Wyniki wyszukiwania - Biblioteka Nauki

1

Potato genetics based on DNA markers

100%

Gebhardt C. , Leister D. , Ballvora A. , Meksem K. , Niewochner J. , Schafer-Pregl R. , Salamini F.

Journal of Applied Genetics

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1996

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tom 37A

2

Genetic relationships between some of Malva species as determined with ISSR and ISJ markers

88%

Celka Z. , Szczecinska M. , Sawicki J.

Biodiversity: Research and Conservation

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2010

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tom 19

3

Phylogeographic investigation of Hordeum murinum L. in Europe based on DNA markers

88%

Bieniek W.

Acta Biologica Cracoviensia. Series Botanica

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2018

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tom 60

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nr 1

4

Genetic similarity of Pinus sylvestris populations on the base of DNA markers

88%

Polok K. , Urbaniak L. , Korzekwa K. , Androsiuk P. , Ciaglo S. , Kubiak K. , Zielinski R.

Seria Biologiczna. Uniwersytet im. Adama Mickiewicza w Poznaniu

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2005

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tom 72

5

Application of a complementary set of 10 microsatellite DNA markers for parentage verification in Polish Red cattle

75%

Radko A.

Annals of Animal Science

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2010

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tom 10

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nr 1

9-15

6

Molecular identification of blast resistance genes in rice genotypes using gene-specific markers

75%

Al-Daej M. I. , Ismail M. , Rezk A. A. , El-Malky M. M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2019

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tom 100

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nr 3

7

Comparison of two Pellia endiviifolia sibling species on the base of RAPD and ISJ markers

75%

Polok K.

Biological Bulletin of Poznań

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2001

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tom 38

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nr 2

8

Gene and genome mapping in cereals

63%

Borner A.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2A

EN

The trait ﬂowering time regulated by genes determining alisation and photoperiod sensitivity was used as an example for presenting data on comparative major gene and QTL mapping within the Triticeae. The major genes are shown to be members of homoeologous series. Furthermore it was demonstrated that in genome regions carrying major genes also QTLs for the same traits were detected.

9

Bi-paternal litter in Finn raccoon [Nyctereutes procyonoides Gray 1834] detected by polymorphic DNA markers

63%

Slaska B. , Jezewska G.

Folia Biologica

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2008

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tom 56

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nr 3-4

193-195

10

RAPD analysis of genetic structure in four natural populations of Taxus baccata from southern Poland

63%

Zarek M.

Acta Biologica Cracoviensia. Series Botanica

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2009

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tom 51

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nr 2

67-75

EN

This work assessed genetic diversity and genetic structure using random amplified polymorphic DNA (RAPD) variation in 120 individuals of four natural populations of Taxus baccata growing in southern Poland (3 in mountains and one in lowland) to obtain basic information on this natural resource. With 9 primers, 185 highly reproducible and clear RAPD bands were obtained. Genetic diversity within populations was relatively high, with percentages of polymorphic bands ranging from 48.65% to 77.30%, averaging 69.59% (Shannon index 0.311). Global AMOVA showed that genetic variation between populations accounted for 26% of total variation, with the remainder (74%) occurring within population. Pairwise ΦPT values were not correlated with geographic distance. Two groups of populations were distinguished by ANOVA and principal coordinate analysis (PCO) based on a Euclidean metric: those growing in mountains (Nowa Wieś, Cisowa Gora, Zadni Gaj), with higher internal diversity, and those growing in lowlands (Liswarta), with lower internal diversity. The results are typical for an outcrossing, wind-pollinated and long-lived woody species

11

Application of spermatozoa from neo-males (XX) to induce androgenetic development in rainbow trout (Oncorhynchus mykiss)

63%

Michalik O. , Kowalski R. , Ziomek E. , Hliwa P. , Mieszkowska A. , Rozynski R. , Ocalewicz K.

Folia Biologica

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2017

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tom 65

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nr 2

12

New DNA markers for discrimination between closely-related species and for the reconstruction of historical events; an example using liverworts

63%

Szweykowska-Kulinska Z. , Pacak A. , Jankowiak K.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2A

EN

A survey of fully-sequenced chloroplast genomes revealed that in land plants there are six tRNA genes that have introns. Moreover, the length of a particular tRNA gene intron remains relatively stable across species. However, in algae, the presence of chloroplast tRNA genes containing introns is exceptional. A survey of mitochondrial plant genomes revealed intron-containing tRNA genes are rather rare features, with the exception of tRNASerGCU genes in liverworts and peat-mosses. We isolated and sequenced one mitochondrial and three chloroplast intron-containing tRNA genes and a fragment of the mitochondrial coxIII gene containing the first intron from the following liverwort species: Pellia borealis, Pellia epiphylla-species N, Pellia epiphylla-species S and Porella baueri, Porella cordaeana, Porella platyphylla. We showed that, as in the case of higher plants, the rate of nucleotide substitution is lower in the mitochondrial genome than in the chloroplast genome. Moreover, the comparison of intron nucleotide sequences enabled us to show that in the case of one allopolyploid species, Pellia borealis, organelles were transmitted from one parent species, Pellia epiphylla-species N. In the case of another allopolyploid species, Porella baueri, organelles were also inherited from one parent species, Porella cordaeana. Therefore, organellar inheritance in liverworts seems to be uniparental. It remains clear that analysis of carefully chosen chloroplast and mitochondrial DNA sequences allowed us to reconstruct historical events.

