Wyniki wyszukiwania - Biblioteka Nauki

1

Expression profiles of toll-like receptors in the differentiation of an infection with Borrelia burgdorferi sensu lato spirochetes

100%

Dudek S. , Ziolko E. , Kimsa-Dudek M. , Solarz K. , Mazurek U. , Wierzgon A. , Kokot T. , Muc-Wierzgon M.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

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nr 2

2

Why is there still no human vaccine against Lyme borreliosis?

100%

Skotarczak B.

Folia Biologica

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2015

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tom 63

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nr 3

3

Infections caused by Borrelia burgdorferi sensu lato

100%

Tokarska-Rodak M.

Health Problems of Civilization

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2016

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tom 10

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nr 2

EN

It is justified to systematically monitor the number of those infected with Lyme disease in European countries and conduct the research on spreading of genospecies B. burgdorferi s.l. in animal reservoirs and vectors. Moreover, it is essential to take action for increasing the society’s awareness of tick-borne diseases prophylaxis. It is very important due to the observed increase in the number and activeness of ticks which leads to the increased risk of infection with spirochetes Borrelia burgdorferi sensu lato and other pathogens transmitted by these vectors.

4

The risk of exposure to Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Babesia sp. and co-infections in Ixodes ricinus ticks on the territory of Niepolomice Forest (southern Poland)

84%

Asman M. , Nowak M. , Cuber P. , Strzelczyk J. , Szilman E. , Szilman P. , Trapp G. , Siuda K. , Solarz K. , Wiczkowski A.

Annals of Parasitology

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2013

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tom 59

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nr 1

EN

Niepołomice Forest is located about 20 kilometers east of Cracow (Małopolska province, southern Poland). Its natural and touristic values, as well as wide range of hosts occurring within indicate this to be an area of high risk of exposure to Ixodes ricinus and tick-borne diseases it transfers. I. ricinus is a common species in Poland and Europe. Its seasonal activity begins in Poland in the early spring, and ends with late autumn. A total number of 129 specimens of I. ricinus was collected by flagging in Niepołomice Forest. DNA was isolated by ammonia method from 30 randomly-selected individuals. PCR was used to detect tick-borne pathogens with primers specific for Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato and Babesia sp. Molecular studies confirmed the presence of all three pathogens in I. ricinus. A. phagocytophilum was found in 76.7%, Babesia sp., 60%, B. burgdorferi s. l., in 3.3% of studied ticks. A. phagocytophilum co-infection with Babesia sp., was found in 46.7% of the specimens. A co-infection of all three tested pathogens was recorded in one case (3.3%). In Poland the problem of tick-borne diseases is a growing issue, therefore people residing in southern Polish touristic areas should be informed about the prevention and protection against ticks.

5

Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from Southern Poland

84%

Strzelczyk J. K. , Gazdzicka J. , Cuber P. , Asman M. , Trapp G. , Golabek K. , Zalewska-Ziob M. , Nowak-Chmura M. , Siuda K. , Wiczkowski A. , Solarz K.

Acta Parasitologica

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2015

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tom 60

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nr 4

6

Nonspecific bacterial flora isolated from the body surface and inside Ixodes ricinus ticks

84%

Okla H. , Sosnowska M. , Jasik K. P. , Slodki J. , Wojtyczka R. D.

