The flow-sheet of for biotechnological application has been presented in the paper. The relevant topics that concern problems of microbial or as well as separation of reaction system onto the fractions with different residence time distributions have been overiewed. A set of conditions has been specified for membranes applied in the system. The following types of bioreactors have been characterized in detail i.e.:i) case of bioreactor for hydrolysis of penicillinum G and ii) case of microbial membrane bioreactor for culture and harvesting of biomass iii) microbial membrane bioreactor for alcohol fermentation.
The effect of unilateral hindlimb immobilization and subsequent free remobilization on bone tissue in rats was examined. Right hindlimb of intensively growing (G), young adult (Y) and adult (A) male rats was immobilized by taping for two weeks. Bone tissue was investigated post mortem in experimental and age-matched control rats, either directly after immobilization (Imm) or after two or four weeks of remobilization (Re2, Re4). Apparent density (dapp) and mineralization (Min) were estimated in femora and pelvis. The mechanical state of bone tissue in femora was evaluated using an ultrasonic method. Additionally, activity of serum alkaline phosphatase, and serum calcium and phosphorus were measured in each group. Min and dapp in Imm bones were changed in G rats, while in Y and A only dapp in Imm femora was affected. Velocity of ultrasound was significantly lower in immobilized femora in each age group, indicating decreased elasticity of bone tissue. The differences between immobilized and control limbs were still significant in Re2 and Re4 groups in G rats. In Y rats the differences between experimental and control bones increased during remobilization. It is concluded that deterioration of bone initiated during two weeks of unloading, last within at least four weeks of free remobilization, despite restoration of normal activity.
Endolipases associated with the cell structures of microorganisms are more active in comparison with purified enzymes. Due to the weak mechanical resistance lipases immobilised in situ cannot be used many times. The method of lipases encapsulation in polysacharides hydrophilic gels was elaborated. The lipases Mucor immobilised in situ were treated with oleic acid in hexane and then dispersed in an aqueous solution of sodium alginate or karagenate. Immobilisation of enzymes was achieved by intermolecular cross-linking of the polysacharide chains using the solution of calcium or potassium salts. The biocatalysts prepared under proposed conditions were active in hydrolysis of esters, as well as in esterification reaction. It was found that immobilised enzymes were active for a long time, were mechanically resistant and could be used many times in periodic and continuous processes.
Art does not exist without myth. It is what gives meaning to an artwork. It is thinking in its own, and art, always impressing something on some- one, avoids strict, rational, but also prone to pressure norms path when we reach sacrum; art is the only language to speak about sacrum. The char- acter of Orpheus has inspired artists of different genres for centuries, but it seems to be seen from only one side. The mythical hero does not break a formal order, but an authentic norm, because he personifies a fact that norms do not exhaust human nature, only regulate life of societies. Thereby an “innocent” hero cannot be mythical. I searched for Orpheus’ guilt and Euridice’s counterparts in other myths, or even different mythologies. One of them is, then, Lot’s wife. I admit, I was inspired by Wisława Szymbor- ska’s poem about her.
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