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nr 2
EN
The ornamental Tagetes minuta is a herbaceous plant of the Asteraceae family. T. minuta, a species native to southern South America, is used as a condiment, as a refreshing beverage, and for medicinal purposes. In 2011, disease symptoms of yellowing, root and foot rot, drying of leaves, and plant death were observed in an ornamental marigold (T. minuta) greenhouse in Fars province. The infected plants were collected and transferred to a laboratory. Samples were washed, cut into small pieces, surface disinfested with a 0.5% NaClO solution, and cultured on Potato Dextrose Agar (PDA) acidified to pH 4.5 with 0.5% lactic acid. Based on morphological characters, the causal agent was identified as Cylindrocarpon destructans. To confirm morphological identification, DNA was extracted from isolates using a genomic DNA purification Kit. The region of internal transcribed spacers 1, 2, and 5.8S genes of rDNA were amplified using the ITS4 and ITS1 universal primer set. Fragments of 600 bp were recovered from PCR, purified, sequenced, edited, and deposited in GenBank. The isolates had a 100% identity with all the compared C. destructans sequences. The pathogenicity tests were done with a suspension of 1 × 106 conidia per ml homogenised in sterile water. The symptoms on inoculated plants were similar to those previously observed and the fungus was reisolated from the inoculated plants. This is the first documented report of C. de-structans as a cause of root and foot rot disease on T. minuta in Iran.
EN
In 1997- 1998 the effect of manure, straw and mustard on fungi parasitization of eggs of beet cyst nematode in three years sugar beet rotation was investigated. The highest eggs parasitization by fungi was observed inside cysts from plots with spring barley cultivation and sugar beet as a forecrop. Straw fertilizer favored more fungi parasitization than manure. Three species of nematophagous fungi were isolated from eggs: Cylindrocarpon destructans, Paecilomyces lilacinus and Verticillium chlamydosporium.
PL
W latach 1997-1998 na polach Rolniczego Zakładu Doświadczalnego w Koniczynce pod Toruniem badano stopień spasożytowania jaj mątwika burakowego przez grzyby w zależności od nawożenia pól słomą, gorczycą i obornikiem, podczas uprawy buraka cukrowego w 3-letniej rotacji. Największe spasożytowanie jaj przez grzyby obserwowano na poletkach, na których uprawiano jęczmień jary w rok po uprawie buraka cukrowego. Spasożytowanie jaj było ponadto większe na poletkach nawożonych słomą niż na poletkach nawożonych obornikiem. Na poletkach, na których uprawiano burak cukrowy spasożytowanie jaj nie było już istotnie uzależnione od rodzaju nawożenia, co jest związane z cyklem rozwojowym szkodnika. Izolowano trzy gatunki grzybów nicieniobójczych, które redukowały liczebność populacji szkodnika na polu. Były to: Cylindrocarpon destructans, Paecilomyces lilacinus i Verticillium chlamydosporium.
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2002
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tom 48
EN
The aims of the study were to identify the fungi associated with the decay of thin roots (1-5 mm) of 1, 3 and 15-year-old Fraxinus excelsior plants and to estimate the pathogenicity of certain fungi. In total, 400 fungal isolations were performed from which 334 cultures were obtained. Altogether, 26 species of fungi were identified. The following species were most frequently obtained, independently of plant age and origin: Alternaria alternata, Chaetomium globosum, Cryptosporiopsis sp., Fusarium oxysporum, F. solani and Cylindrocarpon destructans. The last three of these were then used in an inoculation test. F. oxysporum caused the death of over 80% of 2-month-old seedlings grown in boxes containing perlite and kept in a growth chamber.
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