to exhibit significant sensitivity to compound penicillin preparations containing ß-lactarn inhibitor (Augmentin, Tazocin) and to the third generation cefalosporins, in contrast, K. pneumoniae strains much more frequently were resistant to the drugs. Among the obtained isolates, 3 (2,2%) E. coli strains and 21 (40,4%) K. pneumoniae strains produced ESBL but all the isolates proved sensitive to imipenem. In evaluation of ESBL production-detecting tests, the double-disc test was found to be more reliable than ATB BLSA test.

6

Genetyczne podobienstwo szczepow Klebsiella pneumoniae izolowanych z materialu klinicznego

88%

Sekowska A. , Kaminska D. , Gospodarek E.

Medycyna Doświadczalna i Mikrobiologia

|

2009

|

tom 61

|

nr 3

215-220

7

Antibacterial activity of iron oxide nanoparticles synthesized by co-precipitation technology against Bacillus cereus and Klebsiella pneumoniae

88%

Ansari S. A. , Oves M. , Satar R. , Khan A. , Ahmad S. I. , Jafri M. A. , Zaidi S. K. , Algahtani M. H.

Polish Journal of Chemical Technology

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2017

|

tom Vol. 19, nr 4

110--115

EN

The present study investigates the synthesis and characterization of iron oxide nanoparticles (Fe3 O4 -NPs) for their antibacterial potential against Bacillus cereus and Klebsiella pneumonia by modified disc diffusion and broth agar dilution methods. DLS and XRD results revealed the average size of synthesized Fe3 O4 -NPs as 24 nm while XPS measurement exhibited the spin-orbit peak of Fe 2p3/2 binding energy at 511 eV. Fe3 O4 -NPs inhibited the growth of K. pneumoniae and B. cereus in both liquid and soild agar media, and displayed 26 mm and 22 mm zone of inhibitions, respectively. MIC of Fe3 O4 -NPs was found to be 5 μg/mL against these strains. However, MBC for these strains was observed at 40 μg/mL concentration of Fe3 O4 -NPs for exhibiting 40–50% loss in viable bacterial cells and 80 μg/mL concentration of Fe3 O4 -NPs acted as bactericidal for causing 90–99% loss in viability. Hence, these nanoparticles can be explored for their additional antimicrobial and biomedical applications.

8

Hemolityczna aktywnosc paleczek Klebsiella pneumoniae i Klebsiella oxytoca

75%

Szramka B. , Kurlenda J. , Bielawski K.

Medycyna Doświadczalna i Mikrobiologia

|

1998

|

tom 50

|

nr 3-4

207-213

PL

Niniejsza praca przedstawia wyniki badań nad aktywnością hemolityczną wybranych szczepów Klebsiella pneumoniae i Klebsiella oxytoca. Badane szczepy wykazywały aktywność hemolityczną specyficzną wobec erytrocytów króliczych. Hemoliza występowała tylko po inkubacji hodowli z ß-merkaptoetanolem lub chlorkiem wapnia.

EN

We demonstrated that Klebsiella pneumoniae and Klebsiella oxytoca possess a selective haemolytic activity on rabbit erythrocytes. Thirty one Klebsiella strains (18 strains of K. pneumoniae and 13 strains of K. oxytoca) were isolated from hospitalized patients. The liquid (Trypcase-soy broth - TSB) and solid (Trypcase-soy agar - TSA) medium, containing the red cells were used for the tests. All the screened strains showed a haemolytic effect on rabbit erythrocytes, provided that the supernatants of the cultures were preincubated with ß-mercaptoethanol or calcium chloride. There was no human and sheep erythrocyte lysis.

9

Sanfetrinem, przedstawiciel trinemow - aktywnosc in vitro wobec szczepow Klebsiella pneumoniae wytwarzajacych ESBL

75%

Wieczorek P. , Sacha P. T. , Leszczynska K. , Jakoniuk P. , Zalewska M.

Medycyna Doświadczalna i Mikrobiologia

|

2006

|

tom 58

|

nr 1

27-32

PL

Zbadano wrażliwość 43 szczepów Klebsiella pneumoniae wytwarzających ESBL wobec sanfetrinemu. Uzyskane wyniki porównano z wynikami dla imi- penemu (MIC50, MIC90). Wszystkie izolaty były wrażliwe na imipenem i cefe- pim, a oporne na gentamycynę, amikacynę i trimetoprim/sulfametoksazol.

