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A novel, rapid, and simple PMA-qPCR method for detection and counting of viable Brucella organisms

100%

Zhang S.-J. , Wang L.-L. , Lu S.-Y. , Hu P. , Li Y.-S. , Zhang Y. , Chang H.-Z. , Zhai F.-F. , Liu Z.-S. , Li Z.-H. , Ren H.-L.

tom 64

nr 2

Comparative investigations of urease test and Brucella agar plating in the diagnosis of Helicobacter pylori infections

100%

Grodowska A. , Dzieniszewski J. , Jarosz M. , Popowski J. , Kafel S.

tom 48

nr 1

Comparison of different PCR methods for detection of Brucella spp. in human blood samples

80%

Al-Ajlan H. , Ibrahim A. , Al-Salamah A.

nr 1

For detection of Brucella species by PCR four DNA extraction methods and four targets were compared using pure culture of Brucella melitensis and the best conditions were applied in clinical samples. It was found that the MagNA Pure LC method was the most efficient and sensitivemethod showing a positive PCR reaction with DNA extracted from as low as 25 and 100 CFU suspended in one ml blood and one ml water, respectively. Detection of Brucella spp. by conventional PCR was investigated using four different targets. The results indicated that The B4-B5 amplification method was the most sensitive one as it could amplify DNA extracted from as a low as 25 and 100 CFU/ml suspended in one ml water and blood, respectively. Furthermore real-time PCR was able to detect Brucella using DNA extracted from as low as 50 CFU/ml blood and 15 CFU/ml water, respectively. The best and optimum detection conditions were applied to the clinical samples. Evaluation of conventional PCR assays on blood specimens confirmed 72% of the results obtained by conventional blood culture methods with a specificity of 95%, while serum samples had a sensitivity of 54% and specificity of 100%. Real-time PCR was generally found to be more sensitive and specific for detecting Brucella spp. in blood and serum samples compared to conventional PCR. The real-time PCR done on blood specimens confirmed 77.5% of the results obtained by conventional blood culture methods with specificity of 100%, while 60% of serum samples were found to be positive with specificity of 100%. These results suggest that serum and blood analysis by conventional and real time PCR is a convenient and safe method for rapid and accurate diagnosis of brucellosis.

Bruceloza - choroba odzwierzeca

80%

Pilaszek J. , Pilaszek M.

nr 11

44-47

Diagnosis of bovine brucellosis using traditional serological techniques and fluorescence polarisation assay

80%

Weiner M. , Iwaniak W. , Zlotnicka J. , Szulowski K.

nr 4

The aim of this study was the application of fluorescence polarisation assay (FPA) for the examination of bovine sera and comparison of the results of the assay with the results of Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), and ELISA. Six hundred thirty-five sera from cattle, including 300 sera from healthy animals, 32 sera from animals regarded as serologically positive for brucellosis and culled, and 303 sera originated from confirmatory investigations were used. All sera originating from healthy animals, negative in ELISA, RBT, SAT, and CFT were also negative in FPA. Among 303 sera from confirmatory investigations, 269 were positive in both RBT and SAT, 21 were positive in SAT and remaining 13 were RBT-positive only. Only two sera, one positive in both tests (RBT, SAT) and one SAT-positive, were also positive in FPA. Among 32 sera originated from animals regarded as serologically positive, which reacted in RBT, SAT, and CFT, 14 gave positive results in ELISA, whereas 18 were negative. Among these ELISA-positive sera, 13 were also positive in FPA. All samples positive in SAT, RBT, and CFT, and negative in ELISA, were also negative in FPA.

Praktyczne zastosowanie pomiaru pH mleka surowego do oceny jego swiezosci

70%

Grega T.

Przegląd Mleczarski

1995

nr 06

167-170