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Mikotoksyny wytwarzane przez izolaty Bipolaris sorokiniana i Drechslera dictyoides w warunkach in vitro

100%

Horodecka M.

Genetica Polonica

1994

tom 35A

95-100

Tolerancja na toksyny a stopien porazenia lisci wybranych genotypow kostrzewy lakowej przez Drechslera dictyoides [Drechsl.] Shoem.

100%

Plazek A.

Genetica Polonica

1994

tom 35A

105-109

The influence of Bipolaris sorokiniana and Drechslera dictyoides metabolites on cell membrane permeability of Festuca pratensis callus

100%

Plazek A. , Filek M.

Acta Physiologiae Plantarum

1998

tom 20

nr 3

The aim of this study was the evaluation of membrane permeability of callus cells of several Polish meadow fescue cultivars, which were treated with toxins of two leaf spot pathogens Bipolaris sorokiniana and Drechslera dictyoides. Fungus metabolites were obtained by the method described by Lepoivre et al. (1986). Calli of cultivars ‘Skrzeszowicka’, ‘Skawa’, ‘Westa’, POB 282, POB 383, KOA 186 have been selected on medium with metabolites for two weeks. Next the conductivity test of electrolyte leakage and of total ion contents in the examined tissue was done. On the base of this data the membrane permeability coefficients for each cultivar were calculated. Toxins of B. sorokiniana damaged the cell membranes more strongly than metabolites of D. dictyoides. The significant differences of several objects sensitivity to the influence of B. sorokiniana metabolites were stated. These differences were not observed in the case of the influence of D. dictyoides metabolites on the examined tissue.

Relationship between soluble carbohydrate level and tolerance of meadow fescue callus to Bipolaris sorokiniana [Sacc.] Shoem. and Drechslera dictyoides [Drechsl.] Shoem. metabolites

100%

Plazek A.

Acta Physiologiae Plantarum

1998

tom 20

nr 4

The aim of the presented work was the search for the relationship between the level of soluble carbohydrates in callus tissues of eight meadow fescue (Festuca pratensis Huds.) cultivars and their growth ability on media containing Bipolaris sorokiniana and Drechslera dictyoides metabolites. Calli were induced from mature grains using the method previously described (Płażek 1994). Callus obtained from single caryopsis was cut into three pieces which were weighted and put on the media with or without pathogen metabolites. Tissue selection was performed by means of “double-layer culture” technique (Lepoivre et al. 1986). After two-week culture in the darkness at temp. of 25°C the calli were weighted again. The sugar level in tissue was measured by means of colorimetric method of Klein & Weissman according to Snell (1961). Fresh mass decrease of calli developing on the media with fungus metabolites was observed by all studied object. The tolerance of calli of the tested cultivars to metabolites of both pathogens was significantly different. However, significant similarity between the tolerance of calli of particular varietes to both fungi was noted. The soluble carbohydrate contents in control tissue of all studied cultivars were similar and their values ranged between 2.4 and 3 % of fresh mass. B. sorokiniana metabolites caused a significant decrease of the sugar content in calli, while D. dictyoides metabolites did not decrease the sugar level.

The possibility of tissue culture method used in grass resistance breeding: sugar influence on mycelium growth and metabolite toxicity of Bipolaris sorokiniana and Drechslera dictyoides

80%

Plazek A.

Acta Physiologiae Plantarum

1997

tom 19

nr 2

Mikoflora lisci Festuca pratensis Huds

71%

Kutrzeba M.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

1993

tom 188

33-41

Ocena odpornosci kalusa kostrzewy lakowej [ Festuca pratensis Huds.] na metabolity grzybow z rodzaju Drechslera sp.

71%

Plazek A.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

1993

tom 188

43-50