Wyniki wyszukiwania - Biblioteka Nauki

1

Antibiotics resistance in Enterococcus isolates from poultry waste

100%

Cybulska K. , Krzyśko-Łupicka T.

Ecological Chemistry and Engineering. S

|

2020

|

tom Vol. 27, nr 2

305--316

EN

The aim of the study was to evaluate the drug resistance of Enterococcus faecalis and Enterococcus faecium isolated from different types of poultry waste. The study material consisted of feather samples (duck, turkey, chicken), sludge and centrifuge sediment, originating from three poultry farms. The study was conducted in two stages; isolation and identification of Enterococcus bacteria from the waste and evaluation of their drug resistance using Kirby-Bauer method. Contamination of the poultry waste with Enterococcus isolates included E. faecium species (79 %) and E. faecalis (21 %). The most contaminated were sludge and sediment from the centrifuge as well as chicken feathers, irrespective of the place and time of sampling. Tested isolates showed multiple resistance and similar reaction to all antibiotics used in the study and E. faecalis strain was more resistant. Enterococcus isolates showed the highest resistance to streptogramins, carbapenems, fluoroquinones, aminoglycosides and penicillins, and the lowest for nitrofurantions and phenicols.

2

Analysis of biofilm production in Enterococcus faecium strains depending on clinical source

99%

Sieńko A. , Wieczorek P. , Majewski P. , Sacha P. , Wieczorek A. , Ojdana D. , Tryniszewska E.

Progress in Health Sciences

|

2017

|

tom 7(1)

83-89

EN

Purpose: Enterococcus faecium strains have been reported worldwide as etiologic factors of many nosocomial infections, which are difficult to manage because of the constantly increasing resistance of these microorganisms to antibiotics and the ability to form biofilm. The aim of this study was to analyze the ability to produce a biofilm in E. faecium strains, depending on the patient’s clinical material. Materials and methods: Sixty-six E. faecium strains were investigated. Identification and susceptibility testing were conducted by the VITEK2 system. The ability to form biofilm was assessed by phenotypic methods. The presence of selected virulence genes was established by PCR followed by gel electrophoresis and sequencing. Results: Among the tested E. faecium isolates, 72.7% were biofilm-positive (BIO+) and 27.3% biofilm-negative (BIO-). Strains were collected mostly from rectal swabs (30.4%) and blood (18.3%). BIO+ strains from infections constituted 31.8% (52.4% isolated from blood) and from colonization 40.9% (48.2% from rectal swabs). 91.7% of the Blood Group strains and 68.5% of the Other Group strains produced biofilm. Strains from the Colonization Group produced biofilm in a proportion similar to the Infection Group (about 75%). There were no statistically significant differences in virulence and resistance, except for vancomycin (more resistant BIO+ Other than the BIO+ Blood Group, and more resistant BIO+ Colonization than BIO+ Infection Group) and teicoplanin (more resistant BIO+ Colonization than the BIO+ Infection Group). Conclusion: The majority of E. faecium isolates carries high levels of resistance to many antimicrobials, is well equipped with virulence genes, and possesses the ability to form biofilm.

3

Polymerase chain reaction detection of caecal bacteria in case of preventive application of Enterococcus faecium EK13 against Salmonella enterica subsp. Enteritidis in chickens

99%

Herich R. , Laukova A. , Strompfova V. , Revajova V. , Levkut M. , Pistl J.

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tom 14

|

nr 1

4

Isolation of faecal Enterococcus faecium strains from rabbits and their sensitivity to antibiotics and ability to bacteriocin production

99%

Simonova M. , Laukova A.

Bulletin of the Veterinary Institute in Puławy

|

2004

|

tom 48

|

nr 4

5

Zelazo niezbednym substratem pokarmowym enterokokow?

85%

Lisiecki P.

Medycyna Doświadczalna i Mikrobiologia

|

2010

|

tom 62

|

nr 3

271-280

PL

Żelazo jest substratem pokarmowym, którego enterokoki wymagają do wzrostu. Dowiedziono, że żelazo - bez względu na stężenie w pożywce - jest pobierane podczas wzrostu. Niedobór żelaza w pożywce powodował zmiany w kinetyce wzrostu enterokoków. Hamowanie wzrostu enterokoków przez chelatory żelaza jak i brak hamowania są kolejnym dowodem zapotrzebowania tych bakterii na żelazo. Enterokoki mają zdolność pobierania tego ważnego dla nich pierwiastka z jego połączeń z naturalnymi i syntetycznymi chelatorami o różnej sile wiązania i strukturze chemicznej.

