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EN
RP-HPLC (reversed-phase high-performance liquid chromatography) was used to analyse chlorophyll and carotenoid pigments in cyanobacteria and algae from the Baltic Sea, belonging to different taxonomic groups. The following species were used: Cyclotella meneghiniana - diatom, Oocystis submarina - green alga and Phormidium amphibium - cyanobacterium. Investigations on a favourable method of chlorophyll and carotenoid pigment separation have been carried out. This method allowed to separate the following pigments: lutein and zeaxanthin; diadinoxanthin and fucoxanthin; chlorophyll c1 and c2, into sharp peaks. It could be presumed that the method would be useful and universal in the identification of pigments in other algal and cyanobacteria species and natural phytoplankton assemblages.
2
Content available remote Effect of iron limitation on cells of the diatom Cyclotella meneghiniana Kützing
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EN
The response of the Baltic diatom Cyclotella meneghiniana to iron deficiency was examined. The following growth parameters were measured: cell number, chlorophyll a and protein content. The results demonstrate the ability of this diatom to grow well with minimal iron availability; however, the rate of growth fell markedly at the lowest iron(III) concentration. The results of spectrophotometric chlorophyll a measurements and protein assays using the Lowry and Bradford methods indicated a significant decrease in their quantities. Iron may therefore be an important regulatory factor controlling the growth of diatom C. meneghiniana in an aquatic ecosystem.
EN
The response of the Baltic diatom Cyclotella meneghiniana to iron deficiency was examined. The following growth parameters were measured: cell number, chlorophyll a and protein content. The results demonstrate the ability of this diatom to grow well with minimal iron availability; however, the rate of growth fell markedly at the lowest iron(III) concentration. The results of spectrophotometric chlorophyll a measurements and protein assays using the Lowry and Bradford methods indicated a significant decrease in their quantities. Iron may therefore be an important regulatory factor controlling the growth of diatom C. meneghiniana in an aquatic ecosystem.
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