13

A new diagnostic SSR marker for selection of the Rym4-Rym5 locus in barley breeding

63%

Tyrka M. , Perovic D. , Wardynska A. , Ordon F.

Journal of Applied Genetics

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2008

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tom 49

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nr 2

127-134

EN

Genomic sequence AY661558, representing a part of the ВАС contig of the Rym4/Rym5 locus conferring resistance to the barley yellow mosaic virus complex (BaMMV/BaYMV), was exploited in order to develop SSR markers for practical barley breeding. Out of 57 SSR motifs found within this sequence, primers were designed and tested for the 5 SSRs with the highest repeat length. The polymorphic SSR marker QLB1 со-segregated with rym4 and rym5 phenotypes in respective high-resolution mapping populations developed for the construction of the original ВАС contig. The primers targeted 2 sites located 756 bp and 5173 bp downstream of the translation initiation factor 4E (Hv-eIF4E). Physical linkage of the QLB1 marker to the Rym4/Rym5 locus was confirmed experimentally on Morex ВАС 519J14, a seed ВAC of Hv-eIF4E, and ВAC 801A11, which is located proximally to Hv-eIF4E. QLB1 revealed 7 alleles in a set of 100 winter barley lines and cultivars. Five alleles were found within 673 advanced breeding lines derived from applied Polish winter barley breeding programmes, which corresponds to a PIC value of 0.684. No recombinants between Rym4/5 and QLB1 were detected, suggesting that QLB1 can be used efficiently in marker-assisted selection of the Hv-eIF4E-mediated bymovirus resistance.

14

Allelic variation at the VRN-1 locus of Polish cultivars of common wheat (Triticum aestivum L.)

63%

Nowak M. , Kowalczyk K.

Acta Biologica Cracoviensia. Series Botanica

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2010

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tom 52

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nr 2

EN

At a molecular level, the length of the vernalization period of common wheat (Triticum aestivum L.) is determined mainly by three loci: VRN-1, VRN-2 and VRN-3. In hexaploid wheat, the Vrn-A1, Vrn-B1 and Vrn-D1 genes are dominant for spring growth habit and epistatic to the alleles for winter growth habit. We used DNA markers to determine the VRN-1 genotypes of 43 common wheat cultivars from the Polish register. All of the 30 examined winter wheat cultivars carried the recessive vrn-A1 allele, and all of the 13 analyzed spring cultivars carried the dominant Vrn-A1a allele. Moreover, 13 winter and 11 spring cultivars carried the dominant Vrn-B1 allele. These results confirmed that the hexaploid wheat growth habit is determined mainly by the VRN-A1 locus.

15

Evaluation of anthropogenic pollution in river water based on the genetic diversity of Aeromonas Hydrophila

63%

Korzekwa K. , Gołaś I. , Harnisz M.

Archives of Environmental Protection

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2012

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tom Vol. 38, no. 3

41-50

EN

Aeromonas hydrophila is a valuable indicator of the quality of water polluted by sewage and pathogens that pose a risk for humans and cold-blooded animals, including fi sh. The main aim of this research was to evaluate anthropogenic pollution of river water based on genetic diversity of 82 A. hydrophila strains by means of RAPD, semi-random AP-PCR (ISJ) and the rep-BOX conservative repeats test. Genetic diversity of A. hydrophila was HT = 0.28 (SD = 0.02) for all DNA markers (RAPD, semi random and rep-BOX). None of the analyzed electrophoretic patterns was identical, implying that there were many sources of strain transmission. The presence of genes for aerolysin (aerA), hemolysin (ahh1) and the cytotoxic enzyme complex (AHCYTOGEN) was verifi ed for all tested strains, and drug resistance patterns for tetracycline, enrofl oxacin and erythromycin were determined. The most diverse A. hydrophila strains isolated from river water were susceptible to enrofl oxacine (HS = 0.27), whereas less diverse strains were susceptible to erythromycin (HS = 0.24). The presence of the multidrug resistance marker (ISJ4-25; 1100 bp locus) in the examined strains (resistant to three analyzed drugs) indicates that intensive fi sh cultivation affects the microbiological quality of river water.