EN

Enterococci were considered as not requiring iron. The aim of study was evaluation of relationship between enterococci and iron. This study examined these relationships in a 71 strains belonging to two species - Enterococcus faecalis and Enterococcus faecium, which are often isolated from human infections. The iron is an essential nutrient for enterococci. Demonstrated that iron - regardless of the concentration in the medium - is collected during growth. Iron deficiency in the nutrient medium resulted in changes in the kinetics of growth of enterococci. Inhibiting the growth of enterococci by iron chelators and lack of inhibition are further proof of this demand for iron bacteria. Enterococci have the ability to acquire this important element of its connections with natural and synthetic chetators with different strength of chemical bonding and structure. Bacteria of the genus Enterococcus have a natural resistance to many antimicrobial agents. In the hospital environment can easily acquire resistance genes to many other classes of antimicrobial compounds. For these reasons, treatment of enterococal infections poses more difficulties. Inhibition of iron uptake in enterococci can be helpful in reducing and combating enterococal infections.

6

Vancomycin-resistant Enterococcus faecium-associated encephalitis and concurrent cerebellitis

85%

Kang K. , Yoon S.-S. , Yoon B.-W.

Open Medicine

|

2011

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tom 6

|

nr 4

470-474

EN

Enterococci are uncommon etiologic agents of central nervous system infections. We describe a case of nosocomial encephalitis and concurrent cerebellitis associated with Enterococcus faecium in a man, with extranodal natural killer/T-cell lymphoma, nasal type, who underwent high-dose chemotherapy and autologous peripheral blood stem cell transplantation. Brain magnetic resonance images showed lesions in the bihemispheral cerebellar cortex with swelling and several small lesions in both cerebral hemispheres. The blood and cerebrospinal fluid cultures were positive for vancomycin-resistant E. faecium. Vancomycin-resistant E. faecium can cause encephalitis and concurrent cerebellitis in an immunocompromised patient who underwent autologous peripheral blood stem cell transplantation.

7

Emerging linezolid-resistant, vancomycin resistant Enterococcus faecium from a patient of a haematological unit in Poland

85%

Krawczyk B. , Samet A. , Bronk M. , Hellmann A. , Kur J.

Polish Journal of Microbiology

|

2004

|

tom 53

|

nr 3

EN

The oxazolidinone antimicrobial, linezolid, has been approved for the treatment of infections caused by various gram-positive bacteria, including vancomycin-resistant enterococci (VRE). This is the first report on isolation of a linezolid resistant, vancomycin-resistant Enterococcus faecium (LRVREF) strain in Poland, from a Haematological Unit patient in the Clinical Hospital in Gdańsk. PCR-RFLP analysis of rDNA and allele-specific PCR of the domain V region of the 23S ribosomal RNA gene demonstrated the presence of the G2576U mutation previously reported to be associated with linezolid resistance. Both assays detected heterozygous in this position.

8

Effect of potential probiotic activity of Enterococcus faecium EE3 strain against Salmonella infection in Japanese quails

85%

Marcinakova M. , Strompfova V. , Boldizarova K. , Laukova A. , Gancarcikova S.

Bulletin of the Veterinary Institute in Puławy

|

2004

|

tom 48

|

nr 4

9

Wymienialnosc sideroforow w obrebie rodzaju Enterococcus

71%

Szarapinska-Kwaszewska J. , Mikucki J.

Medycyna Doświadczalna i Mikrobiologia

|

2000

|

tom 52

|

nr 3

237-245

PL

Zbadano zdolność do wymiany sideroforów między szczepami i gatunkami rodzaju Enterococcus. Większość badanych 62 szczepów należała do gatunków E. faecalis i E. faecium. Stwierdzono, że enterokoki mają zdolność posługiwania się sideroforami syntetyzowanymi przez szczepy własnego i innych gatunków tego rodzaju, choć obserwowano różnice w zachowaniu się poszczególnych szczepów.

