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Molecular studies of seed aging effects in rye

100%

Niedzielski M. , Chwedorzewska K. , Puchalski J. , Bednarek P. T.

tom 01

Screening genetic disorder in cattle

86%

Citek J. , Rehout V. , Pavkova J. , Blahova B. , Hala K. , Strapak P.

tom 22

nr 2

DNA level in guard cells nuclei of Ornithogalum umbellatum ovary is 2C-4C

72%

Rogala K. , Kwiatkowska M. , Poplonska K.

nr 3

207-211

The DNA content after Feulgen reaction in the guard cells and epidermis of Omithogalum umbellatum ovary was cytophotometrically measured in different phases of flower development. Only in bud of flowers guard cells DNA content was 2C while in full blown flowers it was higher, between 2C-4C. This observation was supported by autoradiographic studies with 3H-thymidine which was incorporated into guard cell nuclei in the ovary epidermis of newly developed flowers. Thus DNA level in O. umbellatum guard cells was higher than those in other plants described in literature. On the other hand, DNA content in the epidermis cells increased gradually with ovary growth reaching the maximum level of 8C in some cells.

Spontaneous DNA damage in lymphocytes of fox species

72%

Grzesiakowska A. , Szeleszczuk O. , Kuchta-Gladysz M. , Otwinowska-Mindur A.

Folia Biologica

2019

tom 67

nr 1

Development of loop-mediated isothermal amplification [LAMP]-based SNP markers for shelf-life in melon [Cucumis melo L.]

72%

Fukuta S. , Mizukami Y. , Ishida A. , Kanbe M.

nr 4

In this study, LAMP markers linked to shelf-life in melon (Cucumis melo L.) were developed by converting a cleaved amplified polymorphic sequences (CAPS) marker (C2). The CAPS-PCR fragments from the long-shelf-life melon (0-3) and short-shelf-life melon (Nat-2) were cloned and sequenced to construct LAMP primers. A single nucleotide polymorphism (SNP) was identified between 0-3 and Nat-2. LAMP primers were designed to detect the SNP. In the LAMP reaction to detect long-shelf-life melon, the turbidity of the templates using 0-3, F1, homozygous long-shelf-life F2 lines and heterozygous long-shelf-life F2 lines started to increase after 40 min. In contrast, the turbidity of Nat-2 and homozygous short-shelf-life F2 lines did not increase even after 90 min. In the LAMP reaction to detect short-shelf-life melon, the turbidity of the templates using Nat-2, F1, homozygous short-shelf-life F2 lines and heterozygous long-shelf-life F2 lines started to increase after 40 min. But the turbidity of 0-3 and homozygous long-shelf-life F2 lines did not increase after 90 min. This attests to the high reliability and usefulness of LAMP for marker-assisted selection.

DNA changes induced by gamma-irradiation in onion

58%

Habdas H. , Staniaszek M.

1998

tom 1696