EN

Sanfetrinem is the first member of tricyclic ß-!actams (trinems) which can be administered orally as a hexatil ester. His chemical structure is related to carbapenems. High stability to many ß-lactamases and human renal dehydropeptydase were described. The investigation was performed on 43 strains of Klebsiella pneumoniae producing ESBL isolated from hospitalized patients. The MICs of sanfetrinem and imipenem were determined by E-test. Additio- naly, susceptibility to antibiotics was tested by disc diffusion method. Klebsiella pneumoniae ATCC 700603, Escherichia coli ATCC 25922 and Escherichia coli ATCC 35218 were used as a control strains. MIC50 and MIC90 of sanfetrinem and imipenem amounted respectively 0,38/3 mg/l and 0,19/0,25 mg/l. Range of MIC value was from 0,064 mg/l to 4 mg/l for sanfetrinem, and from 0,094 mg/l to 0,38 mg/l for imipenem. Additionaly, geometric and aritmetic means were calculated to both antibiotics. All results of study were compared using correlation factor and „Student" t-test. None of these 43 Klebsiella pneumoniae strains was resistant to imipenem and cefepime. Majority of isolates demonstrated susceptibility to ciprofloxacin and cefoxitin - 90,7% and 74,4% respectively. All strains were resistant to gentamicin, amikacin and trimethoprim/sulfamethoxazole.

10

Antibacterial activity of (PVP-ZrO2) nanocomposite against pathogenic bacteria

75%

Abdulrazaq R. A. , Al-Ramadhan Z. A. , Khalaf H. H.

World News of Natural Sciences

|

2018

|

tom 18

|

nr 2

187-194

EN

The antibacterial activity of a PVP-ZrO2 nanocomposite was investigated against pathogenic bacteria S. aureus and K. pneumoniae after antibacterial sensitivity was determined and one isolate was chosen that showed more antibiotic resistance. Herein, the Co-culture technique was used to calculate percent reduction of bacteria. The results that were obtained in this method show that ZrO2 nanoparticles have inhibitory effect against pathogenic bacteria gram negative bacteria and gram positive bacteria - with reduction of growth reaching 100% to both S. aureus and K. pnumoniae at 5, 10, 15, 20 and 25% ZrO2, compared with control. The resistance patterns of S. aureus and K.pnuemonia isolates show the Moxifloxacin (MXF) is the best antibiotic for both bacteria - with sensitivity at 100%, while resistance to Ceftriaxone (CRO) is at 90% S. aureus, and at 80% K. pnumoniae. The polymer-nanocomposite was prepared by weight percentage wt. % of (PVP) being dissolved in (10) ml of distilled water, with weight percentages 5%, 10%, 15%, 20% and 25% of ZrO2 nanoparticles added.

11

Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: control of an outbreak using a new ADSRRS technique

75%

Krawczyk B. , Samet A. , Czarniak E. , Szczapa J. , Kur J.

Polish Journal of Microbiology

|

2005

|

tom 54

|

nr 2

EN

Extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae (EPKP) strains are frequently implicated in outbreaks in neonatal units. From April 2002 to January 2003, 149 neonates were colonized/infected with EPKP In the Neonatal Clinic of the Teaching Hospital at the Medical University of Gdansk, Poland. A novel assay based on suppression of PCR, ADSRRS-fingerprinting, was successfully evaluated for typing EPKP isolates. The results showed that the genotypes of all outbreak-related strains were identical, which suggested that the outbreak originated from a single clone. This conclusion was confirmed by using different methods - RAPD and PFGE. The outbreak was stopped by adopting improved hygiene and instituting outbreak control measures.

12

Characteristics of Klebsiella pneumoniae harboring QnrB32, Aac(6’)-Ib-cr, GyrA and CTX-M-22 genes

63%

Wang D. , Wang H. , Qi Y. , Liang Y. , Zhang J. , Yu L.

Folia Histochemica et Cytobiologica

|

2012

|

tom 50

|

nr 1

13

Wystepowanie i charakterystyka powierzchniowego bialka P-like paleczek Klebsiella

63%

Przondo-Mordarska A. , Smutnicka D. , Matusiewicz K.