EN

The functional interchangeability of siderophores was tested among 62 strais belonging to 12 species of genus Enterococcus. Most investigated strains were from E. faecalis and E. faecium species. The majority of examined enterococcal strains appeared highly resistant to EDDA (ethylene-di-amine-di-ortho-hydroxyphenylacetic acid), therefore the group of sensitive strains involved only 11 used as indicator strains. The determination of interchangeability of siderophores within enterococcal strains was performed using EDDA-agar media into which the indicator strains were included. Test colonies (donor strains) were applied to the surface of the media to determine whether the indicator organisms could obtain the required iron for growth by utilizing chelators from the test colony. Only two strains: E. solitarius DSM 5634 and E. pseudoavium DSM 5632 did not demonstrate the ability to utilize siderophores synthesized by all investigated strains. The other tested indicator strains appeared to be recipients of siderophores from 20-52 donor enterococcal strains. The ability to exchange siderophores in enterococci was found as the feature characterizing individual strains.

10

Lekowrazliwosc szczepow Enterococcus sp. izolowanych z materialu klinicznego oraz od osob zdrowych

71%

Piekarska K. , Lawrynowicz-Paciorek M. , Magdziak A. , Bajera E. , Rudecka A. , Wyrebiak A. , Pietrzyk M. , Kochman M.

Medycyna Doświadczalna i Mikrobiologia

|

2002

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tom 54

|

nr 4

305-315

PL

Określono lekowrażliwość 154 szczepów Enterococcus sp. izolowanych od pacjentów pięciu warszawskich szpitali oraz 33 szczepów izolowanych z próbek kału osób zdrowych. Wszystkie badane szczepy E. faecalis i E. faecium były wrażliwe na antybiotyki glikopeptydowe. Spośród szczepów izolowanych z próbek materiału klinicznego oporność na penicylinę wykazywało 100% szczepów E. faecium i 33,3% - E. faecalis. Oporność na wysokie stężenia aminoglikozydów (HLAR) wykazywało 68,9% szczepów E. faecium i 28,3% E. faecalis. Szczepy enterokoków pochodzące od ludzi zdrowych były wrażliwe na większość antybiotyków użytych do badań.

EN

The aim of this study was to evaluate the drug susceptibility of Enterococcus sp. strains isolated in 2000-2001, from patients of five Warsaw's hospitals (154 strains) and from fecal samples of healthy persons (33 strains). On biochemical reaction profiles species of clinical enterococci were identified as: E. faecalis - 66,2%, E. faecium - 29.2%, E. hirae - 1,9%, E. gallinarum - 1,3%, E. casseliflavus - 0,6% and E. avium - 0,6%. The species of enterococci from stool's samples were identified as: E. faecalis - 28 strains, E. durons - 2 strains and single strains: E. faecium, E. gallinarum and E. casseliflavus. Susceptibility to 20 antibiotics was tested by disc diffusion method. None of these 187 enterococcal strains was vancomycin resistant; 3 strains of E. gallinarum and 1 - E. casseliflavus demonstrated intermediately susceptibility to vancomycin, but they were susceptible to teicoplanin - phenotype Van C. Among clinical strains were resistant to penicillin - 33,3% of E. faecalis and 100% of E. faecium, to ampicillin - over 80% of E. faecium and 1 strain of E. faecalis. None of these strains produced ß-Iactamase. High-level resistance to aminoglicoside was expressed by 48 strains (47,1%) E. faecalis and 36 (80%) E. faecium isolated from clinical specimens. Both – HLR to streptomycin and gentamycin were found in 28.3% of E. faecalis and 68,9% of E. faecium. Among 33 strains isolated from fecal samples of healthy persons - 3 of E. faecalis were resitant to streptomycin and one was resistant to both gentamicin and streptomycin. In general, enterococcal strains isolated from samples of healthy persons were susceptible to the most of used antibiotics. But to rifampicin none of these strains were susceptible. There were about 40% of E. faecalis strains isolated from healthy persons, resistant to tetracyline.

11

Zakazenia fenotypem Van B Enterococcus faecium i E.faecalis u chorych z niedoborem immunologicznym w przebiegu chorob nowotworowych ukladu krwiotworczego

71%

Kedzierska J. , Wegrzyn J. , Skotnicki A. B. , Skop A. , Pawliszyn W.