PL

Aeromonas hydrophila jest cennym wskaźnikiem jakości wody w przypadku zanieczyszczeń ściekami oraz mikroorganizmami względnie patogennymi dla człowieka i zwierząt zimnokrwistych, w tym ryb. Celem niniejszych badań była ocena zanieczyszczenia antropogenicznego na podstawie zróżnicowania genetycznego 82 szczepów A. hydrophila poprzez analizy RAPD, pół-przypadkowo amplifi kowanej klasy AP-PCR (ISJ) i konserwatywnego powtórzenia rep-BOX. Zróżnicowanie genetyczne A. hydrophila wyniosło HT = 0,28 (SD = 0,02) dla wszystkich markerów DNA (RAPD, pół-przypadkowe i rep-BOX). Wszystkie szczepy dla wszystkich markerów ujawniły indywidualny wzór elektroforetyczny, nie ujawniono jednego źródła rozprzestrzeniania się szczepów. U szczepów potwierdzono obecność genów aerolizyny (aerA), hemolizyny (ahh1) i kompleksu enzymów cytotoksycznych (AHCYTOGEN), jak również określono wzorzec oporności na tetracyklinę, enrofl oksacynę i erytromycynę. Najbardziej zróżnicowane okazały się szczepy A. hydrophila wrażliwe na enrofl oksacynę (HS = 0,27) a najmniej zróżnicowane były szczepy wrażliwe na erytromycynę (HS = 0,24). Wyselekcjonowany marker wielolekooporności (locus ISJ4-25, 1100 pz) obecny u szczepów (opornych na 3 rozpatrywane leki) świadczy o wpływie intensywnej hodowli ryb na jakość mikrobiologiczną wody rzecznej.

16

The preliminary stage of SCAR markers development for Ranunculus subgen. Batrachium

63%

Jopek M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

17

Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo

63%

Wachowiak W. , Baczkiewicz A. , Celinski K. , Prus-Glowacki W.

Dendrobiology

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2004

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tom 51

67-72

EN

Four cpDNA regions were analyzed with the use of PCR-RFLP technique and nucleotide sequences of two mtDNA regions were characterized in order to find P. sylvestris and P. mugo species specific markers useful for studies of the species hybridization. The difference in the restriction fragment patterns of trnV-rbcL region after digestion with MvaI endonuclease was detected. The analyses of the species representatives from various geographic regions revealed that the observed polymorphism is species specific. No differences have been disclosed in the analyzed trnS-trnT, trnK1-trnK2, trnC-trnD cpDNA regions. The P. sylvestris and P.mugo mtDNA sequences of orf25 and coxI regions proved to be identical.

18

Application of DNA markers against illegal logging as a new tool for the Forest Guard Service

63%

Nowakowska J. A.

Folia Forestalia Polonica. Series A . Forestry

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2011

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tom 53

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nr 2

EN

DNA markers are currently the most precise tool for forest tree species identification and can be used for comparative analyses of plant material. Molecular diagnosis of evidence and reference material is based on comparing the structure of DNA markers duplicated in the PCR reaction and estimation of the DNA profiles obtained in studied wood samples. For this purpose, the microsatellite DNA markers are the most suitable tool because of their high polymorphism and accurate detection of structural changes in the genome. The analysis of tree stump DNA profiles let avoid timely collection of data such as tree age, diameter, height and thickness, although such a piece of information may advantageous in wood identification process. For each examined tree species, i.e. Pinus sylvestris L., Picea abies (L.) Karst., Quercus robur L. and Q. petraea (Matt.) Liebl., Fagus sylvatica L., Betula pendula L., and Alnus glutinosa L., wood identification was possible via the DNA profiles established on a basis of minimum 4 microsatellite nuclear DNA loci, and at least one cytoplasmatic (mitochondrial or chloroplast) DNA marker. Determination of the DNA profiles provided fast and reliable comparison of genetic similarity between material of evidence (wood, needles, leaves, seeds) and material of reference (tree stumps) in the forest. This was done with high probability (approximately 98– 99%).

19

Developing of DNA-marker to the Fusarium oxysporum f.sp. lycopersici resistance genes of tomato

63%

Amini J.

Journal of Plant Protection Research

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2009

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tom 49

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nr 2

175-178

EN

Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici is a destructive disease of tomato crops worldwide. The use of resistant varieties is the best strategy for disease control. In the present study we analyze eight tomato lines and hybrids for Fusarium wilt disease resistance by polymerase chain reaction. Total genomic DNA was extracted from young leaves of three-week-old plants of tomato. Results of PCR of eight tomato lines and hybrids indicated that there are one dominant heterozygote, two recessive homozygotes and five dominant homozygotes. Also, results of polymerase chain reaction showed that it needs less time and is cheaper. Also by using this method, it is possible to determine genotype of plant (homozygote or heterozygote) without presence of the pathogen. Therefore, PCR technique was used in the identification gene I2 conferring resistance to F. oxysporum f. sp. lycopersici.

PL

Więdnięcie pomidora wywoływane przez Fusarium oxysporum f. sp. lycopersici jest destruktywną chorobą w skali światowej. Najlepszą straegią zwalczania choroby jest wykorzystywanie odpornych odmian. W pracy przeanalizowano osiem linii i mieszańców pomidora na odporność przeciwko więdnięciu fuzaryjnemu, wykorzystując łańcuchową reakcję polimerazy (PCR).

20

DNA polymorphism in various goose lines by RAPD-PCR

63%

Bednarczyk M. , Siwek M. , Mazanowski A. , Czekalski P.

Folia Biologica

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2002

|

tom 50

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nr 1-2