Medycyna Doświadczalna i Mikrobiologia

|

2003

|

tom 55

|

nr 2

135-146

PL

Właściwości nowo opisanej adhezyny P-like Klebsiella badano metodami hemaglutynacyjnymi, mikroskopowo-elektronowymi i biochemicznymi. Stwierdzono amorficzny charakter adhezyny i jej swoistość receptorową zbliżoną do adhezyny PapG E.coli.

EN

Colonisation and remaining of microorganism on mucus membrane of microorganism is tightly connected with adhesion mechanisms and determine the first step of physiological settlement of the organism or the first stage of clinically demonstrated infection. In Klebsiella rods there are known three types of fimbrial adhesins (type 1, 3 and KPF-28) and non-fimbrial adhesin CF29K. It is stated that Klebsiella strains adhesions are responsible for their adherence to the epithelial cells of both respiratory and urinary tracts and to intestine epithelium. The in vitro research affirmed Klebsiella rods adherence to protein matrix. The aim of our work was the establishment of character, receptor specifity and the appearance frequency of P-like called adhesin. The frequency of expression of P-like adhesin was estimated among 380 isolated from the patients strains on the basis of agglutinating methods. The amorphic character of P-like adhesin was proved using electron microscopy method. The isolation and purification of P-like protein with a help of affinity chromatography enabled to estimate the receptor specifity of the adhesin. The receptor specifity was established as similar to E.coli PapG adhesin.

14

Polimorfizm profili jednoniciowego DNA [SSCP] rejonu locus cps wspolnego paleczkom Klebsiella pneumoniae u szczepow epidemicznych i przypadkowo izolowanych

63%

Gierczynski R. , Kaluzewski S. , Zasada A. A. , Rastawicki W. , Jagielski M.

Medycyna Doświadczalna i Mikrobiologia

|

2005

|

tom 57

|

nr 2

153-161

PL

Określono profile SSCP wybranych fragmentów (ORF: 1,2,3 i 15) locus cps odpowiedzialnego za wytwarzanie otoczki typu K2 u szczepów K. pneumoniae izolowanych z materialu klinicznego od niemowląt. Wśród 72 badanych szczepów wyróżniono 31 genotypów z czego 11 stwierdzono u 12 szczepów przypadkowo izolowanych podczas gdy szczepy epidemiczne pochodzące z jednego ogniska charakteryzowały się przynależnością do tego samego genotypu. W grupie 54 szczepów epidemicznych wyosobnionych w sześciu ogniskach zakażeń szpitalnych wyodrębniono 15 genotypów, z których większość reprezentowała jedno spośród dwóch głównych odgałęzień dendrogramu.

EN

The SSCP of ORFs 1, 2, 3, 15 from the cps region for capsule biosynthesis was determined for 56 epidemic isolates, 4 reference strains of K. pneumoniae including A5054 and B5055, and 12 strains isolated casually from stool samples. From 6 up to 14 different SSCP-profiles were observed for tested loci, which combined together distinguished 31 SSCP-genotypes. Epidemic strains could be diversified into 15 genotypes whereas 11 genotypes were detected for 12 casual isolates. Strains from the same outbreak belonged to a single genotype. Strains from different outbreaks represented separate genotypes, however majority of them was located in the same main branch of a dendrogram based on the cluster analysis of the SSCP-profiles diversity. Obtained results may suggest high genetic diversity of tested loci. The SSCP genotyping of multiple cps loci was found as potentially useful tool for tracing epidemic strains during an outbreak

15

Występowanie i chorobotwórczość szczepów Enterobacteriaceae – Klebsiella Pneumoniae wytwarzających karbapenemazę klasy b (mbl/ndm) u pacjentów hospitalizowanych i leczonych w mazowieckim szpitalu specjalistycznym w radomiu w latach 2016 – 2018.

63%

Ura L. , Deja-Makara B. , Pajdziński M. , Gottwald L. M.