Polish Journal of Microbiology

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2012

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tom 61

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nr 3

EN

Ixodes ricinus and other representatives of the order Ixodida are vectors of typical pathogens: Borrelia burgdorferi sensu lato, Anaplasma phagocytophilium, Babesia spp., a tick-borne encephalitis virus, and other microorganisms which are important from a medical and veterinary point of view. The presented study focuses on the verification of nonspecific bacterial flora of I. ricinus. We analyzed ticks collected in a forest region in Silesia, an industrial district in Poland. Methods of classical microbiology and biochemical assays (API 20 NE test, API Staph test and MICRONAUT System) were used for isolation and identification of microorganisms living on the body surface of I. ricinus and inside ticks. The results show the presence of various bacteria on the surface and inside ticks’ bodies. During the study, we isolated Acinetobacter lwoffi, Pseudomonas fluorescens, Aeromonas hydrophila, Achromobacter denitrificans, Alcaligenes faecalis, Stenotrophomonas maltophilia, Pseudomonas oryzihabitans, Micrococcus spp., Kocuria varians, Staphylococcus lentus, Kocuria kristinae, Streptococcus pneumo- niae, Rhizobium radiobacter, Staphylococcus xylosus. Majority of the isolated species are non-pathogenic environmental microorganisms, but some of the isolated bacterial strains could cause severe infections.

7

Przegląd metod służących do wykrycia zakażenia Borrelia burgdorferi

84%

Czubasiewicz Z. , Katarzyna Strzelczyk J. , Krzakiewicz K.

Pediatria i Medycyna Rodzinna

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2013

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tom 9

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nr 2

144-149

EN

Lyme borreliosis is an infectious disease caused by Borrelia burgdorferi sensu lato spirochetes and is transmitted to humans by the bite of an infected tick from the Ixodidae family, e.g. Ixodes ricinus, Ixodes scapularis, Ixodes persulcatus. The same species are responsible for causing Lyme borreliosis: B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. bissettii and B. spielmanii. The infection develops in stages and has various manifestations in the human body. Lyme disease mainly affects the skin, joints, the nervous system and the heart. Different species are bound with different clinical manifestations: Borrelia afzelii usually cause acrodermatitis chronica athrophicans, Borrelia burgdorferi sensu stricto is a common agent of arthritis, neuroborreliosis can be caused by B. Burgdorferi sensu stricto, B. afzelii and B. garinii. Lyme disease is diagnosed based on symptoms, epidemiological history and laboratory tests, e.g. serological tests of the serum or PCR tests from patient tissues or ticks. The most common diagnostic method is the one recommended by the Robert Koch Institute in Berlin. It is a two-stage procedure based on testing by screening assay in the first stage (EIA, IIFA, ELISA tests) and verifying positive and borderline results by Western blot tests during the second stage. Due to high antigenic variability, Borrelia burgdorferi sensu lato spirochetes have an ability to avoid defence mechanisms of an infected organism. This is the main cause of problems with interpretation of the clinical symptoms, diagnostics results and also with treatment of this infection.

PL

Borelioza jest chorobą zakaźną wywoływaną przez krętki Borrelia burgdorferi sensu lato. Do zakażenia tą bakterią dochodzi zwykle podczas ukąszenia przez zarażonego kleszcza z rodziny Ixodidae, m.in. Ixodes ricinus, Ixodes scapularis, Ixodes persulcatus. Za czynniki etiologiczne boreliozy uznawane są następujące genogatunki: B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. bissettii oraz B. spielmanii. Bakteria ta w organizmie człowieka może atakować różne narządy: skórę, stawy, ośrodkowy układ nerwowy, serce. Poszczególne szczepy wiązane są z rozwojem różnych obrazów choroby: Borrelia afzelii powoduje zazwyczaj rozwój przewlekłego zanikowego zapalenia skóry, Borrelia burgdorferi sensu stricto jest najczęściej odpowiedzialna za rozwój zapalenia stawów, neuroboreliozę może powodować zarówno B. burgdorferi sensu stricto, B. afzelii, jak i B. garinii. Boreliozę diagnozuje się na podstawie objawów klinicznych, wywiadu epidemiologicznego oraz testów laboratoryjnych, takich jak testy serologiczne w surowicy, a także badania metodą PCR tkanek pacjenta lub organizmu kleszcza odpowiedzialnego za ukąszenie. Najczęściej praktykowanym postępowaniem diagnostycznym jest rekomendowana przez Instytut Roberta Kocha w Berlinie tzw. diagnostyka dwuetapowa boreliozy, polegająca na wykonaniu czułego testu przesiewowego w pierwszym etapie (testy EIA, IIFA, ELISA) oraz weryfikacji dodatnich i wątpliwych wyników w drugim etapie (test Western blot). Ze względu na możliwości zmiany swojej struktury antygenowej krętki Borrelia burgdorferi sensu lato mają zdolność do unikania mechanizmów obronnych zainfekowanego organizmu. Jest to główna przyczyna problemów w interpretacji objawów klinicznych, wyników badań laboratoryjnych, a także leczenia zakażenia tą bakterią.

