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1

Infertility and marker chromosomes: Application of molecular cytogenetic techniques in a case of inv dup(15)

100%

Vulcani-Freitas T. M. , Gil-da-Silva-Lopes V. L. , Varella-Garcia M. , Maciel-Guerra A. T.

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nr 1

89-91

EN

We report on a phenotypically normal man with infertility, whose 47,XY,+mar karyotype was studied by spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) using a chromosome-15-specific probe (LSI SNRPN). By these techniques, the marker chromosome was identified as a small inv dup (15). Possible causes for male infertility in this case are discussed.

2

Short-term fluctuations of chlorophyll alpha fluorescence versus diurnal variations of solar radiation in the surface water of the Gdansk Basin

100%

Magulski R. , Falkowska L. , Dunajska D. , Pryputniewicz D. , Sikorowicz S.

Oceanological and Hydrobiological Studies

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2004

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nr 3

57-68

EN

Measurements of chlorophyll alpha fluorescence accompanied by solar radiation measurements were carried out during two spring cruises in the region of the Gdansk Basin. Chlorophyll alpha fluorescence, similarly to that of chlorophyll alpha concentration in the seawater, showed considerable diurnal variability. The measurements provided statistically significant negative correlation coefficients that indicated that chlorophyll a fluorescence is inhibited as solar PAR and UV radiation intensity increases. The approximate range of radiation intensity that corresponded to a clearly marked decline in chlorophyll a fluorescence and chlorophyll alpha concentration was determined. It was revealed that the decline in chlorophyll alpha concentration in the surface water layer resulted from the photodegradation of chlorophyll alpha as well as phytoplankton grazing. In both cases, the resulting observation was an increase of pheophytine a concentration. The vertical migration of phytoplankton was identified as an additional process induced by high radiation levels.

3

Green fluorescent protein (GFP) an ecological transformation marker and a tool for observing processes in the living cell

100%

Sliwinska E.

Biotechnologia

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2002

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nr 1

129-135

EN

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has been extensively used as a marker of gene expression and protein targeting in intact cells and organisms. Since GFP has no toxic effect on cells and it is easy to detect, it can replace controversial transformation markers such as those for antibiotic or herbicide resistance. The protein can also be used to detect dynamic responses of signal transduction pathways, subcellular localisation of chimeric proteins, and transfection efficiency. It allows visualisation of the organelles and observation of many important cellular processes.

4

Estimation of mating-compatibility of the wild service tree (Sorbus torminalis L. Crantz) by observing pollen tube germination

100%

Wojciechowski A. , Bednorz B. L. , Bednorz L.

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nr 4

253-258

EN

Cross-compatbility and self-compatbility in trees of Sorbus torminalis (L.) Crantz were evaluated. Degree of mating-compatibility between three trees was determined by means of fluorescence microscope. Self-compatibility evaluated on the basis of the pollen tube germination index showed that one tree is fully self-incompatible and the other two are self-compatible. Observations of pollen tube germination showed full cross-compatibility between trees under investigation.

5

Tracking lymphocytes in vivo

100%

Adams C. L. , Kobets N. , Meiklejohn G. R. , Millington O. R. , Morton A. M. Y. , Rush C. M. , Smith K. M. , Garside P.

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2004

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tom 52

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nr 3

173-187

EN

The ability to track antigen (Ag)-specific lymphocyte populations in vivo has greatly increased our understanding of the location and functional status of these cells throughout the course of an immune response. Recent technical advances have enhanced researchers' capability to follow migration, activation and cellular interactions of Ag-specific lymphocytes in situ. It is now possible to monitor changes in T cell subsets, co-stimulatory molecules, and chemokine expression within the physiological context of secondary lymphoid organs. Furthermore, the Ag-presenting cell-T cell interaction can be studied, thus dissecting the role and timing of Ag presentation of particular dendritic cell subsets in the initiation of the immune response. The capacity to adoptively transfer small populations of Ag-specific T lymphocytes has also increased our knowledge of the physiologically important role of regulatory T cells in autoimmunity and immunosuppression. New fluorescence imaging techniques such as multicolor video microscopy, laser scanning cytometry, and multiphoton tissue imaging have provided new ways in which researchers can track cellular changes within Ag-specific lymphocytes in vivo. This review summarizes some of the ways in which these techniques have led to discoveries in the role of signaling cascades, cell cycle progression, and apoptosis in maintaining an Ag-specific immune response.

