Wyniki wyszukiwania - Biblioteka Nauki

1

The mechanism of interleukin-1 cytotoxicity in HeLa cells

100%

Doszczak M. , Pierzchalski A. , Holtmann H. , Bigda J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

2

On the spatial organization of ubiquitin-dependent proteolysis in HeLa cells

88%

Wojcik C.

Folia Histochemica et Cytobiologica

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1997

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tom 35

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nr 2

3

Pravastatin and simvastatin pretreatment in combination with pyrimethamine and sulfadiazine reduces infection process of Toxoplasma gondii tachyzoites (RH strain) in HeLa cells

75%

da Silva Sanfelice R. A. , da Silva S. S. , Bosqui L. R. , Machado L. F. , Miranda-Sapla M. M. , Panagio L. A. , Navarro I. T. , Conchon-Costa I. , Pavanelli W. R. , Almeida R. S. , Costa I. N.

Acta Parasitologica

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2019

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tom 64

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nr 3

4

Effect of extracts from Inonotus obliquus on the growth and enzyme activities of HeLa 83 cancer cells

75%

Rzymowska J. , Malarczyk E. , Jarosz A.

Applied Biology Communications. Lublin Scientific Center

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1991

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tom 01

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nr 3

5

Cytotoxic activity of ethyl-4-isothiocyanatobutanoate and its synthetic derivatives on HeLa cells

75%

Busanyova K. , Horakova K. , Repiska M. , Somorovska M. , Floch L.

Folia Histochemica et Cytobiologica. Supplement

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1997

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tom 35

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nr 2

6

Differential stability of mitochondrial mRNA in HeLa cells

75%

Piechota J. , Tomecki R. , Gewartowski K. , Szczesny R. , Dmochowska A. , Kudla M. , Dybczynska L. , Stepien P. P. , Bartnik E.

Acta Biochimica Polonica

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2006

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tom 53

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nr 1

EN

The physiological significance and metabolism of oligoadenylated and polyadenylated human mitochondrial mRNAs are not known to date. After study of eight mitochondrial transcripts (ND1, ND2, ND3, ND5, CO1, CO2, ATP6/8 and Cyt. b) we found a direct correlation between the half-lives of mitochondrial mRNAs and their steady-state levels. Investigation of the mt-mRNA decay after thiamphenicol treatment indicated that three transcripts (ND2, ND3 and Cyt. b) are significantly stabilized after inhibition of mitochondrial translation. Careful analysis one of them, ND3, showed that inaccurate processing of the H-strand RNA precursor may occasionally occur between the ND3 and tRNAArg locus leading to synthesis of ND3 mRNAs lacking the STOP codon. However, analysis of the oligo(A) fraction observed in case of the ND3 indicates that partially polyadenylated mRNAs are linked rather to the transcription process than to the translation-dependent deadenylation. Analysis of ND3 mRNA turnover in cells with siRNA-mediated knock-down of the mitochondrial poly(A) polymerase shows that strongly decreased polyadenylation does not markedly affect the decay of this transcript. We present a model where oligoadenylated mitochondrial transcripts are precursors of molecules containing full length poly(A) tails.

7

Both TNF receptors cooperate with interleukin 1 and induce the cytocidal effect in HeLa cells

75%

Bigda J. , Kozak M.

Folia Histochemica et Cytobiologica. Supplement

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1997

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tom 35

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nr 2

8

Quercetin suppresses heat shock-induced nuclear translocation of Hsp72

75%

Jakubowicz-Gil J. , Pawlikowska-Pawlega B. , Piersiak T. , Pawelec J. , Gawron A.

Folia Histochemica et Cytobiologica

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2005

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tom 43

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nr 3

9

Degradation of extracellular nucleotides and their analogs in HeLa and HUVEC cell cultures

75%

Gendaszewska-Darmach E. , Maszewska M. , Zaklos M. , Koziolkiewicz M.

Acta Biochimica Polonica

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2003

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tom 50

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nr 4

EN

The use of nucleotides and their analogs in the pharmacological studies of nucleotide receptors (P2 class) should be preceded by detailed studies on their degradation connected with ecto-enzymes of a given cell type. In the present studies we have analyzed stability of some phosphorothioate and phosphonate analogs of ATP and ADP in the HeLa epitheloid carcinoma and endothelial HUVEC cells cultures. Our studies have revealed that ecto-nucleotide pyrophosphatase (E-NPP) is one of the main enzymes involved in the extracellular degradation of ATP and other nucleotides in the HeLa cells. On the other hand, the ecto-ATPDase is responsible for the hydrolysis of extracellular nucleotides in human endothelial cell cultures, while the E-NPP-like enzymes of the HUVEC cells are not essential to this degradation. The concerted action of the aforementioned ecto-enzymes and nucleotide pyrophosphatase, 5 -nucleo- tidase and adenosine deaminase present in fetal bovine serum (FBS) supplied to the culture medium, results in partial or complete degradation of the phosphorothioate (ATPγS) and phosphonate analogs of adenosine nucleotides (α,β-methylene-ATP and β,γ-methylene-ATP) in the cell cultures. Only ADPβS appears to be resistant to these enzymes. The influence of some nucleotides and their analogs on the proliferation of the HeLa cells in presence or absence of FBS is also discussed.

10

Cell death in HeLa cells upon imperatorin and cisplatin treatment

63%

Jakubowicz-Gil J. , Paduch R. , Ulz Z. , Badziul D. , Glowniak K. , Gawron A.

Folia Histochemica et Cytobiologica

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2012

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tom 50

|

nr 3

11

Antimetastatic potentials of 8-aryl-2,6,7,8-tetrahydroimidazo [2,1-C] [1,2,4]triazine-3,4-diones

63%

Sztanke K. , Pasternak K. , Sztanke M. , Kandefer-Szerszen M.