Long-Term Care Nursing

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2020

|

tom 5

|

nr 3

239-249

EN

Aim The aim of the study was to characterize symptomatic infections and asymptomatic carrier of class B carbapenemase (MBL/NDM) produced Klebsiella pneumoniae in patients hospitalized and treated in Mazowiecki Memorial Hospital of Radom between 2016-2018. Material and methods The study group included 120 patients aged 80+/ – 17.0 years old. Epidemiologic analysis of the study group was conducted. Results The number of patients positive towards Klebsiella pneumoniae MBL was: 57 (47,5%) in 2016, 32 (26,6%) in 2017 and 31 (25,8%) in 2018. The study group included 44 women (36,67%) and 76 men (63,33%) (p<0,005). In clinically significant material 60 bacterial strains were identified, with similar frequency from lower respiratory tract discharge and from urine. Symptomatic infections in 60 patients (50%) and asyptomatic carrier in 60 patients (50%) were confirmed. Symptomatic infections from lower respiratory tract were more frequent than urinary tract infections (p=0,037), wound infections (p<0,001), generalized infections (p<0,001) and digestive tract infections (p<0,001). During 2016-2018 reduction in sensitivity of Klebsiella pneumoniae MBL strains for colistin (100%; 46,5%; 45,8%) and amikacin (72,8%; 80,8%; 45,8%) was observed. Conclusions 1. In hospitalized patients positive towards Klebsiella pneumoniae MBL asymptomatic carrier and symptomatic infections occured with similar frequency. 2. In hospitalized patients Klebsiella pneumoniae MBL strains were most frequently identified from respiratory tract and from urinary tract. 3. Commonly with elongation of periode, then colistin and amikacin were used, the reduction of bacterial sensitivity for these antibiotics was observed.

PL

Cel pracy Celem pracy była charakterystyka objawowych zakażeń i bezobjawowego nosicielstwa szczepow Klebsiella pneumoniae wytwarzających karbapenemazę klasy B (MBL/NDM) u pacjentow leczonych na oddziałach szpitalnych Mazowieckiego Szpitala Specjalistycznego (MSS) w Radomiu w latach 2016-2018. Materiał i metody Grupę badaną stanowiło 120 pacjentow w wieku 80 lat +/ – 17,0. Dokonano analizy epidemiologicznej tych przypadkow. Wyniki Stwierdzono 57 przypadkow (47,5%) w 2016 r., 32 (26,6%) w 2017 r. i 31 (25,8%) w 2018 r. pacjentow z obecnością Klebsiella pneumoniae MBL. W grupie badanej były 44 kobiety (36,67%) i 76 mężczyzn (63,33%) (p<0,005). Z materiałow istotnych klinicznie wyhodowano łącznie 60 szczepow Klebsiella pneumoniae MBL. Z podobną częstością izolowano je z wydzieliny z dolnych drog oddechowych oraz z moczu (p=0,436). U 60 pacjentow (50%) stwierdzono zakażenie objawowe, a w 60 przypadkach (50%) wystąpiło bezobjawowe nosicielstwo. Zakażenia dolnych drog oddechowych występowały częściej od zakażeń układu moczowego (p=0,037), zakażeń rany (p<0,001), zakażeń uogolnionych (p<0,001) oraz zakażeń układu pokarmowego (p<0,001). W latach 2016, 2017 i 2018 wykazano zmniejszenie się wrażliwości szczepow Klabsiella pneumoniae MBL na kolistynę (100%; 46,5%; 45,8%) i amikacynę (72,8%; 80,8%; 45,8%). Wnioski 1. Bezobjawowa kolonizacja pacjentow szczepami Klebsiella pneumoniae MBL występuje z podobną częstością do objawowych zakażeń spowodowanych przez te bakterie. 2. Szczepy Klebsiella pneumoniae MBL izolowane są najczęściej z drog oddechowych i moczowych hospitalizowanych pacjentow. 3. Wraz z wydłużeniem okresu stosowania kolistyny i amikacyny w grupie badanej zaobserwowano zmniejszenie wrażliwości szczepow Klebsiella pneumoniae MBL na te antybiotyki.

16

Characterization of Klebsiella pneumoniae strains isolated from urinary tract infection: Detection of ESBL characteristics, antibiotic susceptibility and RAPD genotyping

63%

Aladag M. O. , Uysal A. , Dundar N. , Durak Y. , Gunes E.