6

1;25 centric fusion recognition in the cattle chromosome by fluorescence in situ hybridization

100%

Ladon D. , Solinas-Toldo S. , Fries R. , Stranzinger G.

Journal of Applied Genetics

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1996

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tom 37

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nr 1

101-104

EN

Fluorescence in situ hybridization (FISH) experiments with specific probes for chromosome 29 and 25 were carried out on a Brown Swiss bull, previously diagnosed as a carrier of 1;29 centric fusion.The hybridization of the chromosome 29-specific probe (BMC 4216-already located on 29q13), produced signals on two small acrocentrics, but not the translocated chromosome.The signals appeared on the translocated chromosome and on a single chromosome 25 after hybridization of the chromosome-specific probe (BMC 3224 -previously located on 25q24).According to the actual nomenclature, the analysed aberration is a robertsonian translocation involving chromosomes 1 and 25.

7

Identification of three different chromosomal additions by chromosome painting using fluorescence in situ hybridization (FISH) technique

88%

Bocian E. , Stanczak H. , Obersztyn E. , Mazurczak T.

Journal of Applied Genetics

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1996

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tom 37

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nr 2

197-204

EN

Fluorescence in situ hybridization (FISH) is a very useful method for assessing chromosome rearrangements.When neither banding pattern nor clinical symptoms are sufficient to determine the origin of additoinal chromosomal fragments, FISH with multiple chromosome-specific libraries allows to solve this diagnostic problem rapidly.Three chromosomal additions, 7q+, 13p+ and 22 q+, found in routine cytogenetic studies performed in children with phenotypic abnormalities were analysed using FISH.This technique documented the origin of extra matrial to be derived from chromosome 16[der(7)t(7;16)(q36.3;p13.110], 18[der(13)t(13;18)(p12;q12.2)] and 22[dup(22)(q11.2q13.1)], repectively.In two cases the abnormality arose de novo, while in the third case the product of translocation t(13;18) was ,matrnal by origin.It was present in 30% of mother's lymphocytes, and in 70% of them a balanced Robertsonian translocation t(13q;15q) was found.In the present cases the chromosome analysis with both traditional banding and chromosome painting techniques, allowed to establish final clinical diagnosis.

8

Evaluation of crossability of Cuphea lanceolata and C.viscosissima based on pollen tube rowth and seed set

75%

Wojciechowski A. , Olejniczak J. , Adamska E.

Journal of Applied Genetics

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1996

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tom 37

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nr 3

277-384

EN

Many species of Cuphea genus show promise as rich natural sources of valuable seed oils for commercial utility.Among them are C.lancelota and C.viscosissima which seed oils contain mediumm-chain fatty acids.Recoprocal crosses were made between species to assess their usefulnes for Cuphea breeding programme.The degree of crossability between C.lanceolata and C.viscosissima was determinated by pollen grain germinatin and pollen tube growth observed using fluorescence microscopy in addition to capsule formation and seed number per capsule.Treatment of the pollen grain with a laser microbeam reduced the number of germinated pollen grains, tubes growth in the pistil and seed set when C viscisissima plants were used in the crosses as a female form.In the cross C.lanceolata (female form) x C viscosissima the pollen treatment with a laser microbeam improved pollen tube growth but reduced seed set.Crossability evaluated on the basis of pollen tube growth and seed set showed unilateral intraspecific incompatibility in crosses involving C.lanceolata plants as a female form.