Journal of Elementology

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2009

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tom 14

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nr 3 Suppl.

81-82

12

Overexpression of human CUTA isoform2 enhances the cytotoxicity of copper to HeLa cells

63%

Yang J. , Li Q. , Yang H. , Yan L. , Yang L. , Yu L.

Acta Biochimica Polonica

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2008

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tom 55

|

nr 2

411-415

EN

Copper, an essential transient element, can be toxic to cells when present in excess. Altered copper homeostasis is involved in pathological events of many diseases. Human CUTA isoform2 is a member of cation tolerance protein (CutA1) family. In this study, we examined the effect of CUTA isoform2 overexpression on copper toxicity. It was shown that overexpressed CUTA isoform2 sensitized HeLa cells to copper toxicity by promoting copper-induced apoptosis. The inhibition effect of excessive copper on cell proliferation was also enhanced by overexpressed CUTA isoform2. So CUTA isoform2 was implicated to be involved in the cytotoxicity of copper.

13

Cannabinoid receptor activation inhibits cell cycle progression by modulating 14-3-3beta

63%

Jung H.-W. , Park I. , Ghil S.

Cellular and Molecular Biology Letters

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2014

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tom 19

|

nr 3

EN

Cannabinoids display various pharmacological activities, including tumor regression, anti-inflammatory and neuroprotective effects. To investigate the molecular mechanisms underlying the pharmacological effects of cannabinoids, we used a yeast two-hybrid system to screen a mouse brain cDNA library for proteins interacting with type 1 cannabinoid receptor (CB1R). Using the intracellular loop 3 of CB1R as bait, we identified 14-3-3β as an interacting partner of CB1R and confirmed their interaction using affinity-binding assays. 14-3-3β has been reported to induce a cell cycle delay at the G2/M phase. We tested the effects of cannabinoids on cell cycle progression in HeLa cells synchronized using a double-thymidine block-and-release protocol and found an increase in the population of G2/M phase cells. We further found that CB1R activation augmented the interaction of 14-3-3β with Wee1 and Cdc25B, and promoted phosphorylation of Cdc2 at Tyr-15. These results suggest that cannabinoids induce cell cycle delay at the G2/M phase by activating 14-3-3β.

14

The lactate receptor (HCAR1/GPR81) contributes to doxorubicin chemoresistance via ABCB1 transporter up-regulation in human cervical cancer HeLa cells

63%

Wagner W. , Kania K. D. , Blauz A. , Ciszewski W. M.

Journal of Physiology and Pharmacology

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2017

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tom 68

|

nr 4

15

Inhibition of EGFR nuclear shuttling decreases irradiation resistance in HeLa cells

63%

Wei H. , Zhu Z. , Lu L.

Folia Histochemica et Cytobiologica

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2017

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tom 55

|

nr 2

16

Lunasin – a bioactive peptide from triticale (X Triticosecale Wittmack) seeds, inhibits proliferation of cancer HeLa and SK-OV-3 cells

63%

Galbas M. E. , Porzucek F. , Selwet M. , Nowak A. , Slomski R.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2017

|

tom 98

|

nr 3

17

Nucleases isolated from Chelidonium majus L. milky sap can induce apoptosis in human cervical carcinoma HeLa cells but not in Chinese Hamster Ovary CHO cells

63%

Nawrot R. , Wolun-Cholewa M. , Gozdzicka-Jozefiak A.

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tom 46

|

nr 1

79-83

18

Apoptosis of HeLa and CaSki cell lines incubated with all-trans retinoid acid

63%

Darmochwal-Kolarz D. , Gasowska-Giszczak U. , Paduch R. , Kolarz B. , Wilcinski P. , Oleszczuk J. , Kwasniewska A.

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2009

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tom 47

|

nr 4

19

DFF40-CAD hypersensitive sites are potentially involved in high molecular weight DNA fragmentation during apoptosis

63%

Widlak P.

Cellular and Molecular Biology Letters

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2000

|

tom 05

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nr 3

EN

Sequential cleavage of genomic DNA into large-scale DNA fragments of 50-300-kb, followed by formation of mono- and oligonucleosomal DNA fragments, is a biochemical hallmark of programmed, cell death (apoptosis). The endonuclease DFF40/CAD mediates regulated internucleosomal DNA fragmentation and chromatin condensation in cells undergoing apoptosis. DFF40 hypersensitive sites were detected in purified HeLa cell nuclei, and excision of 50-kb DNA fragments preceded formation of oligonucleosomal DNA ladders in nuclei treated with the nuclease. Topoisomerase II, but not topoisomerase I, stimulates DFF40 activity on plasmid DNA substrates. This suggests that interactions of DFF with the nuclear matrix-bound topoisomerase II may be involved in formation of DFF40 hypersensitive sites.

20

Highly fusogenic cationic liposomes transiently permeabilize the plasma membrane of HeLa cells

63%

Stebelska K. , Wyrozumska P. , Gubernator J. , Sikorski A. F.

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nr 1

EN

Cationic liposomes can efficiently carry nucleic acids into mammalian cells. This property is tightly connected with their ability to fuse with negatively charged natural membranes (i.e. the plasma membrane and endosomal membrane). We used FRET to monitor and compare the efficiency of lipid mixing of two liposomal preparations — one of short-chained diC14-amidine and one of long-chained unsaturated DOTAP — with the plasma membrane of HeLa cells. The diC14-amidine liposomes displayed a much higher susceptibility to lipid mixing with the target membranes. They disrupted the membrane integrity of the HeLa cells, as detected using the propidium iodide permeabilization test. Morphological changes were transient and essentially did not affect the viability of the HeLa cells. The diC14-amidine liposomes were much more effective at either inducing lipid mixing or facilitating transfection.