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Identification of Brucella melitensis from camel’s blood by vitek2 and real time polymerase chain reaction

100%

Manivannan K. , Ramasamy M. , Sundaresan U. , Moustafa S. M. , Sherloumay , Mariyam S.

nr 1

94-101

Introduction and aim. Brucellosis is a zoonotic disease. Experimental clinical and laboratory diagnosis is still facing problems in identifying the organism. The present study will diagnose a Brucella infection in camel blood in Qatar using serological assays. Isolation and identification were performed on a camel blood sample. Brucella in bacterial isolates was determined by real-time polymerase chain reaction (RT-PCR) as a gold standard test. Material and methods. A total of 220 samples, 200 random serum samples, and 20 EDTA blood samples were selected among the above-mentioned random samples, and 20 serum samples from camel handlers were collected from Al Shahaniya province, Qatar. The Rose Bengal test (RBT), buffered antigen plate agglutination test (BAPAT), and enzyme linked immunosorbent assay (cELISA) for the monoclonal antibody in serum samples were performed using commercially available kits. For the molecular detection of Brucella, conventional PCR and real-time PCR (GPS kit) were used for the genus-specific insertion sequence IS711. Brucella melitensis (MICROBOSS Hightech GmbH kit) was used to identify subspecies. Results. The results identified by vitek2 compact (30%) showed B. melitensis in 6 samples out of 20 isolates. Both conventional (66.67%) and RT-PCR (83.33%) analyses supported this, demonstrating the presence of Brucella. These tests also showed that Brucella species were present in Rose Bengal 182/200 (91%), BAPAT 182/200 (91%), and cELISA (90%) 180/200 in camel serum. Conclusion. To conclude, the prevalence of brucellosis in dromedary camels is higher in this region, and as a matter of urgency, measures should be taken to control the disease.

Comparison of rose bengal plate test antigens prepared from Brucella abortus, Brucella melitensis and Brucella suis

84%

Erganis O. , Hadimli H. H. , Solmaz H. , Corlu M.

nr 2

Aktualne dane na temat diagnostyki zakazen owiec i koz paleczkami Brucella

67%

Iwaniak W. , Szulowski K. , Pilaszek J. , Truszczynski M.

Medycyna Weterynaryjna

1997

tom 53

nr 07

377-379

Comparison of different PCR methods for detection of Brucella spp. in human blood samples

67%

Al-Ajlan H. , Ibrahim A. , Al-Salamah A.

nr 1

For detection of Brucella species by PCR four DNA extraction methods and four targets were compared using pure culture of Brucella melitensis and the best conditions were applied in clinical samples. It was found that the MagNA Pure LC method was the most efficient and sensitivemethod showing a positive PCR reaction with DNA extracted from as low as 25 and 100 CFU suspended in one ml blood and one ml water, respectively. Detection of Brucella spp. by conventional PCR was investigated using four different targets. The results indicated that The B4-B5 amplification method was the most sensitive one as it could amplify DNA extracted from as a low as 25 and 100 CFU/ml suspended in one ml water and blood, respectively. Furthermore real-time PCR was able to detect Brucella using DNA extracted from as low as 50 CFU/ml blood and 15 CFU/ml water, respectively. The best and optimum detection conditions were applied to the clinical samples. Evaluation of conventional PCR assays on blood specimens confirmed 72% of the results obtained by conventional blood culture methods with a specificity of 95%, while serum samples had a sensitivity of 54% and specificity of 100%. Real-time PCR was generally found to be more sensitive and specific for detecting Brucella spp. in blood and serum samples compared to conventional PCR. The real-time PCR done on blood specimens confirmed 77.5% of the results obtained by conventional blood culture methods with specificity of 100%, while 60% of serum samples were found to be positive with specificity of 100%. These results suggest that serum and blood analysis by conventional and real time PCR is a convenient and safe method for rapid and accurate diagnosis of brucellosis.

Epidemie pokarmowe u ludzi w krajach Unii Europejskiej w 2008 r.

59%

Wieczorek K. , Osek J.

nr 11

Choroby odzwierzęce i ich czynniki etiologiczne w raporcie Europejskiego Urzędu do spraw Bezpieczeństwa Żywności za 2007 r.

42%

Osek J. , Wieczorek K.

nr 05

Zoonozy i ich czynniki etiologiczne w swietle raportu EFSA za 2005 r.

42%

Osek J.

Życie Weterynaryjne

2007

tom 82

nr 04

294-301