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Inductors and age modifications of rat liver cytochrome P-450

100%

Plewka A. , Plewka D. , Kaminski M.

nr 4

453-468

In this paper we showed age effect on hepatic level of cytochrome P-450.We discussed effects of inductors on this hemoproteine also.Cytochrome P-450 content increased to 1-month of life, but decreased to 4-month.In the oldest rats cytochrome P-450 increased again.Content of this hemoprotein was induced by phenobarbital and ?- naphthoflavone in all examined groups, especially strong in young animals.Induction effect of dexamethasone was observed in 4-20 month of life.d

Correlationof mATPase, cytochrome c oxidase and parvalbumin in rat frozen skeletal muscle fibres

80%

Zawadowska B. , Muntener M.

tom 45

97-101

Muscle fibre typing is conventionally performed on unfixed frozen sections. Within the particular classes of fibre types mATPase correlates well with the type of myosin heavy chain (MHC). However, investigation of other properties, e.g., parvalbumin (PV) level, cannot be determined on unfixed frozen sections. Determination of PV, which correlates with the relaxation time, is performed immunohistochemically on fixed tissue. By that method a comparison of mATPase activity and the amount of PV within the same muscle fibre is difficult. The present method, combining histochemistry of mATPase and cytochrome c oxidase with parvalbumin immunohistochemistry on consecutive sections, allows the distinction of six fibre types: I, IB, IIA, IIB, IIAB, IIX, and a C fibre population without division into IC and IIC fibres. The advantages and disadvantages of the present method are discussed.