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EN
Faecal coliform bacteria (among others Escherichia coli) are routinely used in monitoring of water areas (first of all seaside resorts). Their occurrence indicates recent pollution by municipal sewage. Survival of these bacteria in the sea water depends on the complex of biological, chemi-cal and physical water parameters. There are many conflicting opinions on bacterial death-rate and the data on this phenomenon for the Gulf of Gdansk are rather scarce. The influence of irradi-ance (0-200 mumol photon m-2 s-1), temperature (5-20 ?C) and salinity (0-7.5 psu) on the survival of Escherichia coli strain K-12 has been investigated. The bacterial cultures were incubated for three days under chosen temperature, salinity and irradiance conditions. Experiments were planned based on the factorial design (method x'). The cell density was determined every day by direct viable count on agar plates. The obtained results were analysed statistically in such a way that temperature, salinity and irradiance were independent variables whereas Escherichia coli number and mean mortality coefficient (Km) were the dependent ones. It was found that the effect of salinity (in studied range from 0 to 7.5 psu) on Escherichia coli survival was not statistically significant. However, the survival was strongly correlated with temperature and irradiance. Correlation coefficient (R) between temperature and irradiance and mean coefficient of Escherichia coli mortality (Km) equalled 0.801. This dependence can be described by a function, obtained from nonlinear regression analysis, Km = 6.466 - 0.195 t + 2.215?10-7 t2 i2. The function will be used for the development of a model of matter circulation and energy flow in the Gulf of Gdansk.
EN
Long-term storage of shoots or nodal buds in vitro, in conditions which retard their growth, may be an alternative or supplementary method in the protection of plant resources and in the production of planting material for elite mother stocks. In this paper we describe the most important factors which influence the survival and further behaviour of stored explants.
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