Wyniki wyszukiwania - Biblioteka Nauki

1

A novel repetitive DNA sequence in lemon [Citrus limon [L.] Burm.] and related species

100%

De Felice B. , Wilson R. R. , Ciarmiello L. , Conicella C.

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nr 3

2

Analysis of unstable DNA sequence in FRM1 gene in Polish families with fragile X syndrome

88%

Milewski M. , Zygulska M. , Bal J. , Deelen W. H. , Obersztyn E. , Bocian E. , Halley D. J. J. , Horst J. , Mazurczak T.

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tom 43

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nr 2

3

Molecular biogeography of red deer Cervus elaphus from eastern Europe: insights from mitochondrial DNA sequences

75%

Niedzialkowska M. , Jedrzejewska B. , Honnen A.-C. , Otto T. , Sidorovich V. E. , Perzanowski K. , Skog A. , Hartl G. B. , Borowik T. , Bunevich A. N. , Lang J. , Zachos F. E.

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nr 1

EN

European red deer are known to show a conspicuous phylogeographic pattern with three distinct mtDNA lineages (western, eastern and North-African/Sardinian). The western lineage, believed to be indicative of a southwestern glacial refuge in Iberia and southern France, nowadays covers large areas of the continent including the British Isles, Scandinavia and parts of central Europe, while the eastern lineage is primarily found in southeast-central Europe, the Carpathians and the Balkans. However, large parts of central Europe and the whole northeast of the continent were not covered by previous analyses. To close this gap, we produced mtDNA control region sequences from more than 500 red deer from Denmark, Germany, Poland, Lithuania, Belarus, Ukraine and western Russia and combined our data with sequences available from earlier studies to an overall sample size of almost 1,100. Our results show that the western lineage extends far into the European east and is prominent in all eastern countries except for the Polish Carpathians, Ukraine and Russia where only eastern haplotypes occurred. While the latter may actually reflect the natural northward expansion of the eastern lineage after the last ice age, the present distribution of the western lineage in eastern Europe may in large parts be artificial and a result of translocations and reintroduction of red deer into areas where the species became extinct in historical times.

4

Evaluation of methods for Erwinia amylovora detection

75%

Kaluzna M. , Pulawska J. , Mikicinski A.

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tom 21

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nr 2

5

Morphological and molecular characterisation of Myxidium kudoi Meglitsch, 1937 from the blue catfish Ictalurus furcatus, Valenciennes in Oklahoma, USA

75%

Woodyard E. T. , Rosser T. G. , McAllister C. T.

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tom 65

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nr 2

6

Characteristics and a comparison of three classes of microsatellite-based markers and their application in plants

75%

Rakoczy-Trojanowska M. , Bolibok H.

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2004

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tom 09

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nr 2

EN

Microsatellites (SSR - simple sequence repeats, STR - short tandem repeats, SSLP - simple sequence length polymorphism, VNTR - variable number of tandem repeats) are the class of repetitive DNA sequences present in all living organisms. Particular characterstics of microsatellites, such as their presence in the genomes of all living organisms, high level of allelic variation, co-dominant mode of inheritance and potential for automated analysis make them an excellent tool for a number of approaches like genotyping, mapping and positional clonig of genes. The three most popular types of markers containing microsatellite sequences that are presently used are: (1) SSR (simple sequence repeats), generated by amplifying in a PCR reaction with the use of primers complementary to flanking regions; (2) ISSR (inter-simple sequence repeats), based on the amplification of regions between inversely oriented closely spaced microsatellites; and (3) SAMPL (selective amplification of microsatellite polymorphic loci), which utilises AFLP (amplified fragment-length polymorphism) methodology, with one exception - for the second amplification, one of the starters is complementary to the microsatellite sequence. The usefulness of the three above-mentioned markers for numerous purposes has been well documented for plants.

7

Recent practice in biotechnology patenting at the EPO

75%

Heimann-Pohl B.

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nr 3

8

Diminished expression of the type II receptor for TGFbeta [TGFbetaRII] in T lymphocytes from patients with Sezary syndrome is not due to mutations in the receptor's poly-A tract: limitations of the standard RT-PCR in cDNA sequence analysis of homopolymeric base stretches

75%

Zhang Q. , Capocasale R. J. , Fox F. E. , Bedian V. , Vonderheid E. C. , Rook A. , Moore J. S. , Nowell P. C. , Haines D. S. , Wasik M. A.

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nr 6

9

Regulation of lncRNA expression

63%

Wu Z. , Liu X. , Liu L. , Deng H. , Zhang J. , Xu Q. , Cen B. , Ji A.

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nr 4

EN

Long non-coding RNAs (lncRNAs) are series of transcripts with important biological functions. Various diseases have been associated with aberrant expression of lncRNAs and the related dysregulation of mRNAs. In this review, we highlight the mechanisms of dynamic lncRNA expression. The chromatin state contributes to the low and specific expression of lncRNAs. The transcription of non-coding RNA genes is regulated by many core transcription factors applied to protein-coding genes. However, specific DNA sequences may allow their unsynchronized transcription with their location-associated mRNAs. Additionally, there are multiple mechanisms involved in the post-transcriptional regulation of lncRNAs. Among these, microRNAs might have indispensible regulatory effects on lncRNAs, based on recent discoveries.

