Wyniki wyszukiwania - Biblioteka Nauki

1

Effect of the vapour phase on the TLC separation of tropane alkaloids

100%

Gadzikowska M. , Jóźwiak G. W. , Waksmundzka-Hajnos M.

Acta Chromatographica

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2010

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tom Vol. 22, no. 4

515--525

EN

The effect of conditioning the silica layer by mobile phase vapour or ammonia vapour on the retention of alkaloids eluted with non-aqueous mobile phases was examined. The effect of vapour phase composition on system efficiency, peak symmetry, and separation selectivity were also investigated. The most effective and selective systems were used for separation of the alkaloid fraction of Datura innoxia.

2

Influence of sample preparation methods on the quantitation of selected tropane alkaloids from herb of Datura innoxia mill. by HPTLC

86%

Oniszczuk A. , Waksmundzka-Hajnos M. , Gadzikowska M. , Podgórski R. , Oniszczuk T.

Acta Chromatographica

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2013

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tom Vol. 25, no. 3

545--554

EN

The aim of our paper was the examination of various procedures of extraction and purification methods on the yield of atropine and scopolamine in Datura innoxia extracts determined by high-performance thin-layer chromatography (HPTLC). In our experiments, the following extraction methods were used: percolation, ultrasound-assisted extraction (USAE) at various temperatures, and microwave-assisted extraction (MASE). The highest extraction yields of atropine and scopolamine were obtained by ultrasound-assisted extraction method and aqueous acetic acid or aqueous tartaric acid used as extractants. Crude extracts of D. innoxia herb were purified by use of various methods: solid-phase extraction-strong cation exchanger (SPE-SCX), SPE-C18, and liquid-liquid extraction (LLE). The best results of purification with satisfactory %RSD values were obtained by liquid-liquid extraction by use of dichloromethane from aqueous crude alkaloid extract alkalized to pH 12. HPTLC was performed on pre-coated plates Kieselgel 60 F254 in horizontal DS chambers by use of mobile phase: methanol-acetone-aqueous ammonia (5:4.5:0.5). The alkaloids’ content was determined in extracts by use of calibration curve method for atropine and scopolamine. Calibration curves were linear in range of 3.0–30 μg for scopolamine (R = 0.9980) and 2.6–26 μg for atropine (R = 0.9923). Limits of detection (LOD) and limits of quantification (LOQ) were 1.12 μg and 3.42 μg per spot for scopolamine and 0.89 μg and 2.82 μg per spot for atropine.

3

Dynamika wzrostu i plonowanie bielunia indianskiego [Datura innoxia Mill.] na tle warunkow pogodowych

86%

Pakula I. , Buczkowska H. , Bednarek H.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EEE: Horticultura

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2006

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tom 16

93-100

4

Rosliny narkotyczne i halucynogenne w zatruciach mlodziezy. Cz.III

58%

Lamer-Zarawska E.

Wiadomości Zielarskie

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2001

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tom 43

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nr 12

14-16

5

Wpływ sposobu uprawy na plonowanie bielunia indiańskiego (Datura innoxia Mill.) oraz zawartość alkaloidów w zielu

58%

Pakula I. , Buczkowska H.

Folia Horticulturae. Suplement

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2006

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nr 2

6

A strategy for overaccumulation of scopolamine in Datura innoxia hairy root cultures

58%

Dechaux C. , Boitel-Conti M.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

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nr 1

EN

Hairy root culture plays an important role in investigation of alkaloid production in culture in vitro. The complexity of scientific work and the production capacity limitations associated with whole plant systems and unorganized cells can be overcome using culture of hairy roots. This paper presents an example of a strategy to produce secondary metabolites from plants, in which we investigated the alkaloid pathway, modified gene expression, and cultivated and optimized hairy root growth in a bioreactor. Datura innoxia whole plant was transformed by Agrobacterium rhizogenes, and the obtained hairy roots were studied for their tropane alkaloid production. Optimization of medium led to an increase of scopolamine production in hairy root culture of Datura innoxia by increasing the produced biomass, but genetic engineering seems to be the best way to increase the accumulation of scopolamine. These root cultures were not able to stably overaccumulate scopolamine. The involvement of putative enzymatic regulation of the tropane alkaloid pathway is discussed.

7

Yield of a herb [Datura innoxia Mill.] and content of alkaloids depending on development stage of harvested plants

58%

Buczkowska H. , Pakula I.

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nr 3

EN

The experiments were carried out in 2004-2006 in the Department of Vegetable and Medicinal Plants, Agricultural University in Lublin. The aim of presented work was to evaluate a development stage in which should the Datura innoxia plants be harvested to obtain herb with high content of alkaloids per area unit. Plants were harvested: 1 - in a stage of vegetative growth, 2 - in the beginning of flowering, 3 - in the beginning of fructification. The effects of weather conditions on air-dry herb yield (17.0-23.2 kg-100 m~2) and content of tropane alkaloids (0.166-0.224%) was stated. Significantly lower mean herb yield was obtained from plants harvested in vegetative growth stage. Significant relationship between tropane alkaloids content and development stage of plants was not stated. Quantity of tropane alkaloids obtained in cultivation of Datura innoxia (22.32-55.57 gl 00 nr2) depended more on high value of air-dry herb yield per area unit (12.9-28.5 kg-100 m2) than on content of these active substances in material (0.150-0.236%).

PL

Badania przeprowadzono w latach 2004-2006 w Katedrze Warzywnictwa i Roślin Leczniczych AR w Lublinie. Celem niniejszej pracy było stwierdzenie, w jakiej fazie rozwojowej należy zbierać rośliny bielunia indiańskiego, by uzyskać ziele o dużej ilości alkaloidów z jednostki powierzchni. Zbiory przeprowadzono: 1 - w fazie wzrostu wegetatywnego, 2 - na początku kwitnienia, 3 - na początku owocowania. Stwierdzono wpływ warunków pogodowych na plon powietrznie suchego ziela (17,0-23,2 kg-100 m"2) oraz na zawartość alkaloidów tropanowych (0,166-0,224%) w tym zielu. Istotnie mniejszy średni plon ziela otrzymano z roślin zbieranych w okresie wzrostu wegetatywnego. Nie stwierdzono statystycznie istotnej zależności zawartości alkaloidów tropanowych od fazy rozwojowej roślin. Ilość alkaloidów tropanowych (22,32-55,57 g • 100 m'2) uzyskanych z uprawy bielunia indiańskiego zależała w dużym stopniu od wielkości plonu powietrznie suchego ziela zebranego z jednostki powierzchni (12,9-28,5 kg-100 nr2), zaś w następnej kolejności od zawartości tych substancji czynnych w surowcu (0,150-0,236%).

8

Nasiona bielunia dziedzierzawy przyczyna niebezpiecznych zatruc

51%

Szypula W.

Wiadomości Zielarskie

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2001

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tom 43

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nr 10

14-15

9

Androgenic development: a fascinating embryo formation process

44%

Goralski G. , Matthys-Rochon E. , Vergne P. , Przywara L.

Acta Biologica Cracoviensia. Series Botanica

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1999

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tom 41

EN

The subject of this review is androgenesis as a process of embryo development from microspores and pollen. A number of factors govern androgenesis, but genotype, the stage of microspore development and in vitro culture conditions are the most important. We summarize some recent work employing a variety of experimental approaches to study this fascinating phenomenon of embryo formation.