Polish Journal of Microbiology

|

2013

|

tom 62

|

nr 4

EN

In this study, a hundred Klebsiella pneumoniae strains isolated from urinary tract infections were evaluated in terms of genotyping, susceptibility to certain antibiotics and detection of extended spectrum of beta lactamase (ESBL) production. The random amplified polymorphic DNA (RAPD-PCR) method was used to identify the genetic differentiation of K pneumoniae isolates. A total of 26 different DNA bands ranging between 334 bp and 28033 bp were detected among the strains. It was found that 100 K. pneumoniae strains revealed 11 different RAPD profiles. Antibiotic susceptibility tests were conducted using a disc diffusion method against 16 antibiotics. Fifty-five different resistance profiles were determined among the strains. ESBL-productions of the strains were determined by the double disc synergy test (DDST) and ESBL E-test methods. ESBL production rates among the strains were found to be 55% by E-test method and 45% by DDST method. While ESBL-producing K. pneumoniae strains showed the greatest resistance to penicillin G (100%), followed by piperacillin (92.7%) and erythromycin (85.4%), the resistance rates of non ESBLproducing strains to those antibiotics were determined as 97.8%, 88.8% and 88.8%, respectively. Both groups of strains showed the highest sensitivity to meropenem. Based on the results obtained from the study, it was concluded that the detection of ESBL-producing strains by the E-test method was more sensitive than by the DDST method. Phenotypic and genotypic identification methods should be used together to detect ESBL presence. The RAPD-PCR method alone will not be adequate in the genotyping of the strains and alternative DNA-based methods should be used.

17

Antimicrobial susceptibility of metallo-beta-lactamase positive and negative Klebsiella pneumoniae strains isolated from intensive care unit patients

63%

Sekowska A. , Hryniewicz W. , Gniadkowski M. , Deptula A. , Kusza K. , Gospodarek E.

Polish Journal of Microbiology

|

2010

|

tom 59

|

nr 1

67-69

EN

Carbapenem resistance in Gram-negative bacteria is a worldwide increasing and one of the most disturbing problems, given these antibiotics are drugs of choice in the treatment of infections caused by extended-spectrum-beta-lactamase producing strains. In this study the antibiotic susceptibility of metallo-beta-lactamase-positive and negative Klebsiella pneumoniae strains isolated from intensive care unit (ICU) patients was evaluated. The presence of genes encoding MBLs was determined with a commercial kit hyplex® MBL ID (BAG HEALTH CARE). The MBL-producing isolates were the first K.pneumoniae isolates of this kind identified in Poland. It seems that methods for detecting MBLs in Enterobacteriaceae should be included in contemporary standards of microbiological diagnostics in the country.

18

Wykrywanie ekspresji beta-laktamaz o rozszerzonym spektrum substratowym [ESBL] wsrod klinicznych szczepow Klebsiella pneumoniae i Escherichia coli; porownanie E-testu i dwoch testow krazkowych

63%

Andrzejewska E. , Szkaradkiewicz A.

Medycyna Doświadczalna i Mikrobiologia

|

2004

|

tom 56

|

nr 2

161-171

PL

Stosując E-test i dwie techniki krążkowe - DDST i DD wykrywano ESBLs wśród 148 klinicznych szczepów E. coli i 78 szczepów K pneumoniae. Wykazano 100% zgodność wyników uzyskanych przy zastosowaniu w/w metod do wykrywania ESBLs wśród szczepów K pneumoniae. W wykrywaniu ESBLs wśród szczepów E. coli stwierdzono 100% zgodność wyników w E-teście i teście DD, natomiast 75% w metodzie DDST. Dokonano również analizy lekowrażliwości badanych szczepów bakteryjnych.

EN

The aim of this study was to compare the effectiveness of E-test and two disc methods applied for the detection of extended spectrum ß-lactamases. All strains were tested by E-test, by double-disc synergy test (DDST) according to Jarlier (cefotaxim, ceftazidim, aztreonam and clavulanic acid) and also by disc test according to Appleton (Cefpodoxime and Cefpodoxime with clavulanic acid, CPD and CD01 disc). We tested 148 clinical strains of E. coli and 78 strains of K. Pneumoniae. In case of K. pneumoniae, the activity of the ESBLs was detected among 30 strains — both in E-test, Jarlier test and Appleton test. Among E. coli, four strains were found ESBL-positive in the test according to Jarlier but only three strain of these when E-test and Appleton test was used. The results of investigations performed suggest, that E-test and disc methods according both Jarlier and Appleton have the same effectiveness in detection ESBLs among K. pneumoniae strains. However, in case of E. coli, interpretation of results may present a problem.