10

Annotating a non-model plant genome – a study on the narrow-leafed lupin

63%

Zielezinski A. , Potarzycki P. , Ksiazkiewicz M. , Karlowski W. M.

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nr 3

11

Transcriptional activity of a complex of wheat RNA polymerase II and nonhistone chromosomal protein (NHCP)

63%

Dullin P. , Galbas M. , Chodynicka-Kordus G. , Fabisz-Kijowska A. , Walerych W.

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nr 3-4

12

PCR-based molecular markers for identification of taxa from the Calypogeia fissa complex (Jungermanniopsida, Calypogeiaceae)

63%

Buczkowska K. , Gonera P. , Hornik B.

Biodiversity: Research and Conservation

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2012

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tom 28

13

New records of Dactylobiotus parthenogeneticus Bertolani, 1982 provide insight into its genetic variability and geographic distribution

63%

Pogwizd J. , Stec D.

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nr 2

14

Allopolyploid character and the origin of organells in Pellia borealis based on the nucleotide sequences data

63%

Szweykowska-Kulinska Z. , Pacak A. , Fiedorow P.

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nr 2

15

Polymorphism of bovine microsatellite DNA sequences in the lowland European bison

63%

Gralak B. , Krasinska M. , Niemczewski C. , Krasinski Z. A. , Zurkowski M.

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nr 4

EN

Investigations of genetic polymorphism of microsatellite DNA sequences were conducted in 22 individuals of the European bisonBison bonasus (Linneaus, 1758) from Białowieża Primeval Forest. For this purpose 27 cattle microsatellite primer pairs were used. Among the 27 microsatellite markers examined, an amplification product was obtained for 21 loci. This rendered it possible to identify total of 40 alleles in the bison population tested. In addition, eight loci were proved to be monomorphic. A majority of the 40 alleles identified was identical with the alleles identified at the corresponding loci in cattle. Only two alleles seem to be specific for the European bison. The value of heterozygosity for the examined loci in bison population from Białowieża was low and ranged from 0.13 to 0.53. Hence, the polymorphism information content was low as well. Based on our results the microsatellite DNA markers identified in cattle may be used to analyse the genetic structure of the population of European bison.

16

Characterization of DNA sequences localized 11 to 17.5 kb upstream of the chicken embryonic pi-globin gene

63%

Pietrowska M. , Rusin M. , Widlak P. , Razin S. V. , Rzeszowska-Wolny J.

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nr 3

EN

We have analyzed the DNA fragment localized about 11 to 17.5 kb upstream of the chicken α-globin gene domain (the fragment was designed as α-0). The nucleotide sequence of its 3.3 kb-long 5' part was established and interactions with nuclear matrix proteins were studied. The DNA region localized about 16 kb upstream of the embryonic π-globin gene showed high affinity to nuclear matrices in vitro. Two palindromes and a cluster of inverted repeats were co-localized in the same region. The whole 6.6 kb α-0 fragment decreased the activity of CAT reporter gene when transfected into chicken erythroblastoid cells.

17

The role of mammalian DNA methyltransferases in the regulation of gene expression

63%

Turek-Plewa J. , Jagodzinski P. P.

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nr 4

18

Comparison of arbitrary primer [AP] PCR and pulsed-field gel electrophoresis methods for genotypic differentiation of Escherichia coli O157 strains

63%

Weiner M. , Osek J.

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nr 2

19

Identification and characterization of the Trichoderma harzianum gene encoding alpha-1,3-glucanase involved in streptococcal mutan degradation

51%

Wiater A. , Janczarek M. , Pleszczynska M. , Szczodrak J.

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nr 4

EN

α-1,3-Glucanases (mutanases) are currently of great interest due to their potential use in the field of dental care. These enzymes have been reported in several bacteria, yeasts and fungi, but up to now, characterization of this family of proteins has been relatively poor. In this study, we identify and characterize a mutanase gene from Trichoderma harzianum CCM F-340. Sequence analysis, on the nucleotide and amino acid levels reveals that this α-1,3-glucanase is highly homologous to α-1,3-glucanases from T. harzianum isolate CBS 243.71 and T. asperellum CECT 20539. T. harzianum CCM F-340 mutanase is a 634-aa residue protein with a calculated molecular mass of 67.65 kDa, composed of two distinct, highly conserved domains (a long N-terminal catalytic domain and a short C-terminal polysaccharide-binding domain) separated by a less conserved Pro-Ser-Thr-rich linker region. The mutanase gene was expressed in an E. coli BL21(DE3) host, under the transcriptional control of T7 promoter. The purified enzyme migrated as a band of about 68 kDa after SDS-polyacrylamide gel electrophoresis, which coincided with the predicted size based on the amino acid sequence. Our data indicate that this enzyme is highly conserved in Trichoderma and can be produced in active form in such heterologous expression system.

20

The potential role of Kruppel-like zinc-finger protein glis3 in genetic diseases and cancers

51%

Chou C.-K. , Tang C.-J. , Chou H.-L. , Liu C.-Y. , Ng M.-C. , Chang Y.-T. , Yuan S.-S. F. , Tsai E.-M. , Chiu C.-C.

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nr 5