Medycyna Doświadczalna i Mikrobiologia

|

2003

|

tom 55

|

nr 1

11-24

PL

W pracy przeanalizowano drugą, udokumentowaną w Polsce epidemię VRE wywołaną fenotypem VanB. Uzyskano jeden izolat VREM oporny na teikoplaninę od chorego będącego w trakcie terapii tym antybiotykiem. Potwierdzono zależność pomiędzy wcześniejszym stosowaniem wankomycy- ny, cefalosporyn i imipenemu a częstością występowania zakażeń i kolonizacji VRE. Odnotowano znaczący udział analizowanych szczepów VRES i VREM jako czynników przyczynowych jednorodnych etiologicznie zakażeń uogólnionych w badanej grupie chorych.

EN

In this work, we have analyzed the second outbreak of VRE with the VanB phenotype to be identified in the country. The aim of this study was to characteristics of the types of resistance to glycopeptide antibiotic and to check the resistance patterns of these pathogens. A trial of monitoring the risk factors for colonization or infection with VRE as well as epidemiological investigation were undertaken. Genus identification of the isolates was performed according to the method of Facklam and Collins, and species were identified using the API 20 Strep test. MICS of different antimicrobial agents were determined by the E-test method. The isolates collected during the investigation demonstrated resistance to multiple antimicrobials, which is a common characteristic of VRE. Isolates were found to be uniformly resistant to penicillin, fluoroquinolones, tetracycline and to high concentrations of aminoglycosides. The only drugs with in vitro activity against the isolates were ampicillin (VRES), linezolid (VRES,VREM) and quinupristin-dalfopristin (VREM). Except for a single VREM isolate, they all revealed the typical VanB phenotype with resistance to vancomycin and susceptibility to teicoplanin. One of the VREM isolates turned out to be resistant to teicoplanin, which coincided with the use of this antibiotic in the patient's therapy. Its vanB gene variant differed by a single mutation from that found in other isolates; however, it also lacked a large part of the vanB gene claster, including the regulatory genes vanRв and -Sb, and the vancomycin - inducible promoter Pyb- Our studies have found an association between colonization or infection with VRE and the mean duration of hospital stay, previously administration of glycopeptide, cephalosporins and imipenem. These organisms were a common cause of monoetiological bloodstream infections.

12

Sterowanie procesem pasteryzacji konserw miesnych na podstawie nowego modelu wyznaczania stopni letalnosci

71%

Danyluk B.

Rozprawy Naukowe. Akademia Rolnicza im. Augusta Cieszkowskiego w Poznaniu

|

2007

|

nr 385

1-92

13

Antibiotic resistance genes and virulence factors in Enterococcus faecium and Enterococcus faecalis from diseased farm animals: pigs, cattle and poultry

71%

Seputiene V. , Bogdaite A. , Ruzauskas M. , Suziedeliene E.

Polish Journal of Veterinary Sciences

|

2012

|

tom 15

|

nr 3

EN

Eighty enterococcal isolates (E. faecium, n=38, E. faecalis, n=42) from diseased farm animals (swine, cattle, poultry) in Lithuania have been studied for the prevalence of antibiotic resistance and for resistance and virulence genetic determinants. 86% of E. faecium and 71% of E. faecalis isolates were multidrug resistant (resistant to three or more unrelated antibiotics). Resistance to aminoglycosides, tetracycline and erythromycin was found most frequently in both species (61%, 69%) and was linked to aph(3’)-IIIa, aac(6’)-Ie-aph(2”)-Ia, ant(6)-Ia (aminoglycoside resistance), tetM, tetL (tetracycline resistance), ermA, ermB (erythromycin resistance) gene combinations, which were supplemented with chloramphenicol resistance genes catA7, catA8 (E. faecalis) and catA9 (E. faecium). All E. faecalis isolates harboured genes coding for virulence factors agg, esp, fsr gelE alone or in combinations with the high prevalence of esp gene in isolates from cattle (63%) and pigs (79%). The origin- dependent incidence of agg gene variants prgB and asp1 was observed. The results indicate the existence of a large pool of potentially virulent and multidrug resistant E. faecalis in diseased farm animals posing risk to humans.

14

Genetyczne podobieństwo opornych na wankomycynę szczepów Enterococcus faecium izolowanych z materiału klinicznego

71%

Mlynarczyk A. , Grzybowska W. , Mrowka A. , Tyski S. , Mlynarczyk G.