8

Simultaneous occurrence of Borrelia miyamotoi, Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Rickettsia helvetica in Ixodes ricinus ticks in urban foci in Bratislava, Slovakia

84%

Vaculova T. , Derdakova M. , Spitalska E. , Vaclav R. , Chvostac M. , Rusnakova Taragelova V.

Acta Parasitologica

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2019

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tom 64

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nr 1

9

A serological study of antibodies to Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in the sera of healthy individuals collected two decades apart

84%

Kriz B. , Maly M. , Balatova P. , Kodym P. , Kurzova Z. , Daniel M. , Kybicova K.

Acta Parasitologica

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2018

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tom 63

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nr 1

10

The fruitful coexistence of Borrelia burgdorferi and Ixodes ticks

84%

Lewandowski D. , Urbanowicz A. , Figlerowicz M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 1

11

Coexistence of Borrelia burgdorferi s.l. genospecies within Ixodes ricinus ticks from Central and Eastern Poland

67%

Sytykiewicz H. , Karbowiak G. , Chorostowska-Wynimko J. , Szpechcinski A. , Supergan-Marwicz M. , Horbowicz M. , Szwed M. , Czerniewicz P. , Sprawka I.

Acta Parasitologica

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2015

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tom 60

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nr 4

12

Identification and characterization of 31 isolates of Borrelia burgdorferi [Spirochaetales, Spirochaetaceae] obtained from various hosts and vectors using PCR-RFLP and SDS-PAGE analysis

67%

Zakovska A. , Janouskovcova E. , Pejchalova K. , Halouzka J. , Dendis M.

Acta Parasitologica

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2008

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tom 53

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nr 2

EN

Borrelia burgdorferi sensu lato, the etiologic agent of Lyme borreliosis, circulates between ticks and vertebrate hosts. Two main genospecies typically occur in the Czech Republic Borrelia garinii and Borrelia afzelii, transmitted generally by Ixodes ricinus (L., 1758) ticks. The aim of our study was to identify spirochaete isolates focusing on Borrelia burgdorferi acquired from different sources: vectors (ticks), potential vectors (mosquitoes, small mites) and hosts (wild rodents). In the years 1996–2001 a total of 2398 ticks, 72 mites (from wild rodents), 2700 mosquito adults, 1798 mosquito larvae and organ parts (kidney and spleen) of 216 wild rodents were collected from seven localities in the Czech Republic. A total of 31 spirochaete strains were isolated: 13 strains from ticks, 1 strain from mite (Haemogamasus sp.), 15 strains from rodents, 1 strain from mosquito adults and 1 strain from mosquito larva. For the genospecies identification of these isolates PCR, PCR-RFLP was used and their characterization was also performed by SDS-PAGE. By nested PCR method all except one isolated strains were detected as Borrelia burgdorferi s.l. Following PCR-RFLP molecular analysis results, tick isolates were identified as B. garinii and B. afzelii, the strain isolated from the mite was identified as B. afzelii. This is the first isolated strain of B.b.s.l. from a different mite of infraorder Parasitiformes than tick. All of rodent isolates were identified as B. afzelii; mosquito adult isolate was identified as B. afzelii. Larval isolate from mosquito is spirochaete, but does not belong to Borrelia burgdorferi sensu lato group.