19

Charakterystyka opornych na fluorochinolony izolatów z gatunku K. pneumoniae, P. mirabilis i E. coli wytwarzających beta-laktamazy typu ESBL i AmpC

63%

Rzeczkowska M. , Piekarska K. , Gierczynski R.

Medycyna Doświadczalna i Mikrobiologia

|

2012

|

tom 64

|

nr 4

PL

Analizie poddano 19 losowo wybranych izolatów należących do trzech gatunków: K. pneumoniae (n=7), P. mirabilis (n=3) i E. coli (n=9), wyosobnionych z materiału klinicznego od chorych w jednym z warszawskich szpitali. Badano zróżnicowanie plazmidowo-kodowanych β–laktamaz typu ESBL i AmpC, wytwarzanych przez oporne na fluorochinolony izolaty należące do wymienionych gatunków. Mechanizm ESBL, który dominował głównie wśród przedstawicieli gatunku E. coli, wykryto w przypadku 9 izolatów. Nabyte β-laktamazy AmpC stwierdzono u 6 badanych izolatów, w tym wśród wszystkich izolatów z gatunku P. mirabilis. Ponadto, w przypadku 4 izolatów należących do gatunku K. pneumoniae wykryto zarówno mechanizm ESBL, jak i AmpC. W prezentowanych badaniach geny warunkujące wytwarzanie pAmpC najczęściej należały do rodziny CMY i DHA, zaś geny warunkujące wytwarzanie ESBL – do rodziny TEM, SHV i CTX-M. Ponadto, 13 spośród 19 badanych izolatów poza niewrażliwością na fluorochinolony i cefalosporyny trzeciej generacji charakteryzowało się również opornością na antybiotyki aminoglikozydowe i trimetoprim/sulafametoksazol. Uzyskane wyniki wskazują na horyzontalny transfer plazmidów, w których znajdują się geny warunkujące wytwarzanie β-laktamaz typu ESBL i/lub pAmpC.

EN

Introduction: Extended-spectrum β-lactamases (ESBLs) are the predominant mechanism for acquired antibiotic resistance in Enterobacteriaceae. During the past few years, increasing occurrence of plasmid-mediated AmpC β- lactamases (pAmpCs) particularly in K. pneumonia, P. mirabilis and E. coli was reported. Therefore, the aim of our study was to analyse of the diversity of plasmid-mediated β-lactamases such as pAmpCs and ESBLs among clinical K. pneumonia, P. mirabilis and E. coli strains in Poland. Methods: A total of 19 clinical Enterobacteriaceae strains (E. coli, n=9; K. pneumoniae, n=7; P. mirabilis, n=3) resistant to third-generation cephalosporin were selected from collection of fluoroquinolone resistant isolates recovered during a 6-months period in regular hospital in Warsaw, Poland. ESBLs and AmpCs were detected by using phenotypic methods: double-disc tests (DDSTs), MAST ID D68C test, sensitivity to cefoxitin, disk potentiation test (DPT) and Tris-EDTA test. Polymerase chain reaction (PCR) amplification of the blaAmpC, blaCTX-M, blaTEM, and blaSHV, genes. PCR-products for these genes were sequenced. To determine the possible clonality of the tested isolates PFGE with the XbaI was performed. Results: Nine of 19 fluoroquinolone-resistant strains tested produced extended-spectrum β-lactamases of TEM, SHV and CTX-M families. These ESBLs were most commonly detected in E. coli. AmpC β-lactamases were produced by 6 tested strains, including 3 isolates of P. mirabilis. The AmpC found in our study belonged to CMY and DHA families. Furthermore, 4 isolates of K. pneumoniae were found to co-produce both ESBL and AmpC β-lactamases. XbaI-PFGE profiles pointed significant differences of tested strains. Conclusion: Horizontal transfer of genes encoding for acquired β-lactamases such ESBL and AmpC seem to play primary role in dissemination of these resistance traits among fluoroquinolone-resistant clinical strains of Enterobacteriaceae in Poland.

20

Występowanie niektórych gatunków pałeczek gram-ujemnych z rodzajów Yersinia, Klebsiella, Aeromonas oraz ich rola w etiologii zatruć pokarmowych

63%

Maciejska-Roczan K.

Roczniki Państwowego Zakładu Higieny

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1979

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tom 30

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nr 3