Medycyna Doświadczalna i Mikrobiologia

|

2010

|

tom 62

|

nr 4

PL

Badano występowanie genów vanA, vanB, vanD, vanE i vanG metodą PCR u wankomycyno-opornych szczepów Enterococcus faecium izolowanych z materiału klinicznego pochodzącego z wybranych oddziałów Szpitala Dzieciątka Jezus w Warszawie oraz ustalono podobieństwo szczepów metodą analizy polimorfizmu restrykcyjnych fragmentów genomowego DNA za pomocą (RFLP) - PFGE

EN

Twenty vancomycin resistant E.faecium strains (VRE) isolated from patients of three different hospital wards in 2005 - 2008 were examined. The strains originated from patients of intensive therapy, urological and internistic wards. The chosen wards differ significantly in their specificity. In all cases the presence of o vanA and lack of vanB, vanD, vanE and vanG genes and were found. Strains were compared by using RFLP-PFGE, the reference method for molecular typing of VRE. One group including fourteen strains showing similarity higher than 79.5% was distinguished. This group was divided into subgroups. The greatest similarity was found among strains from patients of intensive therapy ward. Two subgroups of strains showing similarity more than 93.3%, of four strains each were identified. The similarity between these two subgroups was 79.5%. Most strains from other two wards showed less than 79.5% similarity and they could be recognised as not related. Only one strain from internal ward and two strains from urologie ward were similar in 82.1 - 86.4% to one of subgroups of strains originated from intensive therapy.

15

Enterokoki - wlasciwosci funkcjonalne i wykorzystanie

71%

Gajewska J. , Rekiel A. , Pawlicka E.

Przegląd Hodowlany

|

2008

|

tom 76

|

nr 08

17-19

16

Antimicrobial resistance and virulence factor gene profiles of Enterococcus spp. isolated from giant panda oral cavities

71%

Zhong R. , Zhou Z. , Liu H. , Zhong Z. , Peng G.

Journal of Veterinary Research

|

2021

|

tom 65

|

nr 2

17

Occurrence of antibiotic resistant enterococci in clinical specimens from a pediatric hospital

71%

Rudy M. , Zientara M. , Bek T. , Martirosian G.

Polish Journal of Microbiology

|

2005

|

tom 54

|

nr 1

EN

Enterococcal infection has become a major clinical problem and E. faecalis and E. faecium are the most frequently isolated species. However, the isolation of other species (E. casseliflavus, E. gallinarwn, E. durans) from clinical materials was reported recently. The aim of this study was to evaluate drug resistance of 97 species of enterococci isolated from clinical specimens of Upper Silesian Health Center of Child and Mother in Katowice. Each strain was tested for susceptibility to vancomycin, teicoplanin, aminoglycosides (gentamycin and streptomycin) and synercid (quinupristine/ dalfopristine) by the E-test method. Fifty three percent of studied enterococci demonstrated high level aminoglycoside resistance (HLAR) (MIC > 1024 μg/ml). Sixty three strains of E. faecalis were sensitive to vancomycin (MIC 1-4 μg/ml), but 5 strains demonstrated low sensitivity (3 strains with MIC = 6 μg/ml and 2 strains with MIC = 24 μg/ml). All studied enterococci were sensitive to teicoplanin (MIC < 2 μg/ml). A high percentage of E. faecium (70%) resistant to synercid was demonstrated (MIC = 2-24 μg/ml). Infection control and monitoring of antibiotic sensitivity among isolated hospital strains may prevent the transmission of resistant strains in a pediatric hospital.

18

Prevalence of high level aminoglycoside and vancomycin resistance among enterococci in Turkey

71%

Cirak M. Y. , Sultan N.

Acta Microbiologica Polonica

|

1998

|

tom 47

|

nr 3

19

Use of bacteriocin preparation with anti-microbial activity in Saint-Paulin cheese

71%

Laukova A. , Vlaemynck G.

Bulletin of the Veterinary Institute in Puławy

|

2003

|

tom 47

|

nr 2

20

New plant glycoprotein against methicillin resistant staphylococci and enterococci

71%

Fik E. , Gozdzicka-Jozefiak A. , Haertle T. , Mirska I. , Kedzia W.

Acta Microbiologica Polonica

|

1997

|

tom 46

|

nr 3